Mouse CD71 Monoclonal Antibody | anti-CD71 antibody

MOUSE ANTI RAT CD71

Cat. Num. AAA212213

• Gene Names: Tfrc; Trfr
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: CD71; Monoclonal Antibody; MOUSE ANTI RAT CD71; anti-CD71 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: OX-26
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1 mg/ml (varies by lot)
• Sequence Length: 622

• Applicable Applications for anti-CD71 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology Paraffin, Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of mouse spleen cells with Mouse anti Rat CD71:Low Endotoxin (MBS211202))

Testing Data

(Staining of mouse spleen with Mouse anti Rat CD71:Biotin (MBS210347))

Testing Data

(Staining of rat spleen cells with Mouse anti Rat CD71:RPE (MBS213633))

Testing Data

(Staining of rat spleen lymphocytes with Mouse anti Rat CD71 (MBS212211))

Testing Data

(Published customer image: FITC conjugated Mouse anti Rat CD71 antibody, clone OX-26 used for flow cytometryImage caption:Flow cytometric analysis of follicle stem cells separated by Vario MACS. (a -d) Single-color flow cytometric analysis of the selected follicle stem cells. X axis represents fluorescence intensity, and Y axis represents cell number. (a) Bulge region cell suspension without antibody staining was used as control. (b) CD34bri cells were isolated by Vario MACS. (c) Control for a6 separation. (d) a6bri cells were isolated by Vario MACS. (e -h) Two-color flow cytometric analysis of follicle stem cells selected by Vario MACS. Cells were divided into four subpopulations: a PEbri FITCdim cells; b PEbri FITCbri cells; c PEdim FITCdim cells; and d PEdim FITCbri cells. (e) Control. (f) CD71 and CD34 was detected with FITC (X axis) and with PE (Y axis). The majority of the isolated cells were CD34briCD71dim cells, characterized by high level expression of CD34 and low level of CD71. No CD34dimCD71bri cells were detected in our experiments. (g) Control for a6 and CD71 combined separation; (h) The a6briCD71dim cells were the main subpopulation of the separated cells.From: Huang E, Lian X, Chen W, Yang T, Yang L. Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system. Acta Histochem Cytochem. 2009 Oct 30;42(5):129-36.)

Related Product Information for anti-CD71 antibody

Mouse anti Rat CD71 antibody, clone OX-26 recognizes rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues. The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis (Crihton et al. 1992). An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer's, Parkinson's, Huntington's and Multiple Sclerosis (Benarroch 2009). Mouse anti rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy (Lipardi et al. 2002). OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195kDa under non-reducing conditions reflecting it's homodimeric structure.

NCBI and Uniprot Product Information

• NCBI GI #: 20140355
• NCBI GeneID: 64678
• UniProt Accession #: Q99376
• Molecular Weight: 70,153 Da
• NCBI Official Full Name: Transferrin receptor protein 1
• NCBI Official Synonym Full Names: transferrin receptor
• NCBI Official Symbol: Tfrc
• NCBI Official Synonym Symbols: Trfr
• NCBI Protein Information: transferrin receptor protein 1; TR; tfR; tfR1
• UniProt Protein Name: Transferrin receptor protein 1
• UniProt Gene Name: Tfrc
• UniProt Synonym Gene Names: Trfr; TR; TfR; TfR1; Trfr
• UniProt Entry Name: TFR1_RAT

NCBI Description

receptor for transferrin; involved in regulating stellate cell activation [RGD, Feb 2006]

Uniprot Description

TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.

Protein type: Membrane protein, integral; Cell surface; Receptor, misc.

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; mitochondrion; recycling endosome membrane; cell; cytoplasmic membrane-bound vesicle; integral to membrane; extracellular region; coated pit; recycling endosome; perinuclear region of cytoplasm; melanosome; plasma membrane; vesicle; endosome; external side of plasma membrane

Molecular Function: identical protein binding; transferrin receptor activity; chaperone binding; double-stranded RNA binding; Hsp70 protein binding

Biological Process: receptor-mediated endocytosis; response to inorganic substance; response to retinoic acid; cellular iron ion homeostasis; transferrin transport; osteoclast differentiation; regulation of cell proliferation; response to manganese ion; response to organic substance; response to copper ion; acute-phase response; response to hypoxia; positive regulation of bone resorption; regulation of cell growth; response to iron ion; response to nutrient; aging