Rabbit anti-Human Phospho-PKCalpha-S226 Antibody | anti-S226 antibody

Phospho-PKCalpha-S226 Polyclonal Antibody

Cat. Num. AAA9128997

• Gene Names: PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: Phospho-PKCalpha-S226; Phospho-PKCalpha-S226 Polyclonal Antibody ; AAG6; PKCA; PRKACA; PKC-alpha; anti-S226 antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 672

• Applicable Applications for anti-S226 antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Immunogen: A phospho specific peptide corresponding to residues surrounding S226 of human PKCalpha
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of 293 cells, using Phospho-PKCalpha-S226 antibody at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Related Product Information for anti-S226 antibody

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Product Categories/Family for anti-S226 antibody

Phosphorylated

NCBI and Uniprot Product Information

• NCBI GI #: 4506067
• NCBI GeneID: 5578
• NCBI Accession #: NP_002728.1
• NCBI GenBank Nucleotide #: NM_002737.2
• UniProt Accession #: P17252; Q15137; Q32M72; Q96RE4; B5BU22
• Molecular Weight: Calculated MW: 77kDa; Molecular Weight: 672
• NCBI Official Full Name: protein kinase C alpha type
• NCBI Official Synonym Full Names: protein kinase C alpha
• NCBI Official Symbol: PRKCA
• NCBI Official Synonym Symbols: AAG6; PKCA; PRKACA; PKC-alpha
• NCBI Protein Information: protein kinase C alpha type
• UniProt Protein Name: Protein kinase C alpha type
• UniProt Gene Name: PRKCA
• UniProt Synonym Gene Names: PKCA; PRKACA; PKC-A; PKC-alpha
• UniProt Entry Name: KPCA_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.

Protein type: Oncoprotein; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; AGC group; PKC family; Alpha subfamily

Chromosomal Location of Human Ortholog: 17q22-q23.2

Cellular Component: apical part of cell; cell soma; cytoplasm; cytosol; dendrite; endoplasmic reticulum; mitochondrial membrane; mitochondrion; nucleoplasm; perinuclear region of cytoplasm; photoreceptor outer segment; plasma membrane

Molecular Function: ATP binding; calcium-dependent protein kinase C activity; enzyme binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity; zinc ion binding

Biological Process: adenylate cyclase activation; angiogenesis; blood coagulation; cell adhesion; cellular calcium ion homeostasis; chondrocyte differentiation; energy reserve metabolic process; epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; fibroblast growth factor receptor signaling pathway; gene expression; inactivation of MAPK activity; induction of positive chemotaxis; innate immune response; mitotic cell cycle; mitotic nuclear envelope disassembly; negative regulation of adenylate cyclase activity; negative regulation of cell proliferation; negative regulation of glucose import; negative regulation of insulin receptor signaling pathway; nerve growth factor receptor signaling pathway; neutrophil chemotaxis; peptidyl-serine phosphorylation; phospholipase C activation; phototransduction, visible light; platelet activation; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of inflammatory response; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of macrophage differentiation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of insulin secretion; regulation of mRNA stability; regulation of muscle contraction; regulation of peptidyl-tyrosine phosphorylation; regulation of rhodopsin mediated signaling; regulation of the force of heart contraction; rhodopsin mediated signaling; signal transduction; synaptic transmission; vascular endothelial growth factor receptor signaling pathway