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3126 results for "cell-cycle-protein-kinase" - showing 1250-1300
Chk1, Monoclonal Antibody (Cat# AAA8534090)
Full Name
Phospho-Chk1 (S296)
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB, IHC
Purity
ProA affinity purified
Pricing
$350/0.1 mL | $570/0.1 mL (Biotin) | $1,515/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Checkpoint Kinase 1 (CHEK1), Antibody Pair Kit (Cat# AAA2104248)
Full Name
Mouse Checkpoint Kinase 1 (CHEK1) Antibody Pair Kit (with Standard)
Reactivity
Mouse
Applications
EIA, CLIA
Pricing
$1,650/10x96 Tests | $1,040/5x96 Tests | $1,110/5x96 Tests + MBS2090685 (Ab Pairs Support Pack 1, 5x96 Tests) | $1,770/10x96 Tests + MBS2090685 (Ab Pairs Support Pack 1, 10x96 Tests)
Western Blot (WB)
(WB Suggested Anti-CDC2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Spleen)
CDC2, Polyclonal Antibody (Cat# AAA3213871)
Full Name
CDC2 antibody - middle region
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Mouse Liver showing detection of ~16.5, 11.2 kDa CDKN2A protein using Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16.5, 11.2 kDa.)
CDKN2A, Polyclonal Antibody (Cat# AAA8001390)
Full Name
CDKN2A Antibody: Alkaline Phosphatase
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human beast carcinoma, using CDK2 Antibody(MBS179156)CDK2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK2 Antibody (MBS179156)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
CDK2, Monoclonal Antibody (Cat# AAA179156)
Full Name
Anti-CDK2 Rabbit Monoclonal Antibody
Gene Names
CDK2; CDKN2; p33(CDK2)
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
CHEK1-S345, Polyclonal Antibody (Cat# AAA8537022)
Full Name
Phospho-CHEK1-S345
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB
Purity
Affinity purification
Pricing
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Cyclin Dependent Kinase 9 (CDK9), Polyclonal Antibody (Cat# AAA2043099)
Full Name
APC-Linked Polyclonal Antibody to Cyclin Dependent Kinase 9 (CDK9)
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human
Applications
WB, ICC, IHC, ELISA
Pricing
$240/0.1 mL | $310/0.2 mL | $590/0.5 mL | $720/1 mL | $2,030/5x1 mL
Western Blot (WB)
(Host: MouseTarget Name: LATS1Sample Tissue: Mouse PancreasAntibody Dilution: 1ug/ml)
LATS1, Polyclonal Antibody (Cat# AAA3213827)
Full Name
LATS1 antibody - middle region
Gene Names
LATS1; wts; WARTS
Reactivity
Cow, Dog, Horse, Human, Mouse, Rabbit, Rat, Sheep, Yeast, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
CRKRS, Monoclonal Antibody (Cat# AAA6237750)
Full Name
CRKRS (Cdc2-Related Kinase, Arginine/Serine-Rich, CRK7, CRKR, KIAA0904) (MaxLight 750)
Gene Names
CDK12; CRK7; CRKR; CRKRS
Applications
IF, WB
Purity
Purified
Pricing
$795/0.1 mL | $3,570/5x0.1 mL
Western Blot (WB)
(WB Suggested Anti-PIK3R4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate)
PIK3R4, Polyclonal Antibody (Cat# AAA3212048)
Full Name
PIK3R4 antibody - N-terminal region
Gene Names
PIK3R4; p150; VPS15
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 288.9 kDa MTOR protein using Rabbit Anti-MTOR Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-MTOR Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 288.9 kDa. Other Band(s): 25 kDa.)
mTOR, Polyclonal Antibody (Cat# AAA8001797)
Full Name
mTOR Antibody: ATTO 488
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of CDK4 expression in Hela cell lysate (MBS179152).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK4 )
CDK4, Monoclonal Antibody (Cat# AAA179152)
Full Name
Anti-CDK4 Rabbit Monoclonal Antibody
Gene Names
CDK4; CMM3; PSK-J3
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CDK4 (Phospho-Thr172) using serum treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
CDK4, Polyclonal Antibody (Cat# AAA9614008)
Full Name
Phospho-CDK4 (Thr172) Antibody
Gene Names
CDK4; CMM3; PSK-J3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (85%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (85%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (92%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
PRKCE / Protein Kinase C Epsilon Type, Recombinant Protein (Cat# AAA2903546)
Full Name
Human PRKCE / Protein Kinase C Epsilon Type Recombinant Protein
Gene Names
PRKCE; PKCE; nPKC-epsilon
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Chk1, ELISA Kit (Cat# AAA9500118)
Full Name
Chk1 Colorimetric Cell-Based ELISA Kit
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Pricing
$470/1x96 Wells | $2,110/5x96 Wells | $4,150/10x96 Wells
Testing Data
()
Chk1, Polyclonal Antibody (Cat# AAA5310047)
Full Name
Chk1 antibody
Gene Names
CHEK1; CHK1
Applications
WB
Purity
Purified by Protein A affinity chromatography
Western Blot (WB)
(Western blot analysis of lysates from NIH/3T3, HT-29, and RAW264.7 cells, using p50 CDC37 Antibody. The lane on the right is blocked with the synthesized peptide.)
