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Reactivity
Gene Name
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Host
Application
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918 results for "Mouse IgG2a Isotype Control" - showing 750-800
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-5 antibody 2E3. Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 2E3 (blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-5, Monoclonal Antibody (Cat# AAA488517)
Full Name
Anti-IL-5 [2E3], Mouse IgG2a, Kappa
Reactivity
Human
Applications
EIA
Purity
Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Overlay histogram showing human peripheral blood lymphocytes stained with 030846 (green line). The cells were incubated in 2%BSA to block non-specific protein-protein interactions followed by 030846 (1:50) for 60min at 37ºC. The secondary antibody used was Goat Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD3, Monoclonal Antibody (Cat# AAA6231246)
Full Name
CD3 (MaxLight 750)
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
FC/FACS
Purity
Purified by Protein G Affinity Chromatography
Pricing
$900/0.1 mL | $4,035/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with 138535 using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells. Immunohistochemical staining of formalin/paraffin tissues is enhanced by unmasking of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at room temp for 20min.)
Cyclin D1, Monoclonal Antibody (Cat# AAA6214981)
Full Name
Cyclin D1 (MaxLight 550)
Gene Names
CCND1; BCL1; PRAD1; U21B31; D11S287E
Reactivity
Human, Monkey, Mouse, Rat
Applications
FC/FACS, IF, IHC, WB
Purity
Purified by Protein A/G Affinity Chromatography.
Pricing
$785/0.1 mL | $3,520/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ErbB-4 expression in control (A) and ErbB-4 transfected (B) NIH/3T3 whole cell lysates.)
HER4/ErbB4, Monoclonal Antibody (Cat# AAA9435163)
Full Name
HER4/ErbB4 Antibody
Gene Names
ERBB4; HER4; p180erbB4
Reactivity
Human, Mouse, Rat
Applications
WB, IP, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in mouse spleen stained with D1876-49D.)
Dectin 2, Monoclonal Antibody (Cat# AAA6254857)
Full Name
Dectin 2 (Dendritic Cell-associated C-type Lectin 2, DC-associated C-type Lectin 2, Dectin-2, C-type Lectin Domain Family 6 Member A, CLEC6A, C-type Lectin Superfamily Member 10, CLECSF10) (MaxLight 550)
Gene Names
Clec4n; Nkcl; Clec6a; Clecsf10
Reactivity
Mouse
Applications
FC/FACS, IHC, IP
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
$865/0.1 mL | $3,885/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody chi220 (MBS488792) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of chi220 (MBS488792) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488792, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CD40, Monoclonal Antibody (Cat# AAA488791)
Full Name
Anti-CD40 [chi220]
Gene Names
CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Reactivity
Human
Applications
BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Jurkat cells with anti-CD18 antibody 60.3 (MBS488863) Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 60.3 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CD18, Monoclonal Antibody (Cat# AAA488863)
Full Name
Anti-CD18 [60.3]
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Reactivity
Human
Applications
EIA, WB, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of GAD65/GAD2 using anti-GAD65/GAD2 antibody (MBS1754020).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-GAD65/GAD2 antigen affinity purified monoclonal antibody (Catalog # MBS1754020) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for GAD65/GAD2 at approximately 62KD. The expected band size for GAD65/GAD2 is at 62KD. )
GAD65/GAD2, Monoclonal Antibody (Cat# AAA1754020)
Full Name
Anti-GAD65/GAD2 Antibody (monoclonal, 7G2)
Gene Names
GAD2; GAD65
