PGP9.5 active protein

PGP9.5, 1-223aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA203019

• Gene Names: UCHL1; NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PGP9.5; 1-223aa; Human; E Coli (Bioactivity Validated); ubiquitin carboxyl-terminal esterase L1; uCHL1; uCH-L1; EC 3.4.19.12; ubiquitin thioesterase L1; Neuron cytoplasmic protein 9.5; PGP 9.5; ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal esterase L1 Gracile axonal dystrophy; Park 5; Parkinson Disease 5; PGP95; uCH L1; Protein gene product 9.5; ubiquitin C terminal esterase L1; ubiquitin C terminal hydrolase (neuron specific); ubiquitin carboxyl terminal esterase L1; ubiquitin carboxyl terminal hydrolase isozyme L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1.; PGP9.5 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl pH8.0, 2mM EDTA
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA
• Sequence Length: 223

• Applicable Applications for PGP9.5 active protein: SDS-PAGE
• Antigen Species: Human
• Biological Activity: Specific activity is > 150 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PGP9.5 active protein

PGP9.5/uCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Recombinant PGP9.5 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PGP9.5 active protein

Neuroscience

References

Sakurai M., et al. (2006) Cell 119, 162-171.; Liu Y., et al. (2002) Cell. 111 (2):209-18.;

NCBI and Uniprot Product Information

• NCBI GI #: 21361091
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172
• NCBI GenBank Nucleotide #: NM_004181.4
• UniProt Accession #: P09936; Q4W5K6; Q71UM0
• Molecular Weight: 24.8 kDa (223 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L1
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5

NCBI Description

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Uniprot Description

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.