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10000 results for " Protein Kinase c" - showing 800-850


Western Blot (WB) (Figure 1. Western blot analysis of PKC eta using anti-PKC eta antibody (MBS178258). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate Lane 2: A549 Whole Cell Lysate Lane 3: HELA Whole Cell Lysate Lane 4: SKOV Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC eta antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PKC eta at approximately 78KD. The expected band size for PKC eta is at 78KD. )

PKC eta, Polyclonal Antibody (Cat# AAA178258)

Full Name
Anti-PKC eta Antibody
Gene Names
PRKCH; PKCL; PKC-L; PRKCL; nPKC-eta
Reactivity
Human
Applications
WB
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MerTK Mouse Monoclonal Antibody (MERTK/3015).)

MerTK, Monoclonal Antibody (Cat# AAA4380783)

Full Name
MerTK (Innate Immune Checkpoint)
Gene Names
MERTK; MER; RP38; c-Eyk; c-mer; Tyro12
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Figure 1. Western blot analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (MBS1750606).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VEGF Receptor 3 at approximately 153KD. The expected band size for VEGF Receptor 3 is at 153KD. )

VEGF Receptor 3, Polyclonal Antibody (Cat# AAA1750606)

Full Name
Anti-VEGF Receptor 3 Picoband antibody
Gene Names
FLT4; PCL; FLT-4; FLT41; LMPH1A; VEGFR3; VEGFR-3
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB, FC
Purity
Immunoggen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts of 293 cells, using Phospho-PKCalpha-S226 antibody at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Phospho-PKCalpha-S226, Antibody (Cat# AAA9128997)

Full Name
Phospho-PKCalpha-S226 Polyclonal Antibody
Gene Names
PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
Reactivity
Human
Applications
WB
Purity
Affinity purification
Pricing
$260/0.05 mL | $385/0.1 mL | $570/0.2 mL
Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: C2C12) using a 10 % SDS PAGE gel and PKA alpha antibody at a dilution of 1:1000)

PKA alpha, Polyclonal Antibody (Cat# AAA835160)

Full Name
PKA alpha antibody
Applications
IF, ICC, WB
Purity
PKA alpha antibody was purified by antigen-affinity chromatography
Pricing
$700/0.1 mL | $3,130/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts of Raji cells, using KIDINS220 antibody.)

KIDINS220, Polyclonal Antibody (Cat# AAA9603643)

Full Name
KIDINS220 Antibody
Gene Names
KIDINS220; ARMS; SINO
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (At 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

OSR1, Polyclonal Antibody (Cat# AAA9610530)

Full Name
Phospho-OSR1 (Ser339) Antibody
Gene Names
OXSR1; OSR1
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken
Applications
WB, IHC, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of Phospho-OSR1 (Thr185) using K562 whole cell lysates)

OSR1, Polyclonal Antibody (Cat# AAA9609059)

Full Name
Phospho-OSR1 (Thr185) Antibody
Gene Names
OXSR1; OSR1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from COLO205 cells, using CDKL1 antibody.)

CDKL1, Polyclonal Antibody (Cat# AAA9604695)

Full Name
CDKL1 Antibody
Gene Names
CDKL1; P42; KKIALRE
Reactivity
Human, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from rat brain cells, using CDK5R1 antibody.)

CDK5R1, Polyclonal Antibody (Cat# AAA9604746)

Full Name
CDK5R1 Antibody
Gene Names
CDK5R1; p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601685 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

JAK2, Polyclonal Antibody (Cat# AAA9601685)

Full Name
JAK2 Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of NEK2 expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)

NEK2, Polyclonal Antibody (Cat# AAA9604183)

Full Name
NEK2 Antibody
Gene Names
NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
Reactivity
Human
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601000 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

p70 S6 Kinase, Polyclonal Antibody (Cat# AAA9601000)

Full Name
Phospho-p70 S6 Kinase (Ser411) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of PI3-kinase p85-alpha/gamma expression in H2O2 treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

PI3-kinase p85-alpha/gamma, Polyclonal Antibody (Cat# AAA9601832)

Full Name
PI3-kinase p85-alpha/gamma Antibody
Gene Names
PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600834 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

C-RAF, Polyclonal Antibody (Cat# AAA9600834)

Full Name
Phospho-C-RAF (Ser621) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)

Akt, Monoclonal Antibody (Cat# AAA435310)

Full Name
Phospho-Akt (Ser473) mAb
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF
Pricing
$405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Immunohistochemistry (IHC) (At 1/100 staining rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

AXL, Polyclonal Antibody (Cat# AAA9610655)

Full Name
Phospho-AXL (Tyr703) Antibody
Gene Names
AXL; ARK; UFO; JTK11; Tyro7
Reactivity
Human,Mouse,Rat
Applications
WB, IHC, EIA
Purity
Peptide affinity purification
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunofluorescence (IF) (MBS9604652 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9604652) and mouse anti-beta tubulin Ab(MBS576566) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)

