Western Blot (WB)
(Western blot analysis on Jurkat cell lysate using MAST3 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse MAST3 Polyclonal Antibody | anti-MAST3 antibody
MAST3 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MAST3; Polyclonal Antibody; MAST3 Antibody; EC 2.7.11.1; KIAA0561; MAST 3; MAST3_HUMAN; Microtubule associated serine/threonine protein kinase 3; Microtubule-associated serine/threonine-protein kinase 3; anti-MAST3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
MAST3 antibody detects endogenous levels of MAST3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1309
Applicable Applications for anti-MAST3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human MAST3
Predicted Cross Reactivity
Pig, Bovine, Sheep, Dog
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis on Jurkat cell lysate using MAST3 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis on Jurkat cell lysate using MAST3 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using MAST3 Antibody. Lane 1: B16F10 treated with blocking peptide. Lane 2: B16F10; Lane 3: 293; )
Western Blot (WB)
(Western blot analysis of extracts from various samples, using MAST3 Antibody. Lane 1: B16F10 treated with blocking peptide. Lane 2: B16F10; Lane 3: 293; )
Immunofluorescence (IF)
(MBS9600376 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9600376 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-MAST3 antibody
Description: MAST3 Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Interacts with PTEN. Note: This description may include information from UniProtKB.
Function: It is uncertain whether Met-1, Met-171 or Met-172 is the initiator.
Subunit Structure: Interacts with PTEN.
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Function: It is uncertain whether Met-1, Met-171 or Met-172 is the initiator.
Subunit Structure: Interacts with PTEN.
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 143 kDa
Predicted: 144 kDa
Predicted: 144 kDa
NCBI Official Full Name
microtubule-associated serine/threonine-protein kinase 3
NCBI Official Synonym Full Names
microtubule associated serine/threonine kinase 3
NCBI Official Symbol
MAST3
NCBI Protein Information
microtubule-associated serine/threonine-protein kinase 3
UniProt Protein Name
Microtubule-associated serine/threonine-protein kinase 3
Protein Family
UniProt Gene Name
MAST3
UniProt Synonym Gene Names
KIAA0561
Uniprot Description
CautionIt is uncertain whether Met-1, Met-171 or Met-172 is the initiator.
Research Articles on MAST3
Similar Products
Product Notes
The MAST3 mast3 (Catalog #AAA9600376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAST3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MAST3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MAST3 mast3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAST3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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