Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, using CDKL1 antibody.)
Rabbit anti-Human, Rat CDKL1 Polyclonal Antibody | anti-CDKL1 antibody
CDKL1 Antibody
Gene Names
CDKL1; P42; KKIALRE
Reactivity
Human, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CDKL1; Polyclonal Antibody; CDKL1 Antibody; CDC 2 related kinase 1; CDC 2 related kinase; CDC2 related kinase 1; CDC2 related kinase; CDK L1; CDKL 1; cdkl1; CDKL1_HUMAN; Cyclin dependent kinase like 1; cyclin-dependent kinase-like 1 (CDC2-related kinase); Cyclin-dependent kinase-like 1; EC 2.7.11.22; KKIALRE; p42; Protein kinase p42 KKIALRE; Serine/threonine protein kinase KKIALRE; Serine/threonine-protein kinase KKIALRE; anti-CDKL1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CDKL1 antibody detects endogenous levels of total CDKL1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
358
Applicable Applications for anti-CDKL1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Highly expressed in kidney, and to a lower extent in ovary.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Dog
Similarity
Pig (91%), Bovine (91%), Sheep (91%), Dog (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, using CDKL1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, using CDKL1 antibody.)
Immunofluorescene (IF)
(MBS9604695 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9604695 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-CDKL1 antibody
Function: ATP + a protein = ADP + a phosphoprotein.
Similarity: The [NKR]KIAxRE motif seems to be a cyclin-binding region. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Similarity: The [NKR]KIAxRE motif seems to be a cyclin-binding region. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 42 kD
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
cyclin-dependent kinase-like 1 isoform 1
NCBI Official Synonym Full Names
cyclin dependent kinase like 1
NCBI Official Symbol
CDKL1
NCBI Official Synonym Symbols
P42; KKIALRE
NCBI Protein Information
cyclin-dependent kinase-like 1
UniProt Protein Name
Cyclin-dependent kinase-like 1
Protein Family
UniProt Gene Name
CDKL1
NCBI Description
This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]
Research Articles on CDKL1
Similar Products
Product Notes
The CDKL1 cdkl1 (Catalog #AAA9604695) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDKL1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDKL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CDKL1 cdkl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDKL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
