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3126 results for "cell-cycle-protein-kinase" - showing 1200-1250


Western Blot (WB) (Western blot analysis of Phospho-mTOR (S2448) expression in HEK293 cell lysate (MBS1750174).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )

mTOR, Monoclonal Antibody (Cat# AAA1750174)

Full Name
Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

CDK2 / Cyclin-Dependent Kinase 2, Recombinant Protein (Cat# AAA2903049)

Full Name
Human CDK2 / Cyclin-Dependent Kinase 2 Recombinant Protein
Gene Names
CDK2; CDKN2; p33(CDK2)
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE

Chk1, Polyclonal Antibody (Cat# AAA8521880)

Full Name
Chk1 Polyclonal Antibody
Gene Names
CHEK1; CHK1
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)

Cyclin Dependent Kinase 9 (CDK9), Antibody Pair Kit (Cat# AAA2099017)

Full Name
Human Cyclin Dependent Kinase 9 (CDK9) Antibody Pair Kit (with Standard)
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human
Applications
EIA, CLIA
Pricing
$1,470/10x96 Tests | $925/5x96 Tests | $1,000/5x96 Tests + MBS2090685 (Ab Pairs Support Pack 1, 5x96 Tests) | $1,595/10x96 Tests + MBS2090685 (Ab Pairs Support Pack 1, 10x96 Tests)
Western Blot (WB) (Figure 1. Western blot analysis of Chk1 using anti- Chk1 antibody (MBS1750555).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: SW620 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Chk1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Chk1 at approximately 54KD. The expected band size for Chk1 is at 54KD. )

Chk1, Polyclonal Antibody (Cat# AAA1750555)

Full Name
Anti-Chk1 Picoband Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis of Phospho-mTOR (S2481) expression in (1)293 cell lysate treated with LP; (2)293 cell lysate (MBS1750175).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )

mTOR, Monoclonal Antibody (Cat# AAA1750175)

Full Name
Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

Chk1, Polyclonal Antibody (Cat# AAA8521884)

Full Name
Chk1 Polyclonal Antibody
Gene Names
CHEK1; CHK1
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk1 (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.)

Chk1, Polyclonal Antibody (Cat# AAA9502987)

Full Name
Chk1 (Phospho-Ser345) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.)

p16INK4A, Monoclonal Antibody (Cat# AAA9435662)

Full Name
p16INK4A Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB) (Western blot analysis of PKM2 expression in HeLa cell lysate (MBS179577).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKM monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PKM )

PKM2, Monoclonal Antibody (Cat# AAA179577)

Full Name
Anti-PKM2 Rabbit Monoclonal Antibody
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate (MBS1750190).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AKT1 )

AKT1, Monoclonal Antibody (Cat# AAA1750190)

Full Name
Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from various samples, using Bub1 Antibody.Lane 1: Hela cells(heat shock treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(heat shock treatment),Lane 3: HepG2 cells(serum starvation treatment).)

Bub1, Polyclonal Antibody (Cat# AAA9613789)

Full Name
Bub1 Antibody
Gene Names
BUB1; BUB1A; BUB1L; hBUB1
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

Chk1, Polyclonal Antibody (Cat# AAA8519836)

Full Name
Chk1 (phospho Ser317) Polyclonal Antibody
Gene Names
CHEK1; CHK1
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Typical Testing Data/Standard Curve (for reference only)

Checkpoint Homolog (CHEK1), RTU ELISA Kit (Cat# AAA458351)

Full Name
Mouse Checkpoint Homolog (CHEK1) ELISA Kit
Gene Names
Chek1; Chk1; rad27; C85740
Reactivity
Mouse
Pricing
$655/96 Tests | $490/48 Tests | $5,785/10x96 Tests | $2,955/5x96 Tests

SLK, Polyclonal Antibody (Cat# AAA5304991)

Full Name
Rabbit polyclonal SLK antibody
Gene Names
SLK; LOSK; STK2; se20-9; bA16H23.1
Applications
IP, WB

CHK1 / CHEK1, Recombinant Protein (Cat# AAA2545110)

Full Name
Recombinant Human CHK1 / CHEK1 Protein (GST tag)
Gene Names
CHEK1; CHK1
Purity
> 95 % as determined by SDS-PAGE
Pricing
$535/0.02 mg | $660/0.05 mg | $2,970/5x0.05 mg
Western Blot (WB) (Western blot analysis of extracts from HeLa cells, using CDC7 antibody (MBS179583).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC7 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC7 )

CDC7, Monoclonal Antibody (Cat# AAA179583)

