SDS-Page
(SDS-PAGE Analysis Purified CD72 Mouse Monoclonal Antibody (BU40). Confirmation of Purity and Integrity of Antibody.)
Mouse anti-Human CD72 Monoclonal Antibody | anti-CD72 antibody
CD72 (B Cell Marker)
Gene Names
CD72; LYB2; CD72b
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
CD72; Monoclonal Antibody; CD72 (B Cell Marker); B cell differentiation antigen CD72; CD72b; CD72c; Ly-19; Ly-32; Ly-m19; LYB2; Lymphocyte antigen 32; anti-CD72 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
BU40
Specificity
CD5 has been identified as a transmembrane glycoprotein that is expressed on 70% of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. Activation of T cells through the T cell receptor (TCR) results in tyrosine phosphorylation of CD5, and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation. CD5 associates with the TCR/CD3 chain, and with the Src family kinase, Lck p56. The C-type lectin superfamily member CD72 is a cell surface negative regulator of B cell activation from the pro-B through the mature B cell stage. CD72 serves as a receptor for CD5. The ability of lymphocytes to respond to antigenic or mitogenic stimulation utilizes both positive and negative regulatory proteins that influence the threshold for responsiveness. The human CD72 gene maps to chromosome 9p13.3 and encodes a transmembrane glycoprotein that contains an ITIM. Upon tyrosine phosphorylation, the CD72 ITIM recruits SH2-containing phosphatases such as SHP-1, resulting in downregulation of cell activation. CD72-/- mice contain hyperproliferative B cells.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
359
Applicable Applications for anti-CD72 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
ELISA: For coating, order without BSA
FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
K562 cel lysate. Tonsil or Lymph Node.
Immunogen
HF B1 plasmacytoid cell line.
Cellular Localization
Membrane
Hu-chromosome Location
9p13.3
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-Page
(SDS-PAGE Analysis Purified CD72 Mouse Monoclonal Antibody (BU40). Confirmation of Purity and Integrity of Antibody.)
SDS-Page
(SDS-PAGE Analysis Purified CD72 Mouse Monoclonal Antibody (BU40). Confirmation of Purity and Integrity of Antibody.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Lymph Node in Colon stained with CD72 Mouse Monoclonal Antibody (BU40).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Lymph Node in Colon stained with CD72 Mouse Monoclonal Antibody (BU40).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Lymph Node in Colon stained with CD72 Mouse Monoclonal Antibody (BU40).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Lymph Node in Colon stained with CD72 Mouse Monoclonal Antibody (BU40).)
References
Yoshida, Y., et al. 2015. Arthritis Rheumatol. 67: 1481-1490.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45kDa
NCBI Official Full Name
B-cell differentiation antigen CD72
NCBI Official Synonym Full Names
CD72 molecule
NCBI Official Symbol
CD72
NCBI Official Synonym Symbols
LYB2; CD72b
NCBI Protein Information
B-cell differentiation antigen CD72
UniProt Protein Name
B-cell differentiation antigen CD72
Protein Family
UniProt Gene Name
CD72
UniProt Entry Name
CD72_HUMAN
Uniprot Description
CD72: Plays a role in B-cell proliferation and differentiation.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p13.3
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding; receptor binding
Biological Process: axon guidance; signal transduction; cell adhesion
Research Articles on CD72
Similar Products
Product Notes
The CD72 cd72 (Catalog #AAA4381445) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD72 (B Cell Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD72 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. ELISA: For coating, order without BSA FC/FACS: 0.5-1ug/million cells IF: 0.5-1ug/ml Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the CD72 cd72 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD72, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