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of IGF2R using anti-IGF2R antibody (MBS1753981).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human U87 whole cell lysatesLane 3: human HepG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- IGF2R antigen affinity purified monoclonal antibody (Catalog # MBS1753981) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for IGF2R at approximately 300KD. The expected band size for IGF2R is at 300KD. )
IGF2R, Monoclonal Antibody (Cat# AAA1753981)
Full Name
Anti-IGF2R Antibody (monoclonal, 4I11)
Gene Names
IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of INPPL1 using anti-INPPL1 antibody (MBS1753999).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Raji whole cell lysatesLane 3: human PC-3 whole cell lysatesLane 4: rat C6 whole cell lysatesLane 5: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- INPPL1 antigen affinity purified monoclonal antibody (Catalog # MBS1753999) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for INPPL1 at approximately 150KD. The expected band size for INPPL1 is at 150KD. )
INPPL1, Monoclonal Antibody (Cat# AAA1753999)
Full Name
Anti-INPPL1 Antibody (monoclonal, 8C13)
Gene Names
INPPL1; OPSMD; SHIP2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of MCM5 using anti-MCM5 antibody (MBS1754026).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysatesLane 2: rat thymus tissue lysatesLane 3: rat RH-35 whole cell lysatesLane 4: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- MCM5 antigen affinity purified monoclonal antibody (Catalog # MBS1754026) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for MCM5 at approximately 97KD. The expected band size for MCM5 is at 97KD. )
MCM5, Monoclonal Antibody (Cat# AAA1754026)
Full Name
Anti-MCM5 Antibody (monoclonal, 4G10)
Gene Names
MCM5; CDC46; P1-CDC46
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
IgG2a/IgG2b, Monoclonal Isotype Control (Cat# AAA8576104)
Full Name
Mouse IgG2a/IgG2b (FITC/PE)
Pricing
$310/0.1 mL | $1,330/5x0.1 mL
IgG2a/IgG1, Monoclonal Isotype Control (Cat# AAA8576106)
Full Name
Mouse IgG2a/IgG1 (FITC/PE)
Pricing
$310/0.1 mL | $1,330/5x0.1 mL
IgG2a kappa, Monoclonal Antibody (Cat# AAA567043)
Full Name
Mouse IgG2a kappa Isotype control, Recombinant monoclonal antibody (MOPC-21)
Applications
FC/FACS, WB
Purity
Protein A affinity purified
Pricing
$615/0.2 mg | $2,720/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-Podoplanin (MAP tag) antibody PMab-1 (MBS488581) Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (MBS488486) at 0.01ug/ml, 0.1ug/ml, 0.001ug/ml and 0.003ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Podoplanin, Monoclonal Antibody (Cat# AAA488582)
Full Name
Anti-Podoplanin (MAP tag) [PMab-1]
Gene Names
Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
Reactivity
Mouse
Applications
EIA, WB, IHC, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of APPL/APPL1 using anti-APPL/APPL1 antibody (MBS1754011).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysatesLane 2: human THP-1 whole cell lysatesLane 3: human PC-3 whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: rat brain tissue lysatesLane 6: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-APPL/APPL1 antigen affinity purified monoclonal antibody (Catalog # MBS1754011) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for APPL/APPL1 at approximately 85KD. The expected band size for APPL/APPL1 is at 85KD. )
APPL/APPL1, Monoclonal Antibody (Cat# AAA1754011)
Full Name
Anti-APPL/APPL1 Antibody (monoclonal, 5G11)
Gene Names
APPL1; APPL; DIP13alpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of U2AF65/U2AF2 using anti-U2AF65/U2AF2 antibody (MBS1754025).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human THP-1 whole cell lysatesLane 3: human U20S whole cell lysatesLane 4: human Jurkat whole cell lysatesLane 5: rat PC-12 whole cell lysatesLane 6: mouse brain tissue lysatesLane 7: rat brain tissue lysatesLane 8: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-U2AF65/U2AF2 antigen affinity purified monoclonal antibody (Catalog # MBS1754025) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for U2AF65/U2AF2 at approximately 65KD. The expected band size for U2AF65/U2AF2 is at 65KD. )
