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2560 results for " CD3" - showing 100-150


Flow Cytometry (FC/FACS) (Human peripheral blood lymphocytes are stained with Anrti-Human CD3 Monoclonal Antibody(Alexa Fluor 647 Conjuaged)(filled gray histogram). Unstained lymphocytes (empty black histogram) are used as control.)

CD3, Monoclonal Antibody (Cat# AAA2556821)

Full Name
Anti-Human CD3 Monoclonal Antibody (Alexa Fluor 647 Conjuaged)
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
FC/FACS
Pricing
$145/100 Tests | $180/200 Tests | $115/50 Tests | $100/20 Tests | $805/5x200 Tests
Testing Data (Western blot analysis of Jurkat human acute T-cell leukemia whole cell lysate probed with Mouse anti Human CD3 antibody (MBS215051) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)

CD3, Monoclonal Antibody (Cat# AAA219799)

Full Name
RAT ANTI HUMAN CD3
Gene Names
CD3E; T3E; TCRE; IMD18
Applications
FC/FACS, IF
Pricing
$210/0.025 mg | $800/5x0.025 mg
Western Blot (WB) (Figure 1: Western blot analysis using KSHV ORF26 mouse mAb against TPA induced BCBL-1 cell lysate.)

KSHV ORF26, Monoclonal Antibody (Cat# AAA9602612)

Full Name
KSHV ORF26 Antibody
Reactivity
Human
Applications
WB, EIA
Purity
Affinity-Chromatography
Pricing
$260/0.05 mL | $325/0.1 mL | $400/0.2 mL | $1,750/5x0.2 mL
Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC (MBS211022))

CD3, Monoclonal Antibody (Cat# AAA213023)

Full Name
MOUSE ANTI HUMAN CD3
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Applications
FC/FACS
Pricing
$475/0.2 mg | $1,995/5x0.2 mg
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92. Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

Hsp70, Monoclonal Antibody (Cat# AAA801664)

Full Name
Hsp70 Antibody: ATTO 390
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat, Bovine, C.elegans, Canine, Chicken, Drosophilia, Carp, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep
Applications
WB, IP, EIA, ICC, IHC, FC/FACS
Pricing
$555/0.2 mg | $2,260/5x0.2 mg
Testing Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 647 )

CD3, Monoclonal Antibody (Cat# AAA213054)

Full Name
RAT ANTI MOUSE CD3
Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Applications
FC/FACS
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Flow Cytometry (FC/FACS) ( Flow-cytometry using anti-CD3 antibody 12F6 Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of 12F6 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)

CD3 epsilon, Monoclonal Recombinant Antibody (Cat# AAA488258)

Full Name
Anti-CD3 epsilon [12F6]
Reactivity
Human
Applications
FC/FACS
Purity
Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92. Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

Hsp70, Monoclonal Antibody (Cat# AAA804686)

Full Name
Hsp70 Antibody: HRP
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat, Bovine, C.elegans, Canine, Chicken, Drosophilia, Carp, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep
Applications
WB, IP, EIA, ICC, IHC, FC/FACS
Pricing
$530/0.2 mg | $2,155/5x0.2 mg
Testing Data (Published customer image: Qualitative and quantitative flow cytometric analysis of lymphocyte populations in draining lymph nodes. A: Representative FACS plot of CD4+ CD8+ staining used to count T-helper cells, cytotoxic T-cells and CD4+ CD8+ double positive T-lymphocytes. Events acquired: 2x105. B: FACS plot example for B-cell detection. C: Representative FACS plot for NK cell assessment. NK-T cell were confirmed by CD3 expression (not shown). Bar diagrams: Cumulative results for the quantification of major and minor lymphocyte populations in draining LN of cornea transplanted animals. An asterisk (*) indicates statistical significance at p=0.05 determined by Mann -Whitney U-Test. Allo-Tx-d7 - animals allo-grafted and analyzed at day 07 post op, n=6; allo-Tx-rej - animals displaying allo rejection of grafted corneas analyzed after the onset of rejection, n=5; syn-Tx-d7 - syngeneically grafted animals analyzed at day 7 post-op, n=3; syn-Tx-LT - syn-grafted long-term survivors analyzed at the end of the observation period at day 42; n=3.Maenz M, Morcos M, Ritter T. A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model. Mol Vis. 2011 Feb 8;17:420-9.)

