WB (Western Blot)
(Detection of human and mouse AF9 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), and HEK293T (50ug), and mouse NIH 3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AF9 antibody AAA211042 (lot AAA211042-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse AF9 Polyclonal Antibody | anti-AF9 antibody
Rabbit anti-AF9 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
AF9, Antibody; Rabbit anti-AF9 Antibody, Affinity Purified; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3; YEATS domain-containing protein 3; protein AF-9; YEATS3; myeloid/lymphoid or mixed-lineage leukemia; translocated to 3; ALL1-fused gene from chromosome 9 protein; AF9; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; Protein AF-9; MLLT3, super elongation complex subunit; anti-AF9 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-AF9 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 200 and 250
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse AF9 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), and HEK293T (50ug), and mouse NIH 3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AF9 antibody AAA211042 (lot AAA211042-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse AF9 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), and HEK293T (50ug), and mouse NIH 3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AF9 antibody AAA211042 (lot AAA211042-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human AF9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa (50ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AF9 antibody AAA211042 (lot AAA211042-2) used for IP at 6 ug per reaction. AF9 was also immunoprecipitated rabbit anti-AF9 antibodies A300-597A and A300-597A. For blotting immunoprecipitated AF9, AAA211042 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human AF9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa (50ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AF9 antibody AAA211042 (lot AAA211042-2) used for IP at 6 ug per reaction. AF9 was also immunoprecipitated rabbit anti-AF9 antibodies A300-597A and A300-597A. For blotting immunoprecipitated AF9, AAA211042 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-AF9 antibody
The human AF9 gene is one of the most common fusion partner genes with the MLL (mixed lineage leukemia) gene resulting in AF9/MLL translocations and leukemogenesis. AF9 is highly homologous to the gene product of ENL, another common MLL fusion partner gene. The function of AF9 is not known but through studies of ENL it is proposed to be a nuclear transactivating factor.
Product Categories/Family for anti-AF9 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The AF9 mllt3 (Catalog #AAA211042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-AF9 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AF9 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the AF9 mllt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AF9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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