Western Blot (WB)
(Western blot analysis of extracts of U-87MG cells, using B7-H3/CD276 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)
Rabbit anti-Human, Mouse B7-H3/CD276 Polyclonal Antibody | anti-B7-H3/CD276 antibody
B7-H3/CD276 Rabbit pAb
IHC: 1:50-1:200
Western Blot (WB)
(Western blot analysis of extracts of U-87MG cells, using B7-H3/CD276 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of U-87MG cells, using B7-H3/CD276 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta using B7-H3/CD276 antibody at dilution of 1:150 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta using B7-H3/CD276 antibody at dilution of 1:150 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsil using B7-H3/CD276 antibody at dilution of 1:150 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsil using B7-H3/CD276 antibody at dilution of 1:150 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using B7-H3/CD276 antibody at dilution of 1:150 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using B7-H3/CD276 antibody at dilution of 1:150 (40x lens).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
CD276: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Belongs to the immunoglobulin superfamily. BTN/MOG family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 15q23-q24
Cellular Component: integral to membrane; external side of plasma membrane
Molecular Function: protein binding; receptor binding
Biological Process: cell proliferation; positive regulation of osteoblast differentiation; regulation of immune response; negative regulation of T cell proliferation; T cell activation; negative regulation of inflammatory response; negative regulation of interleukin-2 biosynthetic process; positive regulation of T cell proliferation; immune response; positive regulation of bone mineralization; positive regulation of interferon-gamma biosynthetic process; negative regulation of interferon-gamma biosynthetic process
Research Articles on B7-H3/CD276
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Product Notes
The B7-H3/CD276 cd276 (Catalog #AAA9142666) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B7-H3/CD276 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H3/CD276 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the B7-H3/CD276 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LAQGNASLRL QRVRVADEGS FTCFVSIRDF GSAAVSLQVA APYSKPSMTL EPNKDLRPGD TVTITCSSYQ GYPEAEVFWQ DGQGVPLTGN VTTSQMANEQ G. It is sometimes possible for the material contained within the vial of "B7-H3/CD276, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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