CD8 ALPHA, Monoclonal Antibody (Cat# AAA211033)

Full Name
MOUSE ANTI RAT CD8 ALPHA:FITC
Applications
FC/FACS
Pricing
$265/0.05 mg | $1,035/5x0.05 mg
Testing Data (Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin (MBS210477))

CD25, Monoclonal Antibody (Cat# AAA213821)

Full Name
MOUSE ANTI RAT CD25:RPE
Gene Names
Il2ra; IL2RAC
Applications
FC/FACS
Pricing
$510/100 Tests | $2,140/5x100 Tests
Immunohistochemistry (IHC) (MBS9600688 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

LCP1, Polyclonal Antibody (Cat# AAA9600688)

Full Name
Phospho-LCP1 (Tyr28) Antibody
Gene Names
LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Immunocytochemistry (ICC)/Immunofluorescence (IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 . Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Centre and Inst. Of Oncology, Poland.)

HSP70, Monoclonal Antibody (Cat# AAA8001855)

Full Name
HSP70 Antibody
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat, Bovine, Caenorhabditis elegans, Dog, Chicken, Fruit Fly, Carp, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep
Applications
WB, IHC, ICC, IF, EIA, FC/FACS, IEM, BL, AM
Purity
Protein G Purified
Pricing
$455/0.2 mg | $1,815/5x0.2 mg
Testing Data (Western blot analysis of Jurkat human acute T-cell leukemia whole cell lysate probed with Mouse anti Human CD3 antibody (MBS215051) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)

CD3, Monoclonal Antibody (Cat# AAA215052)

Full Name
RAT ANTI HUMAN CD3:FITC
Gene Names
CD3E; T3E; TCRE; IMD18
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92. Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

Hsp70, Monoclonal Antibody (Cat# AAA801280)

Full Name
Hsp70 Antibody
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat, Bovine, C.elegans, Canine, Chicken, Drosophilia, Carp, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep
Applications
WB, IP, EIA, ICC, IHC, FC/FACS
Pricing
$280/0.05 mg | $450/0.2 mg
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92. Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

HSP70, Monoclonal Antibody (Cat# AAA807082)

Full Name
Mouse Anti-Human HSP70 Monoclonal IgG
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat, Bovine, C. elegans, Dog, Chicken, Drosophila, Carp, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep
Applications
WB, IHC, ICC, IF, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$540/0.2 mg | $2,180/5x0.2 mg
Immunocytochemistry (ICC)/Immunofluorescence (IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 . Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Centre and Inst. Of Oncology, Poland.)

HSP70, Monoclonal Antibody (Cat# AAA8001854)

Full Name
HSP70 Antibody
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat, Bovine, Caenorhabditis elegans, Dog, Chicken, Fruit Fly, Carp, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep
Applications
WB, IHC, ICC, IF, EIA, FC/FACS, IEM, BL, AM
Purity
Protein G Purified
Pricing
$280/0.05 mg | $1,015/5x0.05 mg

CD3 epsilon, Monoclonal Recombinant Antibody (Cat# AAA488257)

Full Name
Anti-CD3 epsilon [12F6]
Reactivity
Human
Applications
FC/FACS
Purity
Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg

CD3 epsilon, Monoclonal Recombinant Antibody (Cat# AAA488282)

Full Name
Anti-CD3 epsilon [OKT-3 (muromonab)]
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg

CD3, Monoclonal Antibody (Cat# AAA2553772)