CLK2, Polyclonal Antibody (Cat# AAA9604652)

Full Name
CLK2 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600283 at 1/100 staining human lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

CIB2, Polyclonal Antibody (Cat# AAA9600283)

Full Name
CIB2 Antibody
Gene Names
CIB2; KIP2; USH1J; DFNB48
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA, IF, ICC
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data

EGFR, Monoclonal Antibody (Cat# AAA670104)

Full Name
Mouse Anti-Human EGFR-UNLB
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human
Applications
Immunohistochemistry; Western Blot
Pricing
$300/0.1 mg | $1,200/5x0.1 mg
Immunohistochemistry (IHC) (MBS9603593 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

CIN85, Polyclonal Antibody (Cat# AAA9603593)

Full Name
CIN85 Antibody
Gene Names
SH3KBP1; HSB1; CIN85; GIG10; HSB-1; MIG18; CD2BP3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.)

14-3-3 zeta, Polyclonal Antibody (Cat# AAA9502319)

Full Name
14-3-3 zeta (Phospho-Ser58) Antibody
Gene Names
YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB) (Western blot analysis of Human Cell line lysates showing detection of ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Erk1/2, Polyclonal Antibody (Cat# AAA805344)

Full Name
Erk1/2 Antibody: Alkaline Phosphatase
Gene Names
Mapk3; p44; ERK1; ERT2; MNK1; Erk-1; Esrk1; MAPK1; Prkm3; p44erk1; p44mapk
Reactivity
Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, Xenopus
Applications
WB, IHC, ICC, IF
Pricing
$425/0.1 mL | $1,680/5x0.1 mL
Western Blot (WB) (Western blot analysis on Jurkat cell lysate using MAST3 Antibody, The lane on the left is treated with the antigen-specific peptide.)

MAST3, Polyclonal Antibody (Cat# AAA9600376)

Full Name
MAST3 Antibody
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data (Elastase 4 (ELA4) is a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. It regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ELA4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ELA4 and recombinant rat uPAR. Briefly, ELA4 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ELA4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of ELA4 and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.)

Elastase 4 (ELA4), Active Protein (Cat# AAA2097293)

Full Name
Active Elastase 4 (ELA4)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $475/0.05 mg | $735/0.1 mg | $910/0.2 mg | $1,775/0.5 mg | $2,625/1 mg | $6,415/5 mg
Immunohistochemistry (IHC) (MBS9600655 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

CaMKII, Polyclonal Antibody (Cat# AAA9600655)

Full Name
Phospho-CaMKII (Tyr231) Antibody
Gene Names
CAMK2B; CAM2; CAMK2; CAMKB; MRD54
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from HepG2, using IKK-gamma (Phospho-Ser85) Antibody.)

IKK-gamma, Polyclonal Antibody (Cat# AAA9601602)

Full Name
IKK-gamma (Phospho-Ser85) Antibody
Gene Names
IKBKG; IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; IKKAP1; ZC2HC9; IKK-gamma
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9601213 at 1/100 staining human Placenta tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

PKD1/2/3/PKC mu, Polyclonal Antibody (Cat# AAA9601213)

Full Name
Phospho-PKD1/2/3/PKC mu (Ser738+Ser742) Antibody
Gene Names
PRKD1; PKD; PKCM; CHDED; PRKCM; PKC-MU
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600822 at 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. )

EGFR, Polyclonal Antibody (Cat# AAA9600822)

Full Name
Phospho-EGFR (Ser695) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9610380 at 1/100 staining rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary Ab.)

MuSK, Polyclonal Antibody (Cat# AAA9610470)

Full Name
Phospho-MuSK (Tyr755) Antibody
Gene Names
MUSK; CMS9; FADS; FADS1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Peptide affinity purification
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Figure 1: Western blot analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.)

GRK2, Monoclonal Antibody (Cat# AAA9602685)

Full Name
GRK2 Antibody
Gene Names
GRK2; BARK1; ADRBK1; BETA-ARK1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
Affinity-Chromatography
Pricing
$260/0.05 mL | $325/0.1 mL | $400/0.2 mL | $1,750/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600808 at 1/100 staining rat colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. )

Chk2, Polyclonal Antibody (Cat# AAA9600808)

Full Name
Phospho-Chk2 (Thr68) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot detection of Akt (pan) in C6, 3T3, COS7 and Hela cell lysates using Akt (pan) mouse mAb(dilution 1:1000).Predicted band size:60kDa.Observed band size:60kDa.)

Akt, Monoclonal Antibody (Cat# AAA477376)

Full Name
Anti-Akt (pan) Mouse mAb
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Western Blot (WB) (Western blot analysis of CPI17 expression in Jurkat (A), HT29 (B) whole cell lysates.)

