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Application
Clone
10000 results for "Biotin Antibody" - showing 900-950
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
Hsp60, Monoclonal Antibody (Cat# AAA803183)
Full Name
Hsp60 Antibody
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Spinach, E.coli (GroEl), H. pylori, S. typhimurium, T. spiralis, Yeast, White Fly
Applications
WB, IHC, FC/FACS
Pricing
$250/0.05 mg | $400/0.2 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Malondialdehyde, Monoclonal Antibody (Cat# AAA808783)
Full Name
Malondialdehyde Antibody, Clone 11E3: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of FABP2/I-FABP using anti- FABP2/I-FABP antibody (MBS1750763).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse small intestine tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- FABP2/I-FABP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FABP2/I-FABP at approximately 15KD. The expected band size for FABP2/I-FABP is at 15KD. )
FABP2/I-FABP, Polyclonal Antibody (Cat# AAA1750763)
Full Name
Anti-FABP2/I-FABP Picoband Antibody
Gene Names
Fabp2; Fabpi; I-FABP
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of SMN1/2 using anti-SMN1/2 antibody (M03420-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: rat RH35 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SMN1/2 antigen affinity purified monoclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.)
SMN1/2, Monoclonal Antibody (Cat# AAA1752271)
Full Name
Anti-SMN1/2 Antibody (monoclonal, 3D10)
Gene Names
SMN1; SMA; SMN; SMA1; SMA2; SMA3; SMA4; SMA@; SMNT; BCD541; GEMIN1; TDRD16A; T-BCD541
Reactivity
Human
Applications
WB, IHC, ICC
Purity
Immunogen Affinity Purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PIK3CB using anti- PIK3CB antibody (MBS1750564).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: mouse spleen tissue lysates,Lane 4: mouse thymus tissue lysates,Lane 5: MCF-7 whole Cell lysates,Lane 6: K562 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PIK3CB antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIK3CB at approximately 110KD. The expected band size for PIK3CB is at 123KD. )
PIK3CB/Pi 3 Kinase P110 Beta, Polyclonal Antibody (Cat# AAA1750564)
Full Name
Anti-PIK3CB/Pi 3 Kinase P110 Beta Picoband Antibody
Gene Names
PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of RPS6 using anti- RPS6 antibody (MBS1750670).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat testis tissue lysates,Lane 2: mouse testis tissue lysates,Lane 3: MCF-7 whole Cell lysates,Lane 4: A549 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- RPS6 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPS6 at approximately 32KD. The expected band size for RPS6 is at 29KD. )
RPS6/Ribosomal Protein S6, Polyclonal Antibody (Cat# AAA1750670)
Full Name
Anti-RPS6/Ribosomal Protein S6 Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of TIMP4 using anti- TIMP4 antibody (MBS1750919).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysates,Lane 2: mouse heart tissue lysates,Lane 3: HELA whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- TIMP4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TIMP4 at approximately 25KD. The expected band size for TIMP4 is at 25KD. )
TIMP4, Polyclonal Antibody (Cat# AAA1750919)
Full Name
Anti-TIMP4 Picoband Antibody
Gene Names
TIMP4; TIMP-4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
CD279/PD-1, Monoclonal Antibody (Cat# AAA2556974)
Full Name
Anti-Mouse CD279/PD-1 Monoclonal Antibody (Biotin Conjugated)
Gene Names
Pdcd1; PD-1; Pdc1; Ly101
Reactivity
Mouse
Applications
FC/FACS
Pricing
$170/0.1 mg | $115/0.025 mg | $745/5x0.1 mg
Testing Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD32: Biotin (MBS210308))
CD32, Monoclonal Antibody (Cat# AAA214770)
Full Name
MOUSE ANTI HUMAN CD32:Biotin
Gene Names
FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Applications
FC/FACS
Pricing
$195/0.025 mg | $730/5x0.025 mg
CD35, Monoclonal Antibody (Cat# AAA2553848)
Full Name
Anti-Human CD35 Monoclonal Antibody (Biotin Conjugated)
Reactivity
Human
Applications
FC/FACS
Pricing
$170/0.1 mg | $100/0.025 mg | $755/5x0.1 mg
CD71, Monoclonal Antibody (Cat# AAA2553898)
Full Name
Anti-Mouse CD71 Monoclonal Antibody (Biotin Conjugated)
Reactivity
Mouse
Applications
FC/FACS
Pricing
$105/0.05 mg | $125/0.1 mg | $545/5x0.1 mg
Testing Data
(Published customer image: Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. (A) Log forward and side scatter chart of platelet population (gated) with minimal contaminating cells. (B) Fluorescence chart of CD45 (FL2) and CD61 (FL1) antibody-labeled samples. Average CD61+: 99.47?+/-?0.21% (n?=?3). Average CD45+: 0.19?+/-?0.04% (n?=?3). (C) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C. Ultra-pure platelet isolation from canine whole blood. BMC Vet Res. 2013 Jul 17;9:144.)