Full Name
Anti-Mouse CD3 Monoclonal Antibody (PercP Conjugated)
Gene Names
Cd3d; T3d
Reactivity
Mouse
Applications
FC/FACS
Pricing
$100/100 Tests | $120/200 Tests | $85/50 Tests | $160/500 Tests | $720/5x500 Tests
Flow Cytometry (FC/FACS) ( Flow-cytometry using anti-CD3epsilon antibody Mouse lymphocytes were stained with an anti-CD44 control or the rabbit-chimeric version of 500A2 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)

CD3 epsilon, Recombinant Antibody (Cat# AAA488076)

Full Name
Anti-CD3 epsilon [500A2]
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD127 Monoclonal Antibody(AF647 Conjuaged) and Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjuaged). Splenocytes stained with Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjuaged)(right) are used as control.)

CD127/IL-7RA, Monoclonal Antibody (Cat# AAA2558180)

Full Name
Anti-Mouse CD127/IL-7RA Monoclonal Antibody (AF647 Conjugated) [A7R34]
Gene Names
Il7r; CD127; IL-7Ralpha
Reactivity
Mouse
Applications
FC/FACS
Pricing
$165/0.05 mg | $215/0.1 mg | $290/0.2 mg | $130/0.025 mg | $1,305/5x0.2 mg
Western Blot (WB) (Figure 1: Western blot analysis using CD247 mAb against CD247-hIgGFc transfected HEK293 cell lysate.)

CD247, Antibody (Cat# AAA850383)

Full Name
CD247 Antibody
Gene Names
CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
Reactivity
Human
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Fluorophore (Conjugation: APCRecommended usage:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results or refer to the dilution we used in the experiment.)

CD3epsilon, Monoclonal Antibody (Cat# AAA2558698)

Full Name
Anti-Mouse CD3epsilon Monoclonal Antibody (APC Conjugated) [145-2C11]
Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Reactivity
Mouse
Applications
FC/FACS
Pricing
$105/0.05 mg | $130/0.1 mg | $175/0.2 mg | $85/0.025 mg | $785/5x0.2 mg
Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC (MBS211022))

CD3, Monoclonal Antibody (Cat# AAA216379)

Full Name
MOUSE ANTI HUMAN CD3:RPE
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Applications
FC/FACS
Pricing
$240/25 Tests | $930/5x25 Tests
Western Blot (WB) (Western blot analysis of Jurkat using CD3D antibody. The lane on the left is treated with the antigen-specific peptide.)

CD3D, Polyclonal Antibody (Cat# AAA9607028)

Full Name
CD3D Antibody
Gene Names
CD3D; T3D; IMD19; CD3-DELTA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from NIH/3T3 cells using KLRK1 antibody.)

KLRK1, Polyclonal Antibody (Cat# AAA9606270)

Full Name
KLRK1 Antibody
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of Acetylated-Lysine, using Acetyl Lysine antibody.)

Acetyl-Lysine, Polyclonal Antibody (Cat# AAA9608308)

Full Name
Acetyl-Lysine Antibody
Reactivity
Human
Applications
WB, EIA
Purity
Immunogen affinity purified
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of CD3G using HT29 whole cell lysates)

CD3G, Polyclonal Antibody (Cat# AAA9608555)

Full Name
CD3G Antibody
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 647 )

CD3, Monoclonal Antibody (Cat# AAA213055)

Full Name
RAT ANTI MOUSE CD3
Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Applications
FC/FACS
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
SDS-Page (SDS-PAGE Analysis Purified CD72 Mouse Monoclonal Antibody (BU40). Confirmation of Purity and Integrity of Antibody.)

CD72, Monoclonal Antibody (Cat# AAA4381445)

Full Name
CD72 (B Cell Marker)
Gene Names
CD72; LYB2; CD72b
Reactivity
Human
Applications
EIA, FC/FACS, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western blot analysis of extracts of Jurkat cells, using Phospho-LAT-Y171 antibody at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2mM) for 2 minutes after serum-starvation overnight or treated by CD-3 (10 ug/mL) for 2 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 5s.)