CPI17, Polyclonal Antibody (Cat# AAA8243004)

Full Name
Anti-CPI17 Antibody
Gene Names
PPP1R14A; CPI17; CPI-17; PPP1INL
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, IHC, IF, ICC
Purity
The antibody was purified by affinity chromatography.
Pricing
$175/0.03 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Western Blot (WB) (Western blot analysis of PIK3CD using COS7 whole lysates.)

PIK3CD, Polyclonal Antibody (Cat# AAA9608216)

Full Name
PIK3CD Antibody
Gene Names
PIK3CD; APDS; PI3K; IMD14; p110D; P110DELTA
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data (Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Estrogen Receptor Alpha (ERa) has been identified as an interactor of, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Arginase( Arg) and recombinant rat ERa. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ERa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and ERa was shown in Figure 1, and this effect was in a dose dependent manner.)

Arginase (ARG), Active Protein (Cat# AAA2097289)

Full Name
Active Arginase (ARG)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$205/0.01 mg | $420/0.05 mg | $640/0.1 mg | $790/0.2 mg | $1,535/0.5 mg | $2,290/1 mg | $5,590/5 mg
Immunohistochemistry (IHC) (MBS9601104 at 1/50 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

GSK3 alpha/beta, Polyclonal Antibody (Cat# AAA9601104)

Full Name
Phospho-GSK3 alpha/beta (Tyr279/216) Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of PI3 kinase P110alpha expression in rat liver)

PI3 kinase P110alpha, Polyclonal Antibody (Cat# AAA9601285)

Full Name
PI3 kinase P110alpha Antibody
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from mouse spleen, using CaMKIIbeta/gamma Antibody.)

CaMKIIbeta/gamma, Polyclonal Antibody (Cat# AAA9602073)

Full Name
CaMKIIbeta/gamma Antibody
Gene Names
CAMK2G; CAMK; CAMKG; CAMK-II
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9608976 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

GAS6, Polyclonal Antibody (Cat# AAA9608976)

Full Name
GAS6 Antibody
Gene Names
GAS6; AXSF; AXLLG
Reactivity
Human
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600795 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

JAK2, Polyclonal Antibody (Cat# AAA9600795)

Full Name
Phospho-JAK2 (Tyr221) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of Phospho-C-RAF (Ser259) expression in various lysates)

C-RAF, Polyclonal Antibody (Cat# AAA9600836)

Full Name
Phospho-C-RAF (Ser259) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601670 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

CHK1, Polyclonal Antibody (Cat# AAA9601670)

Full Name
CHK1 Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H.liver section using MET/HGFR Antibody. MBS9200230 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

MET/HGFR, Monoclonal Antibody (Cat# AAA9200230)

Full Name
MET/HGFR Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human, mouse
Applications
IF, EIA, WB, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunofluorescence (IF) (MBS9601261 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CaMK2 alpha/beta/delta, Polyclonal Antibody (Cat# AAA9601261)

Full Name
Phospho-CaMK2 alpha/beta/delta (Thr305) Antibody
Gene Names
CAMK2A; CAMKA; MRD53; MRT63; CaMKIINalpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9601033 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Akt, Polyclonal Antibody (Cat# AAA9601033)

Full Name
Phospho-Akt (Thr308) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot detection of CDK9 in Jurkat, MCF7, C6, 3T3 and Hela cell lysates using CDK9 mouse mAb (1:2000 diluted).Predicted band size:43KDa.Observed band size:43, 50KDa.)

CDK9, Monoclonal Antibody (Cat# AAA476169)

Full Name
Anti-CDK9 Mouse mAb
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Sequence

Histone Deacetylase 1 (HDAC1), Active Protein (Cat# AAA2105171)

Full Name
Active Histone Deacetylase 1 (HDAC1)
Gene Names
HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1
Reactivity
Homo sapiens (Human)
Applications
Cell Culture, Activity Assays
Purity
>95%
Pricing
$220/0.01 mg | $470/0.05 mg | $725/0.1 mg | $895/0.2 mg | $1,745/0.5 mg | $2,600/1 mg | $6,360/5 mg
Immunohistochemistry (IHC) (MBS9600745 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

TAK1, Polyclonal Antibody (Cat# AAA9600745)

Full Name
Phospho-TAK1 (Ser439) Antibody
Gene Names
MAP3K7; CSCF; FMD2; TAK1; MEKK7; TGF1a
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF/ICC
Purity
Peptide affinity purification.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
SDS-Page (Recombinant Human DDR2 Kinase/CD167b Kinase/CD167b Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 65 kDa.)

DDR2 Kinase/CD167b Kinase/CD167b, Active Protein (Cat# AAA9139712)

Full Name
Recombinant Human DDR2 Kinase/CD167b Kinase/CD167b Protein
Gene Names
DDR2; TKT; WRCN; MIG20a; NTRKR3; TYRO10
Purity
>97% by SDS-PAGE.
Pricing
$185/0.02 mg | $245/0.05 mg | $325/0.1 mg | $470/0.2 mg | $855/0.5 mg | $3,690/5x0.5 mg
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