CD45, Monoclonal Antibody (Cat# AAA214756)
Full Name
RAT ANTI DOG CD45:Biotin
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Applications
FC/FACS
Pricing
$560/0.1 mg | $2,365/5x0.1 mg
CD10, Monoclonal Antibody (Cat# AAA2553868)
Full Name
Anti-Human CD10 Monoclonal Antibody (Biotin Conjugated)
Gene Names
MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
Reactivity
Human
Applications
FC/FACS
Pricing
$170/0.1 mg | $100/0.025 mg | $755/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Tissue: breast carcinoma. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:100.)
PUMA (NT), Polyclonal Antibody (Cat# AAA806760)
Full Name
PUMA (NT) Antibody: HRP
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human
Applications
WB, IHC
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD7:Biotin (MBS210314))
CD7, Monoclonal Antibody (Cat# AAA212053)
Full Name
MOUSE ANTI HUMAN CD7
Gene Names
CD7; GP40; TP41; Tp40; LEU-9
Applications
FC/FACS, IP
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Testing Data
(Staining of CD209 transfected K562 cells with Mouse anti Human CD209: Alexa Fluor 647 (MBS215905A647))
CD209, Monoclonal Antibody (Cat# AAA215910)
Full Name
MOUSE ANTI HUMAN CD209:FITC
Gene Names
CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215790)
Full Name
RAT ANTI MOUSE DECTIN-1:Biotin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46. Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
Cav1.2, Monoclonal Antibody (Cat# AAA800295)
Full Name
CaV1.2 Antibody: Biotin
Gene Names
CACNA1C; CACB
Reactivity
Human, Mouse, Rat
Applications
WB, IP, IHC, IF
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6. Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)
KCNQ4, Monoclonal Antibody (Cat# AAA801510)
Full Name
KCNQ4 Antibody: Biotin
Gene Names
KCNQ4; DFNA2; KV7.4; DFNA2A
Reactivity
Human, Mouse, Rat
Applications
WB, IP, ICC, IF
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)
Hsc70 (Hsp73), Polyclonal Antibody (Cat# AAA805504)
Full Name
Hsc70 (Hsp73) Antibody
Gene Names
HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
Reactivity
Human, Hamster, Rat
Applications
WB, IP, IF, IHC
Pricing
$395/0.1 mL | $1,535/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TRAP1 Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)
TRAP1, Polyclonal Antibody (Cat# AAA806361)
Full Name
TRAP1 Antibody: Biotin
Gene Names
TRAP1; HSP75; HSP 75; HSP90L; TRAP-1
Reactivity
Human, Rat, Mouse
Applications
WB
Pricing
$450/0.1 mg | $1,805/5x0.1 mg
Testing Data #1
(Staining of JAM-C transfected CHO cells with Rat anti Mouse JAM-C:FITC (MBS224678))
JAM-C, Monoclonal Antibody (Cat# AAA224659)
Full Name
RAT ANTI MOUSE JAM-C:Biotin
Gene Names
Jam3; JAM-3; JAM-C; Jcam3; 1110002N23Rik
Applications
FC/FACS, IF, IP
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:5000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
Hsp90 alpha, Monoclonal Antibody (Cat# AAA803265)
Full Name
Hsp90 alpha Antibody: HRP
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
Reactivity
Human (Alpha specific), Rat
Applications
WB, EIA, IHC, IP
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:5000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
Hsp90 alpha, Monoclonal Antibody (Cat# AAA802045)
Full Name
Hsp90 alpha Antibody
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
Reactivity
Human (Alpha specific), Rat
Applications
WB, EIA, IHC, IP
Pricing
$275/0.025 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)
alpha B Crystallin, Polyclonal Antibody (Cat# AAA805750)
Full Name
Alpha B Crystallin Antibody: Biotin
Gene Names
CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
Reactivity
Human, Mouse, Rat, Cow, Chicken
Applications
WB, IHC, ICC
Pricing
$470/0.2 mL | $1,885/5x0.2 mL
Testing Data
(Staining of mouse spleen cells with Mouse anti Rat CD71:Low Endotoxin (MBS211202))
CD71, Monoclonal Antibody (Cat# AAA212213)
Full Name
MOUSE ANTI RAT CD71
Gene Names
Tfrc; Trfr
Applications
FC/FACS, IF, WB
Pricing
$490/0.25 mg | $2,050/5x0.25 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808603)
Full Name
Acrolein Antibody, Clone 10A10: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of TMEM107 using anti- TMEM107 antibody (MBS1750887).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: MCF-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- TMEM107 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TMEM107 at approximately 22KD. The expected band size for TMEM107 is at 16KD. )