LAT-Y171, Polyclonal Antibody (Cat# AAA9135662)

Full Name
Phospho-LAT-Y171 pAb
Gene Names
LAT; LAT1; pp36; IMD52
Reactivity
Human
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Testing Data (Figure A. FITC conjugated Mouse anti Pig CD5 and Mouse IgG1 isotype control detected with Goat anti Mouse IgG1:RPE . Figure B. FITC conjugated Mouse anti Pig CD5 and Mouse anti Pig SWC5 detected with Goat anti Mouse IgG1:RPE . All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)

SWC5, Monoclonal Antibody (Cat# AAA225692)

Full Name
Mouse Anti Pig SWC5
Reactivity
Pig
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$355/0.1 mg | $1,435/5x0.1 mg

CD27, Monoclonal Recombinant Antibody (Cat# AAA488376)

Full Name
Anti-CD27 [RM27-3E5]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
FC/FACS, IP
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS) (Rat splenocytes are stained with Anti-Rat CD3 Monoclonal Antibody(APC Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

CD3, Antibody (Cat# AAA2559613)

Full Name
Anti-Rat CD3 Monoclonal Antibody (APC Conjugated)
Reactivity
Rat
Applications
FC/FACS
Pricing
$115/100 Tests | $130/200 Tests | $95/50 Tests | $185/500 Tests | $830/5x500 Tests
Immunofluorescence (IF) (MBS9605458 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

CD3EAP, Polyclonal Antibody (Cat# AAA9605458)

Full Name
CD3EAP Antibody
Gene Names
CD3EAP; ASE1; CAST; ASE-1; PAF49
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
ELISA (EIA) (ELISA of anti-CD27 antibody on CD27-Fc fusion protein, with a CD70 (CD27-L) competition assay. Binding curves of the rabbit IgG chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) antibody RM27-3E5 and isotype control (anti-Fluorescein antibody and then at 3-fold dilutions down to 0.17ng/mL (red line). A negative control assay using 4-1BB Fc-fusion protein was also performed (yellow line). Inhibition of the binding to Pr00160-1.9 (CD27) by (CD70) may be observed between 10,000 ng/mL and 123 ng/mL, whereas does not inhibit a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.)

CD27, Monoclonal Recombinant Antibody (Cat# AAA488375)

Full Name
Anti-CD27 [RM27-3E5]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
FC/FACS, IP
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC)

CD3, Monoclonal Antibody (Cat# AAA211022)

Full Name
MOUSE ANTI HUMAN CD3:FITC
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Flow Cytometry (FC/FACS) (Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD3 followed by Goat anti Mouse IgG (H/L):FITC )

CD3, Monoclonal Antibody (Cat# AAA224816)

Full Name
MOUSE ANTI PIG CD3: RPE
Gene Names
CD3E; CD3
Reactivity
Pig
Applications
FC/FACS
Pricing
$630/100 Tests | $2,680/5x100 Tests
Western Blot (WB) (Jurkat cells were subjected to SDS PAGE followed by western blot with MBS767689 (CD3D Antibody) at dilution of 1:1000)

CD3 delta, Monoclonal Antibody (Cat# AAA767689)

Full Name
CD3 delta Mouse Monoclonal
Gene Names
CD3D; T3D; IMD19; CD3-DELTA
Reactivity
Human, Mouse
Applications
EIA, WB
Purity
>=95% as determined by SDS-PAGE
Protein A+G purification
Pricing
$320/0.1 mg | $1,360/5x0.1 mg
Immunofluorescence (IF) (Figure 1: Immunofluorescence analysis of peripheral blood T cells using CD3 mouse mAb (A) and flow cytometric analysis of eripheral blood T cells using CD3 mouse mAb (B).)

