Rabbit TRAP1 Polyclonal Antibody | anti-TRAP1 antibody

TRAP1 Antibody: Biotin

Cat. Num. AAA806361

• Gene Names: TRAP1; HSP75; HSP 75; HSP90L; TRAP-1
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Synonyms: TRAP1; Polyclonal Antibody; TRAP1 Antibody: Biotin; Heat shock protein 75; Heat shock protein 75 kDa; Heat shock protein 75 kDa mitochondrial; HSP 75; HSP 90L; HSP75; HSP90L; mitochondrial; TNF receptor associated protein 1; TNFR associated protein 1; TNFR-associated protein 1; TRAP 1; TRAP-1; Trap1; anti-TRAP1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Form/Format: Protein A Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 704

• Applicable Applications for anti-TRAP1 antibody: Western Blot (WB)
• Application Notes: 1:1000 (WB)
• Conjugate: Biotin
• Storage Buffer: PBS pH 7.4, 0.09% azide, 50% glycerol.
• Preparation and Storage: -20 degree C

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TRAP1 Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Western Blot (WB)

(Western blot analysis of Human Cervical Cancer cell lysates (HeLa) showing detection of ~75 kDa TRAP1 protein using Rabbit Anti-TRAP1 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical Cancer cell lysates (HeLa). Load: 12 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~75 kDa. Other Band(s): 100 kDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TRAP1 Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Related Product Information for anti-TRAP1 antibody

Background Info: Detects TRAP1. Detects ~75 kDa, multiple isoforms.

Scientific Background: The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like hsp90, TRAP1 is found to beassociated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).

Product Categories/Family for anti-TRAP1 antibody

Heat Shock

References

1. Felts S.J., et al. (2000) J Biol Chem. 275(5): 3305-3312. 2. Costantino E., et al. (2009) Cancer Lett. 279(1): 39-46. 3. Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.

NCBI and Uniprot Product Information

• NCBI GI #: 155722983
• NCBI GeneID: 10131
• NCBI Accession #: NP_057376.2
• NCBI GenBank Nucleotide #: NM_016292.2
• UniProt Accession #: Q12931; O43642; O75235; Q9UHL5; B4DR68; D3DUC8; F5H897
• Molecular Weight: 74,267 Da
• NCBI Official Full Name: heat shock protein 75 kDa, mitochondrial isoform 1
• NCBI Official Synonym Full Names: TNF receptor-associated protein 1
• NCBI Official Symbol: TRAP1
• NCBI Official Synonym Symbols: HSP75; HSP 75; HSP90L; TRAP-1
• NCBI Protein Information: heat shock protein 75 kDa, mitochondrial; TNFR-associated protein 1; tumor necrosis factor type 1 receptor-associated protein
• UniProt Protein Name: Heat shock protein 75 kDa, mitochondrial
• Protein Family: TNF receptor-associated protein
• UniProt Gene Name: TRAP1
• UniProt Synonym Gene Names: HSP75; HSP 75; TRAP-1
• UniProt Entry Name: TRAP1_HUMAN

NCBI Description

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

HSP75: Chaperone that expresses an ATPase activity. Belongs to the heat shock protein 90 family.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; mitochondrion; membrane; mitochondrial matrix; mitochondrial inner membrane; mitochondrial intermembrane space; lipid particle

Molecular Function: protein binding; unfolded protein binding; tumor necrosis factor receptor binding; protein kinase binding; ATP binding

Biological Process: response to stress