TMEM107, Polyclonal Antibody (Cat# AAA1750887)
Full Name
Anti-TMEM107 Picoband Antibody
Gene Names
TMEM107; MKS13; JBTS29; GRVS638; PRO1268
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at-20 degree C. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
HSP90, Monoclonal Antibody (Cat# AAA8001899)
Full Name
HSP90 (Total) Antibody
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human, Mouse, Rat, Plant, Wheat
Applications
WB, IHC, ICC, IF, IP, EIA
Purity
Protein G Purified
Pricing
$275/0.05 mg | $990/5x0.05 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PLK2/Snk using anti- PLK2/Snk antibody (MBS176157). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A431 Cell Lysate Lane 2: 293T Cell Lysate Lane 3: COLO-320 Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PLK2/Snk antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLK2/Snk at approximately 78KD. The expected band size for PLK2/Snk is at 77KD. )
PLK2, Polyclonal Antibody (Cat# AAA176157)
Full Name
Anti-PLK2 antibody
Gene Names
PLK2; SNK; hSNK; hPlk2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of TNFSF18 using anti-TNFSF18 antibody (MBS1750870).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human TNFSF18 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFSF18 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNFSF18 at approximately 15KD. The expected band size for TNFSF18 is at 15KD. )
TNFSF18/Gitrl, Polyclonal Antibody (Cat# AAA1750870)
Full Name
Anti-TNFSF18/Gitrl Picoband Antibody
Gene Names
TNFSF18; TL6; AITRL; GITRL; TNLG2A; hGITRL
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CPI17 alpha using anti- CPI17 alpha antibody (MBS1750904).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates,Lane 3: PANC-1 whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CPI17 alpha antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CPI17 alpha at approximately 20KD. The expected band size for CPI17 alpha is at 20KD. )
CPI17 alpha, Polyclonal Antibody (Cat# AAA1750904)
Full Name
Anti-CPI17 alpha Picoband Antibody
Gene Names
PPP1R14A; CPI17; CPI-17; PPP1INL
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Prothrombin using anti-Prothrombin antibody (MBS1750316).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human plasma lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prothrombin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Prothrombin at approximately 85KD. The expected band size for Prothrombin is at 70KD. )
Prothrombin, Polyclonal Antibody (Cat# AAA1750316)
Full Name
Anti-Prothrombin Picoband antibody
Gene Names
F2; PT; THPH1; RPRGL2
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of ErbB 2 using anti- ErbB 2 antibody (MBS1750304).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ErbB 2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ErbB 2 at approximately 138KD. The expected band size for ErbB 2 is at 138KD. )
ErbB 2, Polyclonal Antibody (Cat# AAA1750304)
Full Name
Anti-ErbB 2 Picoband Antibody
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (MBS1750347).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt3 / CD135 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Flt3 / CD135 at approximately 160KD. The expected band size for Flt3 / CD135 is at 113KD. )
Flt3/CD135, Polyclonal Antibody (Cat# AAA1750347)
Full Name
Anti-Flt3/CD135 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
CD47, Monoclonal Antibody (Cat# AAA2553844)
Full Name
Anti-Human CD47 Monoclonal Antibody (Biotin Conjugated)
Reactivity
Human
Applications
FC/FACS
Pricing
$190/0.1 mg | $105/0.025 mg | $840/5x0.1 mg
CD62L, Monoclonal Antibody (Cat# AAA2553764)
Full Name
Anti-Mouse CD62L Monoclonal Antibody (Biotin Conjugated)
Gene Names
Sell; Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin
Reactivity
Mouse
Applications
FC/FACS
Pricing
$130/0.05 mg | $155/0.1 mg | $700/5x0.1 mg
Testing Data
(Staining of human peripheral blood platelets with Mouse anti Human CD49b:RPE (MBS213872))
CD49b, Monoclonal Antibody (Cat# AAA210513)
Full Name
MOUSE ANTI HUMAN CD49b:Biotin
Gene Names