U2AF65/U2AF2, Monoclonal Antibody (Cat# AAA1754025)
Full Name
Anti-U2AF65/U2AF2 Antibody (monoclonal, 3G9)
Gene Names
U2AF2; U2AF65
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
IgG2a, Monoclonal Isotype Control (Cat# AAA8576108)
Full Name
Mouse IgG2a Isotype Control (FITC)
Pricing
$310/0.1 mL | $1,330/5x0.1 mL
IgG2a, Monoclonal Isotype Control (Cat# AAA8576099)
Full Name
Mouse IgG2a Isotype Control (PE/Cy5)
Pricing
$310/0.1 mL | $1,330/5x0.1 mL
IgG2b/IgG2a, Monoclonal Isotype Control (Cat# AAA8576105)
Full Name
Mouse IgG2b/IgG2a (FITC/PE)
Pricing
$310/0.1 mL | $1,330/5x0.1 mL
IgG2a/IgG2b, Monoclonal Isotype Control (Cat# AAA8576103)
Full Name
Mouse IgG2a/IgG2b (FITC/PE)
Pricing
$310/0.1 mL | $1,330/5x0.1 mL
Testing Data
IgG2a/IgG2b, Monoclonal Isotype Control (Cat# AAA4753786)
Full Name
Mouse IgG2a/IgG2b (FITC/PE)
Applications
FC/FACS
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
IgG2a, Monoclonal Isotype Control (Cat# AAA4753778)
Full Name
Mouse IgG2a Isotype Control (APC)
Applications
FC/FACS
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
IgG2a, Monoclonal Isotype Control (Cat# AAA4753782)
Full Name
Mouse IgG2a Isotype Control (PE/Cy5)
Applications
FC/FACS
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
IgG2a, Monoclonal Antibody (Cat# AAA9459473)
Full Name
APC mouse IgG2a Isotype Control
Reactivity
Human
Applications
FC/FACS, IF
Pricing
$290/100 Tests | $230/50 Tests | $1,160/5x100 Tests
Flow Cytometry (FC/FACS)
(C57BL/6 murine splenocytes are stained with PE/Cyanine7 Anti-Mouse CD38 Antibody[Used at 0.2ug/106 cells dilution] (filled gray histogram)or Rat IgG2a Isotype Control PE (empty black histogram).)
CD38, Monoclonal Antibody (Cat# AAA2563717)
Full Name
PE/Cyanine7 Anti-Mouse CD38 Antibody[NIMR5]
Gene Names
Cd38; ADPRC 1; Cd38-rs1
Reactivity
Mouse
Applications
FC/FACS/FCM
Pricing
$165/0.05 mg | $230/0.1 mg | $340/0.2 mg | $130/0.025 mg | $1,510/5x0.2 mg
Flow Cytometry (FC/FACS)
(Example of nonspecific mouse IgG2a (MOPC-173) PerCP signal on human peripheral blood; surface staining, 9 ug/ml.)
IgG2a, Monoclonal Isotype Control (Cat# AAA1801357)
Full Name
Mouse IgG2a Isotype Control PerCP
Applications
FC/FACS
Purity
The purified antibody is conjugated with PerCP under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Pricing
$290/0.1 mg | $1,095/5x0.1 mg
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
CD31, Monoclonal Antibody (Cat# AAA225961)
Full Name
RAT ANTI MOUSE CD31: Biotin
Reactivity
Mouse
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
CD31, Monoclonal Antibody (Cat# AAA225965)
Full Name
RAT ANTI MOUSE CD31
Reactivity
Mouse
Applications
IHC, FC/FACS, IP
Pricing
$195/0.025 mg | $730/5x0.025 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Daudi cells with anti-CD23 antibody CAT-13.9C1 (MBS488719) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version CAT-13.9C1 (MBS488719) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488719, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
CD23, Monoclonal Antibody (Cat# AAA488718)
Full Name
Anti-CD23 [CAT-13.9C1]
Gene Names
FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
Reactivity
Human
Applications
IF
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PROM1/CD133 using anti-PROM1/CD133 antibody (MBS1753997).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human CACO-2 whole cell lysatesLane 2: human SW620 whole cell lysatesLane 3: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- PROM1/CD133 antigen affinity purified monoclonal antibody (Catalog # MBS1753997) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for PROM1/CD133 at approximately 120KD. The expected band size for PROM1/CD133 is at 120KD. )
PROM1/CD133, Monoclonal Antibody (Cat# AAA1753997)
