Mouse Protein Disulfide Isomerase A3 ELISA Kit | PDIA3 elisa kit

Mouse Protein Disulfide Isomerase A3 ELISA Kit

Cat. Num. AAA762542

• Gene Names: PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• Reactivity: Mouse
• Synonyms: Protein Disulfide Isomerase A3; Mouse Protein Disulfide Isomerase A3 ELISA Kit; PDIA3/ERp60/GRP58/Disulfide isomerase ER-60/endoplasmic reticulum P58/Endoplasmic reticulum resident protein 57/Endoplasmic reticulum resident protein 60/ER protein 60/ER60/ERP57/ERp5758 kDa glucose-regulated protein/ERp6058 kDa microsomal protein/ERp61/glucose regulated protein; 58kDa/GRP57/GRP58ER protein 57/HsT17083/P58/p58/phospholipase C-alpha/PI-PLC/protein disulfide isomerase family A; member 3/protein disulfide isomerase-associated 3/protein disulfide-isomerase A3; PDIA3 elisa kit

• Reactivity: Mouse
• Sequence Length: 505

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.391-25ng/ml
• Sensitivity: 0.234ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PDIA3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse PDIA3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse PDIA3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDIA3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse PDIA3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21361657
• NCBI GeneID: 2923
• NCBI Accession #: NP_005304.3
• NCBI GenBank Nucleotide #: NM_005313.4
• Molecular Weight: 56,782 Da
• NCBI Official Full Name: protein disulfide-isomerase A3
• NCBI Official Synonym Full Names: protein disulfide isomerase family A member 3
• NCBI Official Symbol: PDIA3
• NCBI Official Synonym Symbols: P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• NCBI Protein Information: protein disulfide-isomerase A3
• UniProt Protein Name: Protein disulfide-isomerase A3
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA3
• UniProt Synonym Gene Names: ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
• UniProt Entry Name: PDIA3_HUMAN

NCBI Description

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]

Uniprot Description

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.

Protein type: EC 5.3.4.1; Isomerase; Phospholipase; Chaperone; Endoplasmic reticulum; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; focal adhesion; melanosome; myelin sheath; nucleus

Molecular Function: cysteine-type endopeptidase activity; disulfide oxidoreductase activity; phospholipase C activity; protein binding; protein disulfide isomerase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cell redox homeostasis; cellular protein metabolic process; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; protein folding; protein import into nucleus; protein retention in ER; proteolysis; signal transduction