Full Name
Anti-CDC7 Rabbit Monoclonal Antibody
Gene Names
CDC7; Hsk1; CDC7L1; HsCDC7; huCDC7
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of CDK7 expression in MCF-7 cell lysate (MBS179366).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK7 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK7 )

CDK7, Monoclonal Antibody (Cat# AAA179366)

Full Name
Anti-CDK7 Rabbit Monoclonal Antibody
Gene Names
CDK7; CAK; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

Cyclin Dependent Kinase 9 (CDK9), Polyclonal Antibody (Cat# AAA2092071)

Full Name
Biotin-Linked Polyclonal Antibody to Cyclin Dependent Kinase 9 (CDK9)
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human
Applications
WB, IHC
Pricing
$165/0.1 mL | $205/0.2 mL | $340/0.5 mL | $420/1 mL | $1,210/5x1 mL

Cyclin Dependent Kinase 9 (CDK9), Polyclonal Antibody (Cat# AAA2043098)

Full Name
PE-Linked Polyclonal Antibody to Cyclin Dependent Kinase 9 (CDK9)
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human
Applications
WB, ICC, IHC, ELISA
Pricing
$240/0.1 mL | $310/0.2 mL | $590/0.5 mL | $720/1 mL | $2,030/5x1 mL

Cyclin Dependent Kinase 9 (CDK9), Polyclonal Antibody (Cat# AAA2043102)

Full Name
HRP-Linked Polyclonal Antibody to Cyclin Dependent Kinase 9 (CDK9)
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human
Applications
WB, ICC, IHC, ELISA
Pricing
$180/0.1 mL | $225/0.2 mL | $395/0.5 mL | $485/1 mL | $1,370/5x1 mL

CDK9, Polyclonal Antibody (Cat# AAA8529280)

Full Name
CDK9 Antibody
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot analysis of extracts from HT29 cells untreated or treated with UV, using Chk1 (Ab-345) antibody)

Chk1, Antibody (Cat# AAA8510100)

Full Name
Chk1 (Ab-345) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot analysis of Chk1 phosphorylation expression in MCF7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Chk1, Antibody (Cat# AAA8511191)

Full Name
Phospho-Chk1 (Ser317) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Immunogen affinity purified
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk1 antibody (left) or the same antibody preincubated with with blocking peptide (right))

Chk1, Polyclonal Antibody (Cat# AAA5309714)

Full Name
Chk1 antibody
Gene Names
CHEK1; CHK1
Applications
IHC, WB
Purity
Purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Western Blot (WB) (Western blot analysis of Cdc37 expression in (1) Jurkat cell lysate; (2) SW480 cell lysate (MBS179804).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC37 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC37 )

Cdc37, Monoclonal Antibody (Cat# AAA179804)

Full Name
Anti-Cdc37 Rabbit Monoclonal Antibody
Gene Names
CDC37; P50CDC37
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of p21 in (1) MCF-7 cell lysate; (2) PC-12 cell lysate (MBS179141).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDKN1A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDKN1A )

p21, Monoclonal Antibody (Cat# AAA179141)

Full Name
Anti-p21 Rabbit Monoclonal Antibody
Gene Names
CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of CDK1 in HeLa cell lysate (MBS179188).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK1 )

CDK1/Cdc2, Monoclonal Antibody (Cat# AAA179188)

Full Name
Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

CDK12, Polyclonal Antibody (Cat# AAA7108677)

Full Name
CDK12 Antibody, FITC conjugated
Gene Names
CDK12; CRK7; CRKR; CRKRS
Reactivity
Human
Applications
EIA
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot analysis of Phospho-CDK9 (Ser347) in lysates of NIH-3T3 Forskolin 40nM 30', using Phospho-CDK9 (Ser347) Antibody(13165).)

CDK9, Polyclonal Antibody (Cat# AAA9429317)

Full Name
CDK9 (Phospho-Ser347) Antibody
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB) (Western blot analysis of Mouse Liver showing detection of ~16.5, 11.2 kDa CDKN2A protein using Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16.5, 11.2 kDa.)