Full Name
Anti-PROM1/CD133 Antibody (monoclonal, 8B6)
Gene Names
PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
Reactivity
Human, Mouse
Applications
WB, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of RPL13A using anti-RPL13A antibody (MBS1754023).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hek293 whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human K562 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- RPL13A antigen affinity purified monoclonal antibody (Catalog # MBS1754023) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for RPL13A at approximately 24KD. The expected band size for RPL13A is at 24KD. )
RPL13A, Monoclonal Antibody (Cat# AAA1754023)
Full Name
Anti-RPL13A Antibody (monoclonal, 4C9)
Gene Names
RPL13A; L13A; TSTA1
Reactivity
Human
Applications
WB, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (MBS1754035).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human Raji whole cell lysatesLane 5: human HEPG2 whole cell lysatesLane 6: rat PC-12 whole cell lysatesLane 7: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-eRF1/ETF1 antigen affinity purified monoclonal antibody (Catalog # MBS1754035) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for eRF1/ETF1 at approximately 49KD. The expected band size for eRF1/ETF1 is at 49KD. )
eRF1/ETF1, Monoclonal Antibody (Cat# AAA1754035)
Full Name
Anti-eRF1/ETF1 Antibody (monoclonal, 3B6)
Gene Names
ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of human peripheral blood monocytes using anti-CD18 antibody 1B4 Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of 1B4 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD18, Monoclonal Antibody (Cat# AAA488525)
Full Name
Anti-CD18 [1B4], Rabbit IgG, Kappa
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Reactivity
Human, Rhesus Monkey
Applications
IP, EIA, FC/FACS, IF
Purity
Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
IgG2a, Monoclonal Isotype Control (Cat# AAA8576095)
Full Name
Mouse IgG2a Isotype Control (APC)
Pricing
$310/0.1 mL | $1,330/5x0.1 mL
Testing Data
IgG2b/IgG2a, Monoclonal Isotype Control (Cat# AAA4753787)
Full Name
Mouse IgG2b/IgG2a (FITC/PE)
Applications
FC/FACS
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
IgG2a, Monoclonal Isotype Control (Cat# AAA4753794)
Full Name
Mouse IgG2a Isotype Control (PE)
Applications
FC/FACS
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Flow Cytometry (FC/FACS)
(Human peripheral blood lymphocytes were stained with PE OKT3 with relevant isotype control in Gray.)
CD3, Monoclonal Antibody (Cat# AAA696589)
Full Name
Anti-Human CD3 PE
Reactivity
Human
Applications
FC/FACS
Purity
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Pricing
$210/100 Tests | $145/25 Tests | $935/5x100 Tests
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
CD31, Monoclonal Antibody (Cat# AAA225964)
Full Name
RAT ANTI MOUSE CD31: RPE
Reactivity
Mouse
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
CD31, Monoclonal Antibody (Cat# AAA225960)
Full Name
RAT ANTI MOUSE CD31
Reactivity
Mouse
Applications
IHC, FC/FACS, IP
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-IL2R beta antibody 5H4 (MBS488672) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 5H4 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
IL2R beta, Monoclonal Antibody (Cat# AAA488672)
Full Name
Anti-IL2R beta [5H4]
Gene Names
Il2rb; p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of RAW 264.7 cells using anti-Transferrin receptor antibody (MBS488646) R17 217.1.3. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of R17 217.1.3 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Transferrin receptor, Monoclonal Antibody (Cat# AAA488646)
Full Name
Anti-Transferrin receptor [R17 217.1.3]