ITGA2; BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD32: Biotin (MBS210308))
CD32, Monoclonal Antibody (Cat# AAA214772)
Full Name
MOUSE ANTI HUMAN CD32:RPE
Gene Names
FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Applications
FC/FACS
Pricing
$215/25 Tests | $825/5x25 Tests
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD16:Biotin(MBS210312))
CD16, Monoclonal Antibody (Cat# AAA214829)
Full Name
MOUSE ANTI HUMAN CD16:FITC
Gene Names
FCGR3B; CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
Applications
FC/FACS
Pricing
$195/0.025 mg | $730/5x0.025 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)
Hsp90, Monoclonal Antibody (Cat# AAA803070)
Full Name
Hsp90 Antibody: ATTO 594
Reactivity
Bovine, Chicken, Human, Mouse, Porcine (Pig), Rat, Rabbit
Applications
IP, IHC, WB
Pricing
$495/0.1 mg | $2,000/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of AGRP using anti-AGRP antibody (MBS1750364).AGRP was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AGRP Antibody (MBS1750364) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
AGRP/Art, Polyclonal Antibody (Cat# AAA1750364)
Full Name
Anti-AGRP/Art Picoband antibody
Gene Names
Agrp; Art; Agrt
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Lanes:Rat brain sectionPrimary Antibody Dilution:1:500Secondary Antibody:Anti-rabbit-biotin, streptavidin-diaminobenzidineSecondary Antibody Dilution:1:500Gene Name:KCNN2Submitted by:Dr. Amiel Rosenkranz, Rosalind Franklin University)
KCNN2, Polyclonal Antibody (Cat# AAA3202426)
Full Name
KCNN2 antibody - C-terminal region
Gene Names
KCNN2; SK2; hSK2; SKCA2; KCa2.2; SKCa 2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish, Monkey
Applications
IHC, WB
Purity
Protein A purified
Pricing
$390/0.1 mL | $1,750/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12. Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.)
p38 alpha, Monoclonal Antibody (Cat# AAA803333)
Full Name
p38 alpha (MAP Kinase) Antibody: Biotin
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Rat
Applications
WB, IP, EIA
Pricing
$410/0.1 mg | $1,625/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2 . Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
HSP60, Monoclonal Antibody (Cat# AAA8001869)
Full Name
HSP60 Antibody
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Plant, Spinach, Bacteria, E Coli, Helicobacter pylori, Salmonella Typhimurium, Trichinella Spiralis, Yeast, Insect, White Fly, Fish, Three-spined stickleback
Applications
WB, IHC, FC/FACS
Purity
Protein G Purified
Pricing
$250/0.05 mg | $905/5x0.05 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808819)
Full Name
Methylglyoxal Antibody, Clone 9F11: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005625)
Full Name
VPS35 Antibody, Clone 5A9: Biotin
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$535/0.1 mg | $2,170/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PDPK1 using anti-PDPK1 antibody (MBS177711).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Liver Tissue Lysate,Lane 2: Rat Lung Tissue Lysate,Lane 3: Mouse Liver Tissue Lysate,Lane 4: Mouse Lung Tissue Lysate,Lane 5: COLO320 Whole Cell Lysate,Lane 6: MCF-7 Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDPK1 at approximately 70KD. The expected band size for PDPK1 is at 63KD. )
PDPK1, Polyclonal Antibody (Cat# AAA177711)
Full Name
Anti-PDPK1 Antibody
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of DDB1 using anti-DDB1 antibody (MBS178141).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Brain Tissue Lysate,Lane 2: Rat Liver Tissue Lysate,Lane 3: Mouse Ovary Tissue Lysate,Lane 4: Mouse Testis Tissue Lysate,Lane 5: MCF-7 Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DDB1 at approximately 127KD. The expected band size for DDB1 is at 127KD. )
DDB1, Polyclonal Antibody (Cat# AAA178141)
Full Name
Anti-DDB1 Antibody
Gene Names
DDB1; XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Bid using anti-Bid antibody (MBS1750470).
Bid was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bid Antibody (MBS1750470) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Bid, Polyclonal Antibody (Cat# AAA1750470)
Full Name
Anti-Bid Picoband Antibody
Gene Names
Bid; AI875481; AU022477; 2700049M22Rik
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