p50 CDC37, Polyclonal Antibody (Cat# AAA9505674)
Full Name
p50 CDC37 Antibody
Gene Names
CDC37; P50CDC37
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)
Cyclin Dependent Kinase 1 (CDK1), ELISA Kit (Cat# AAA2703855)
Full Name
Human Cyclin Dependent Kinase 1 (CDK1) ELISA Kit
Gene Names
Cdk1; cdc2
Reactivity
Human
Pricing
$310/48-Strip-Wells | $385/96-Strip-Wells | $1,545/5x96-Strip-Wells | $2,970/10x96-Strip-Wells | $235/24-Strip-Wells
ELISA (EIA)
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Chk1 (Phospho-Ser296) Antibody)
Chk1, Polyclonal Antibody (Cat# AAA9508582)
Full Name
Chk1 (Phospho-Ser296) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Aurora A expression in MCF7 (A); SP2/0 (B); H9C2 (C) whole cell lysates.)
Aurora A, Polyclonal Antibody (Cat# AAA5316262)
Full Name
Aurora A Antibody
Gene Names
aurA; 87A7-9/2; AAK; aur; Aur; AUR; aur-A; Aur-A; AurA; aurka; AURKA; CG3068; ck10; ck[10]; DmelCG3068; l(3)87Ac; l(3)ck10
Applications
WB, IHC, IF, ICC
Purity
Immunogen Affinity Chromatography
Pricing
$335/0.1 mL | $1,510/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Mouse Liver showing detection of ~16.5, 11.2 kDa CDKN2A protein using Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16.5, 11.2 kDa.)
CDKN2A, Polyclonal Antibody (Cat# AAA8001383)
Full Name
CDKN2A Antibody: ATTO 488
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 288.9 kDa MTOR protein using Rabbit Anti-MTOR Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-MTOR Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 288.9 kDa. Other Band(s): 25 kDa.)
mTOR, Polyclonal Antibody (Cat# AAA8001807)
Full Name
mTOR Antibody: FITC
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Cdk9, Monoclonal Antibody (Cat# AAA8534188)
Full Name
Cdk9
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human
Applications
WB, ICC, IF, IHC, IP
Purity
ProA affinity purified
Pricing
$350/0.1 mL | $570/0.1 mL (Biotin) | $1,515/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
CDK12, Polyclonal Antibody (Cat# AAA8530926)
Full Name
CDK12 Polyclonal Antibody
Gene Names
CDK12; CRK7; CRKR; CRKRS
Reactivity
Human, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of CDC2 (pY15) expression in HeLa UV-treated (A); A431 (B) whole cell lysates.)
CDC2, Polyclonal Antibody (Cat# AAA5316100)
Full Name
CDC2 Antibody
Applications
WB
Purity
Immunogen Affinity Chromatography
Pricing
$395/0.1 mL | $1,765/5x0.1 mL
Testing Data
(Western Blot analysis of extracts from HepG2 cells untreated or treated with UV using Chk1 (Phospho-Ser280) antibody)
Chk1, Polyclonal Antibody (Cat# AAA5309541)
Full Name
Chk1 antibody
Gene Names
CHEK1; CHK1
Applications
WB
Purity
Purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Western Blot (WB)
(Lanes:1: 40ug mouse heart lysate, 2: 40ug mouse heart lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:10000Gene Name:CDK3Submitted by:Anonymous)
CDK3, Polyclonal Antibody (Cat# AAA3224368)
Full Name
CDK3 antibody - C-terminal region
Reactivity
Cow, Dog, Human, Mouse, Rabbit
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using Chk1 Antibody. The picture on the right is blocked with the synthesized peptide.)
Chk1, Polyclonal Antibody (Cat# AAA9503823)
Full Name
Chk1 Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB, IF, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
AKT1 / RAC-alpha Serine/Threonine-Protein Kinase, Recombinant Protein (Cat# AAA2901776)
Full Name
Human AKT1 / RAC-alpha Serine/Threonine-Protein Kinase Recombinant Protein
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 288.9 kDa MTOR protein using Rabbit Anti-MTOR Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-MTOR Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 288.9 kDa. Other Band(s): 25 kDa.)
mTOR, Polyclonal Antibody (Cat# AAA8001804)
Full Name
mTOR Antibody: Alkaline Phosphatase
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of CDK6 expression in HeLa cell lysate (MBS179285).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK6 )
CDK6, Monoclonal Antibody (Cat# AAA179285)
Full Name
Anti-CDK6 Rabbit Monoclonal Antibody
Gene Names
CDK6; MCPH12; PLSTIRE
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Host: RabbitTarget Name: MAP2K3Sample Tissue: Human Neurofibroma Tumor lysatesAntibody Dilution: 1ug/ml)
MAP2K3, Polyclonal Antibody (Cat# AAA3222396)
Full Name
MAP2K3 Antibody - middle region
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human
Applications
WB
Purity
Affinity purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from JurKat cells, using Chk1 Antibody. The lane on the right is blocked with the synthesized peptide.)