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of IRF3 using anti-IRF3 antibody (MBS1753961).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: rat C6 whole cell lysatesLane 3: mouse lung tissue lysatesLane 4: mouse brain tissue lysatesLane 5: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- IRF3 antigen affinity purified monoclonal antibody (Catalog # MBS1753961) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for IRF3 at approximately 50-55KD. The expected band size for IRF3 is at 50-55KD. )
IRF3, Monoclonal Antibody (Cat# AAA1753961)
Full Name
Anti-IRF3 Antibody (monoclonal, 3B4)
Gene Names
Irf3; IRF-3; C920001K05Rik
Reactivity
Mouse, Rat
Applications
WB, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of RALBP1 using anti-RALBP1 antibody (MBS1753992).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysatesLane 2: human U20S whole cell lysatesLane 3: human Daudi whole cell lysatesLane 4: human A431 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- RALBP1 antigen affinity purified monoclonal antibody (Catalog # MBS1753992) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for RALBP1 at approximately 95KD. The expected band size for RALBP1 is at 95KD. )
RALBP1, Monoclonal Antibody (Cat# AAA1753992)
Full Name
Anti-RALBP1 Antibody (monoclonal, 2E11)
Gene Names
RALBP1; RIP1; RLIP1; RLIP76
Reactivity
Human
Applications
WB, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of UBE3A using anti-UBE3A antibody (MBS1753974).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human Raji whole cell lysatesLane 4: monkey COS-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- UBE3A antigen affinity purified monoclonal antibody (Catalog # MBS1753974) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for UBE3A at approximately 100KD. The expected band size for UBE3A is at 100KD. )
UBE3A, Monoclonal Antibody (Cat# AAA1753974)
Full Name
Anti-UBE3A Antibody (monoclonal, 8I3)
Gene Names
UBE3A; AS; ANCR; E6-AP; HPVE6A; EPVE6AP; FLJ26981; UBE3A
Reactivity
Human, Monkey
Applications
WB, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of MPI using anti-MPI antibody (MBS1753965).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human CACO-2 whole cell lysatesLane 3: human HEK293 whole cell lysatesLane 4: human U87 whole cell lysatesLane 5: rat brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- MPI antigen affinity purified monoclonal antibody (Catalog # MBS1753965) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for MPI at approximately 45KD. The expected band size for MPI is at 45KD. )
MPI, Monoclonal Antibody (Cat# AAA1753965)
Full Name
Anti-MPI Antibody (monoclonal, 5G5)
Gene Names
MPI; PMI; PMI1; CDG1B
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (MBS1754001).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: monkey COS-7 whole cell lysatesLane 4: rat PC-12 whole cell lysatesLane 5: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- PP2A-alpha/PPP2CA antigen affinity purified monoclonal antibody (Catalog # MBS1754001) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for PP2A-alpha/PPP2CA at approximately 36KD. The expected band size for PP2A-alpha/PPP2CA is at 36KD. )
PP2A-alpha/PPP2CA, Monoclonal Antibody (Cat# AAA1754001)
Full Name
Anti-PP2A-alpha/PPP2CA Antibody (monoclonal, 3B6)
Gene Names
PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
IgG2a, Monoclonal Isotype Control (Cat# AAA697144)
Full Name
Mouse IgG2a Isotype Control PE
Reactivity
Mouse
Applications
FC/FACS
Purity
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Pricing
$215/0.1 mg | $955/5x0.1 mg
Testing Data
IgG2a/IgG1, Monoclonal Isotype Control (Cat# AAA4753788)
Full Name
Mouse IgG2a/IgG1 (FITC/PE)
Applications
FC/FACS
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Flow Cytometry (FC/FACS)
(Human red blood cells were stained with anti- CD235a (clone: YTH 89.1)(filled histogram) or Rat IgG2b isotype control (open histogram).)
TCP-1B, Monoclonal Antibody (Cat# AAA524526)
Full Name
Anti-TCP-1B, (clone: F39 P7 F11)(mouse IgG2a)
Reactivity
Human
Applications
WB
Purity
Purified
Pricing
$380/0.1 mg | $1,485/5x0.1 mg
