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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using KIFC3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Rabbit anti-Human, Rat KIFC3 Polyclonal Antibody | anti-KIFC3 antibody

KIFC3 Polyclonal Antibody

Reactivity
Human, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Affinity purification
Synonyms
KIFC3; Polyclonal Antibody; KIFC3 Polyclonal Antibody; anti-KIFC3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
687
Applicable Applications for anti-KIFC3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:200 - 1:2000, IP: 1:20 - 1:50
Immunogen
Recombinant protein of human KIFC3
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using KIFC3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using KIFC3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Related Product Information for anti-KIFC3 antibody
KIFC3 belongs to the large superfamily of kinesins, molecular motors that use the energy of ATP hydrolysis to translocate cargoes along microtubules. Members share extensive homology within a globular domain containing the microtubule- and ATP-binding sites and have a coiled-coil stalk domain that mediates oligomerization. Different kinesin family members participate in specific and diverse motile processes, such as cell division, organelle transport, and nuclear movement (Hoang et al., 1998 [PubMed 9782090]).[supplied by OMIM, Mar 2009]
Product Categories/Family for anti-KIFC3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: 93kDa
Molecular Weight: 833
NCBI Official Full Name
kinesin-like protein KIFC3 isoform 3
NCBI Official Synonym Full Names
kinesin family member C3
NCBI Official Symbol
KIFC3
NCBI Protein Information
kinesin-like protein KIFC3
UniProt Protein Name
Kinesin-like protein KIFC3
Protein Family
UniProt Gene Name
KIFC3
UniProt Entry Name
KIFC3_HUMAN

NCBI Description

This gene encodes a member of the kinesin-14 family of microtubule motors. Members of this family play a role in the formation, maintenance and remodeling of the bipolar mitotic spindle. The protein encoded by this gene has cytoplasmic functions in the interphase cells. It may also be involved in the final stages of cytokinesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

KIFC3: Minus-end microtubule-dependent motor protein. Involved in apically targeted transport. Required for zonula adherens maintenance. Belongs to the kinesin-like protein family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 16q13-q21

Cellular Component: centrosome; cytoplasmic vesicle membrane; Golgi apparatus; kinesin complex; microtubule; zonula adherens

Molecular Function: ATP binding; ATPase activity; microtubule binding; microtubule motor activity; protein binding

Biological Process: Golgi organization and biogenesis; metabolic process; microtubule-based movement; visual perception; zonula adherens maintenance

Research Articles on KIFC3

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Product Notes

The KIFC3 kifc3 (Catalog #AAA9129085) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KIFC3 Polyclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KIFC3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:200 - 1:2000, IP: 1:20 - 1:50. Researchers should empirically determine the suitability of the KIFC3 kifc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIFC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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