CD3, Monoclonal Antibody (Cat# AAA9602696)

Full Name
CD3 Antibody
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
IF, ICC, EIA
Purity
Affinity-Chromatography
Pricing
$260/0.05 mL | $325/0.1 mL | $400/0.2 mL | $1,750/5x0.2 mL
Flow Cytometry (FC/FACS) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD3 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

CD3, Monoclonal Antibody (Cat# AAA2558090)

Full Name
Anti-Mouse CD3 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated)
Gene Names
Cd3d; T3d
Reactivity
Mouse
Applications
FC/FACS
Pricing
$155/0.05 mg | $190/0.1 mg | $275/0.2 mg | $125/0.025 mg | $1,230/5x0.2 mg
Testing Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 647 )

CD3, Monoclonal Antibody (Cat# AAA213843)

Full Name
RAT ANTI MOUSE CD3:RPE
Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Western Blot (WB)

CD3E, Monoclonal Antibody (Cat# AAA435183)

Full Name
CD3E Mouse mAb
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Testing Data (Published customer image: Qualitative and quantitative flow cytometric analysis of lymphocyte populations in draining lymph nodes. A: Representative FACS plot of CD4+ CD8+ staining used to count T-helper cells, cytotoxic T-cells and CD4+ CD8+ double positive T-lymphocytes. Events acquired: 2x105. B: FACS plot example for B-cell detection. C: Representative FACS plot for NK cell assessment. NK-T cell were confirmed by CD3 expression (not shown). Bar diagrams: Cumulative results for the quantification of major and minor lymphocyte populations in draining LN of cornea transplanted animals. An asterisk (*) indicates statistical significance at p=0.05 determined by Mann -Whitney U-Test. Allo-Tx-d7 - animals allo-grafted and analyzed at day 07 post op, n=6; allo-Tx-rej - animals displaying allo rejection of grafted corneas analyzed after the onset of rejection, n=5; syn-Tx-d7 - syngeneically grafted animals analyzed at day 7 post-op, n=3; syn-Tx-LT - syn-grafted long-term survivors analyzed at the end of the observation period at day 42; n=3.Maenz M, Morcos M, Ritter T. A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model. Mol Vis. 2011 Feb 8;17:420-9.)

CD8 ALPHA, Monoclonal Antibody (Cat# AAA211034)

Full Name
MOUSE ANTI RAT CD8 ALPHA:FITC
Applications
FC/FACS
Pricing
$645/0.5 mg | $2,755/5x0.5 mg

CD3 epsilon, Monoclonal Recombinant Antibody (Cat# AAA488226)

Full Name
Anti-CD3 epsilon [YTH 12.5]
Reactivity
Human
Purity
Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data (Figure A. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat IgG2a isotype control . Figure B. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat anti Canine CD5 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

IgG2a, Negative Control (Cat# AAA225675)

Full Name
Rat IgG2a Negative Control
Reactivity
Rat
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$215/0.1 mg | $810/5x0.1 mg

CD3 epsilon, Recombinant Antibody (Cat# AAA488075)

Full Name
Anti-CD3 epsilon [500A2]
Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD122 Monoclonal Antibody(PE/Cyanine5 Conjugated) and Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjugated). Splenocytes stained with Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjugated)(right) are used as control.)

CD122, Monoclonal Antibody (Cat# AAA2558222)

Full Name
Anti-Mouse CD122 Monoclonal Antibody (PE/Cyanine5 Conjugated) [5H4]
Gene Names
Il2rb; p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta
Reactivity
Mouse
Applications
FC/FACS
Pricing
$165/0.05 mg | $230/0.1 mg | $340/0.2 mg | $130/0.025 mg | $1,510/5x0.2 mg
Testing Data (Figure A. Pacific Blue conjugated Mouse anti Human CD3 and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. Pacific Blue conjugated Mouse anti Human CD3 and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD55 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

CD55, Monoclonal Antibody (Cat# AAA225631)

Full Name
Mouse Anti Human CD55: FITC
Gene Names
CD55; CR; TC; DAF; CROM; CHAPLE
Reactivity
Human
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 647 )

CD3, Monoclonal Antibody (Cat# AAA211042)

Full Name
RAT ANTI MOUSE CD3:FITC
Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
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