Chk1, Polyclonal Antibody (Cat# AAA9504092)
Full Name
Chk1 Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
SIK1 / Serine/Threonine-Protein Kinase SIK1, Recombinant Protein (Cat# AAA2902551)
Full Name
Human SIK1 / Serine/Threonine-Protein Kinase SIK1 Recombinant Protein
Gene Names
SIK1; MSK; SIK; SIK-1; SIK1B; SNF1LK
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 288.9 kDa MTOR protein using Rabbit Anti-MTOR Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-MTOR Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 288.9 kDa. Other Band(s): 25 kDa.)
mTOR, Polyclonal Antibody (Cat# AAA8001806)
Full Name
mTOR Antibody: Biotin
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Chk1 expression in HeLa cell lysate (MBS179539).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHEK1 )
Chk1, Monoclonal Antibody (Cat# AAA179539)
Full Name
Anti-Chk1 Rabbit Monoclonal Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using CDK1 Antibody(MBS179189)CDK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK1 Antibody (MBS179189)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
CDK1/Cdc2, Monoclonal Antibody (Cat# AAA179189)
Full Name
Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Chk1, Monoclonal Antibody (Cat# AAA8533908)
Full Name
Chk1
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$350/0.1 mL | $570/0.1 mL (Biotin) | $1,515/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Typical Testing Data/Standard Curve (for reference only)
Cyclin-dependent kinase 20 (CCRK), ELISA Kit (Cat# AAA167236)
Full Name
Human Cyclin-dependent kinase 20 (CCRK) ELISA Kit
Gene Names
Cdk20; p42; CDCH; Ccrk; PNQLARE; AU019450; AW558027; 4932702G04Rik
Reactivity
Human
Pricing
$285/48-Strip-Wells | $425/96-Strip-Wells | $1,750/5x96-Strip-Wells | $3,460/10x96-Strip-Wells
CHEK1-S317, Polyclonal Antibody (Cat# AAA8536970)
Full Name
Phospho-CHEK1-S317
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB, IF
Purity
Affinity purification
Pricing
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
CDK9, Blocking Peptide (Cat# AAA428858)
Full Name
CDK9 Immunizing Peptide
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Mouse
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Pricing
$215/0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CDK9 antibody.)
CDK9, Polyclonal Antibody (Cat# AAA2554416)
Full Name
CDK9 Polyclonal Antibody
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using CDK6 antibody)
CDK6, Polyclonal Antibody (Cat# AAA5309845)
Full Name
CDK6 antibody
Gene Names
CDK6; MCPH12; PLSTIRE
Applications
IF, IHC
Purity
Purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Western Blot (WB)
(WB Suggested Anti-MAPK4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human heart)
MAPK4, Polyclonal Antibody (Cat# AAA3212768)
Full Name
MAPK4 antibody - middle region
Gene Names
MAPK4; ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using CDK6 (Phospho-Tyr13) antibody)
CDK6, Polyclonal Antibody (Cat# AAA5309634)
Full Name
CDK6 antibody
Gene Names
CDK6; MCPH12; PLSTIRE
Applications
IF, IHC
Purity
Purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using CDK6 (Phospho-Tyr24) antibody)
CDK6, Polyclonal Antibody (Cat# AAA5309635)
Full Name
CDK6 antibody
Gene Names
CDK6; MCPH12; PLSTIRE
Applications
IF, IHC, WB
Purity
Purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Immunohistochemistry (IHC)
((0.5ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
CDK9, Antibody (Cat# AAA423819)
Full Name
Goat anti-CDK9 Antibody
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Mouse
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Liver showing detection of ~16.5, 11.2 kDa CDKN2A protein using Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16.5, 11.2 kDa.)
CDKN2A, Polyclonal Antibody (Cat# AAA8001385)
Full Name
CDKN2A Antibody: ATTO 594
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Liver showing detection of ~16.5, 11.2 kDa CDKN2A protein using Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CDKN2A Polyclonal Antibody (SPC-775) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16.5, 11.2 kDa.)
CDKN2A, Polyclonal Antibody (Cat# AAA8001382)
Full Name
CDKN2A Antibody: ATTO 390
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 288.9 kDa MTOR protein using Rabbit Anti-MTOR Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-MTOR Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 288.9 kDa. Other Band(s): 25 kDa.)
mTOR, Polyclonal Antibody (Cat# AAA8001796)
Full Name
mTOR Antibody: ATTO 390
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
