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159 results for " Immune checkpoint" - showing 150-159


Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CD28 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)

CD28, Polyclonal Antibody (Cat# AAA9142769)

Full Name
CD28 Rabbit pAb
Gene Names
CD28; Tp44
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using OX40L antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

OX40L, Polyclonal Antibody (Cat# AAA9143300)

Full Name
OX40L Rabbit pAb
Gene Names
TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of Mouse lung, using CD27 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

CD27, Polyclonal Antibody (Cat# AAA9142082)

Full Name
CD27 Rabbit pAb
Gene Names
CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
Reactivity
Mouse
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of U-87MG cells, using B7-H3/CD276 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)

B7-H3/CD276, Polyclonal Antibody (Cat# AAA9142666)

Full Name
B7-H3/CD276 Rabbit pAb
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunofluorescence (IF) (Immunofluorescence staining of fixed RAW264.7 cells with anti-Tim-3 antibody 2C12 (MBS488620) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 2C12 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)

Tim-3, Monoclonal Antibody (Cat# AAA488620)

Full Name
Anti-Tim-3 [2C12]
Gene Names
Havcr2; Tim3; TIM-3; Timd3
Reactivity
Mouse
Applications
FC/FACS, IF, IHC, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of RAW 264.7 cells using anti-Tim-4 antibody (MBS488622) 3A1. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of 3A1 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)

Tim-4, Monoclonal Antibody (Cat# AAA488622)

Full Name
Anti-Tim-4 [3A1]
Gene Names
Timd4; Tim4; TIM-4; B430010N18Rik
Reactivity
Mouse
Applications
FC/FACS, WB, EIA, IHC, IF, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467) (MBS488604) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of PO.3 (MBS488604) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488604, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

CD86, Monoclonal Antibody (Cat# AAA488605)

Full Name
Anti-CD86 [PO.3]
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Western Blot using anti-HVEM antibody HMHV-1B18 (MBS488601) Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 () at 0.01ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)

HVEM, Monoclonal Antibody (Cat# AAA488601)

Full Name
Anti-HVEM [HMHV-1B18]
Reactivity
Mouse
Applications
FC/FACS, WB, IP, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed RAW264.7 cells with anti-CD80 antibody RM80 (B467) (MBS488602) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RM80 (MBS488602) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488602, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

CD80, Monoclonal Antibody (Cat# AAA488603)

Full Name
Anti-CD80 [RM80]
Gene Names
Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
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