CDKN2A, Polyclonal Antibody (Cat# AAA8001392)

Full Name
CDKN2A Antibody: Biotin
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB) (Western blot analysis of Phospho-AKT1 (T450) expression in (1) 3T3 cell lysate treated with Lambda Phosphatase; (2) 3T3 cell lysate (MBS1750188).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AKT1 )

AKT1, Monoclonal Antibody (Cat# AAA1750188)

Full Name
Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of mTOR expression in Jurkat cell lysate (MBS179041).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )

mTOR/Tor, Monoclonal Antibody (Cat# AAA179041)

Full Name
Anti-mTOR/Tor Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Chk1 phosphorylation expression in UV treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Chk1, Antibody (Cat# AAA8511188)

Full Name
Phospho-Chk1 (Ser296) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB, IHC
Purity
Immunogen affinity purified
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot analysis of CDK8 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate (MBS179673).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK8 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK8 )

CDK8, Monoclonal Antibody (Cat# AAA179673)

Full Name
Anti-CDK8 Rabbit Monoclonal Antibody
Gene Names
CDK8; K35
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Chk2 expression in (1) HeLa cell lysate; (2) 293T cell lysate (MBS179235).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHEK2 )

Chk2, Monoclonal Antibody (Cat# AAA179235)

Full Name
Anti-Chk2 Rabbit Monoclonal Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

CDK9, Blocking Peptide (Cat# AAA428857)

Full Name
CDK9 Immunizing Peptide
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Mouse
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Pricing
$215/0.1 mg
Typical Testing Data/Standard Curve (for reference only)

Cyclin Dependent Kinase 1 (CDK1), RTU ELISA Kit (Cat# AAA458278)

Full Name
Human Cyclin Dependent Kinase 1 (CDK1) ELISA Kit
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human
Pricing
$575/96 Tests | $435/48 Tests | $5,050/10x96 Tests | $2,590/5x96 Tests
Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells, using Chk1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Chk1, Polyclonal Antibody (Cat# AAA9503824)

Full Name
Chk1 Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
IF, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB) (Western blot analysis of lysates from HT-29 cells, treated with UV, using Chk1 Antibody. The lane on the right is blocked with the synthesized peptide.)

Chk1, Polyclonal Antibody (Cat# AAA9504094)

Full Name
Chk1 Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL

Cyclin Dependent Kinase 1 (CDK1), Antibody Pair Kit (Cat# AAA2101014)

Full Name
Mouse Cyclin Dependent Kinase 1 (CDK1) Antibody Pair Kit (with Standard)
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Mouse
Applications
EIA, CLIA
Pricing
$1,505/10x96 Tests | $950/5x96 Tests | $1,020/5x96 Tests + MBS2090685 (Ab Pairs Support Pack 1, 5x96 Tests) | $1,630/10x96 Tests + MBS2090685 (Ab Pairs Support Pack 1, 10x96 Tests)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Chk1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Chk1, Polyclonal Antibody (Cat# AAA9504093)

Full Name
Chk1 Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The picture on the right is blocked with the phospho peptide.)

Chk1, Polyclonal Antibody (Cat# AAA9502714)

Full Name
Chk1 (Phospho-Ser301) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
WB, IF, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL

CDK12 / Cyclin-Dependent Kinase 12, Recombinant Protein (Cat# AAA2903489)

Full Name
Human CDK12 / Cyclin-Dependent Kinase 12 Recombinant Protein
Gene Names
CDK12; CRK7; CRKR; CRKRS
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Western Blot (WB) (Western blot analysis of Mouse Liver showing detection of ~16.5, 11.2 kDa CDKN2A protein using Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16.5, 11.2 kDa.)

CDKN2A, Polyclonal Antibody (Cat# AAA8001394)

Full Name
CDKN2A Antibody: HRP
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB) (Western blot analysis of Phospho-CDC37 (S13) expression in (1) Jurkat cell lysate treated with Alkaline Phosphatase; (2) Jurkat cell lysate (MBS1750273).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC37 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC37 )

CDC37, Monoclonal Antibody (Cat# AAA1750273)

Full Name
Anti-Phospho-CDC37 (S13) Rabbit Monoclonal Antibody
Gene Names
CDC37; P50CDC37
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of CDK1 expression in HeLa cell lysate (MBS179190).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK1 )

CDK1/Cdc2, Monoclonal Antibody (Cat# AAA179190)

Full Name
Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of CDK1 expression in (1)A431 cell lysate;(2)HeLa cell lysate (MBS179191).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK1 )

CDK1/Cdc2, Monoclonal Antibody (Cat# AAA179191)

Full Name
Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

HIVEP3, Polyclonal Antibody (Cat# AAA9630376)

Full Name
HIVEP3 Antibody
Gene Names
HIVEP3; KRC; KBP1; SHN3; ZAS3; KBP-1; ZNF40C; Schnurri-3
Reactivity
Human, Mouse
Applications
EIA, peptide
Purity
Peptide Affinity Purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from mouse brain, using MLK3 (Phospho-Thr277+Ser281) Antibody.)

MLK3, Polyclonal Antibody (Cat# AAA9614080)

Full Name
Phospho-MLK3 (Thr277+Ser281) Antibody
Gene Names
MAP3K11; MLK3; PTK1; SPRK; MLK-3; MEKK11
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Peptide affinity purification
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
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