Mouse anti-Human CD47 Monoclonal Antibody | anti-CD47 antibody
Anti-Human CD47 Monoclonal Antibody (Biotin Conjugated)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD47; Monoclonal Antibody; Anti-Human CD47 Monoclonal Antibody (Biotin Conjugated); Human CD47; Leukocyte surface antigen CD47; Cd47; Integrin-associated protein; IAP; anti-CD47 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CC2C6D4
Form/Format
Liquid; PBS with 0.05% Proclin300, 1% BSA
Sequence Length
292
Applicable Applications for anti-CD47 antibody
Flow Cytometry (FC/FACS)
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <=0.5 ug per 10e6 cells in 100 ul volume or 100 ul of whole blood.
Conjugation
Biotin
Cellular Localization
Membrane
Preparation and Storage
Store at 2-8 degree C. Protected from prolonged exposure to light.
Related Product Information for anti-CD47 antibody
CD47 also known as Rh-associated protein, gp42, integrin-associated protein (IAP), and neurophilin, is a 42-52 kD member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. Two splice variants have been described in the cytoplasmic tail, the shorter form is expressed in bone-marrow-derived cells, endothelial cells, and fibroblasts while the longer form is expressed by neural tissues. CD47 expression is widely distributed in hematopoietic cells including thymocytes, T cells, B cells, monocytes, platelets, and erythrocytes as well as epithelial cells, endothelial cells, fibroblasts, and neural tissues. CD47 functions as an adhesion molecule and thrombospondin receptor and is non-covalently associated with beta3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47; in the absence of CD47 mice show defects in host defense and beta3 integrin-dependent ligand binding, migration, and cellular activation. CD47 is also part of the Rh complex on erythrocytes.
References
1. Anstee DJ, et al. 1995. In Leucocyte Typing V (Schlossman ed.) Oxford University Press Oxford pp233-234. 2. Brown E, et al. 1990. J. Cell Biol. 111:2785. 3. Gao AG, et al. 1996. J. Biol. Chem. 271:21. 4. Lindberg FP, et al. 1994. J. Biol. Chem. 269:1567.
NCBI and Uniprot Product Information
Uniprot Description
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
Similar Products
Product Notes
The CD47 cd47 (Catalog #AAA2553844) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human CD47 Monoclonal Antibody (Biotin Conjugated) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD47 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <=0.5 ug per 10e6 cells in 100 ul volume or 100 ul of whole blood. Researchers should empirically determine the suitability of the CD47 cd47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD47, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
