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2525 results for "protein-decapentaplegic" - showing 700-750
Smad2, ELISA Kit (Cat# AAA9500992)
Full Name
Phospho-Smad2 (Ser467) Colorimetric Cell-Based ELISA Kit
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Pricing
$575/2x96 Wells | $2,580/10x96 Wells | $5,065/20x96 Wells
Mothers Against Decapentaplegic Homolog 3/SMAD3, Recombinant Protein (Cat# AAA2552970)
Full Name
Recombinant Human Mothers Against Decapentaplegic Homolog 3/SMAD3 Protein (N-6His-DYKDDDDK)
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
$155/0.01 mg | $295/0.05 mg | $1,320/5x0.05 mg
Typical Testing Data/Standard Curve (for reference only)
Mothers Against Decapentaplegic Homolog 1 (Smad1), RTU ELISA Kit (Cat# AAA4502548)
Full Name
Rat Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit
Gene Names
Smad1; Madh1
Reactivity
Rat
Pricing
$585/96 Tests | $440/48 Tests | $5,160/10x96 Tests | $2,645/5x96 Tests
Western Blot (WB)
(Western blot analysis of Smad1 phosphorylation expression in Mouse muscletissue lysates, The lane on the left is treated with the antigen-specific peptide.)
Smad1, Antibody (Cat# AAA8511633)
Full Name
Phospho-Smad1 (Ser187) Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Immunogen affinity purified
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of SARA expression in Hela (A) whole cell lysates.)
SARA, Polyclonal Antibody (Cat# AAA8292687)
Full Name
Anti-SARA Antibody
Gene Names
ZFYVE9; NSP; SARA; MADHIP; SMADIP; PPP1R173
Reactivity
Human, Mouse, Rat
Applications
WB, IP
Purity
Purified by immunogen affinity chromatography
Pricing
$200/0.05 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Smad2, Polyclonal Antibody (Cat# AAA8525310)
Full Name
Smad2 Polyclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of 1) A431, 2) Hela, 3)C2C12, 4)3T3, 5) PC12, 6) Rat Brain Tissue, with Smad3 Rabbit pAb E12-393 diluted at 1: 2, 000.)
Smad3, Polyclonal Antibody (Cat# AAA8510442)
Full Name
Smad3 Rabbit Polyclonal Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Rat, Mouse
Applications
WB
Purity
Affinity Purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(The TFact Smad1 DNA-Binding ELISA detects active Smad1 in HepG2 and Jurkat Nuclear Extract. The HepG2 and Jurkat cells were grown 3 days in DMEM and RPMI 1640 with 10% FBS and harvested for nuclear extract.)
Smad1, ELISA Kit (Cat# AAA9502244)
Full Name
Smad1 TFact DNA-Binding ELISA Kit
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Pricing
$590/96-Strip-Wells | $2,650/5x96-Strip-Wells | $5,205/10x96-Strip-Wells
Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: Smad1 knockout 293T cell lysate; Predicted MW: 52kd Observed MW: 60kd Primary Ab: 1ug/ml Rabbit Anti-Bovine Smad1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Mothers Against Decapentaplegic Homolog 1 (Smad1), Polyclonal Antibody (Cat# AAA2045473)
Full Name
PE-Linked Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Cattle
Applications
WB, ICC, IHC, ELISA
Pricing
$275/0.1 mL | $370/0.2 mL | $695/0.5 mL | $860/1 mL | $2,395/5x1 mL
Western Blot (WB)
(Western blot analysis of lysates from HeLa cells, using Smad1/5/9 Antibody. The lane on the right is blocked with the synthesized peptide.)
Smad1/5/9, Polyclonal Antibody (Cat# AAA9505262)
Full Name
Smad1/5/9 Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB)
(SMAD3 monoclonal antibody (M05), clone 4D5 Western Blot analysis of SMAD3 expression in PC-12.)
SMAD3, Monoclonal Antibody (Cat# AAA6163797)
Full Name
SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (AP)
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Applications
WB
Purity
Purified
Pricing
$795/0.1 mL | $3,570/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad1 (Phospho-Ser187) Antibody. The picture on the right is blocked with the phospho peptide.)
Smad1, Polyclonal Antibody (Cat# AAA9502793)
Full Name
Smad1 (Phospho-Ser187) Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Smad2, ELISA Kit (Cat# AAA9501316)
Full Name
Phospho-Smad2 (Ser250) Colorimetric Cell-Based ELISA Kit
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Pricing
$575/2x96 Wells | $2,580/10x96 Wells | $5,065/20x96 Wells
Western Blot (WB)
(Western blot analysis of extracts of HeLa cellline, using SMAD2 antibody.)
SMAD2, Polyclonal Antibody (Cat# AAA8527330)
Full Name
SMAD2 Polyclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis Smad1 (Phospho-Ser214) using 293 whole cell lysates)
Smad1, Polyclonal Antibody (Cat# AAA9416524)
Full Name
Smad1 (Phospho-Ser214) Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis Smad2 (Phospho-Ser245) using Serum treated LOVO whole cell lysates)
Smad2, Polyclonal Antibody (Cat# AAA9416525)
Full Name
Smad2 (Phospho-Ser245) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad2 (Phospho-Ser467) Antibody. The picture on the right is blocked with the phospho peptide.)
Smad2, Polyclonal Antibody (Cat# AAA9502348)
Full Name
Smad2 (Phospho-Ser467) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Smad1/5/9, Blocking Peptide (Cat# AAA9619276)
Full Name
Phospho-Smad1/5/9 (Ser463+Ser465) Blocking Peptide
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
Blocking/Control
Purity
The purity is >90%,tested by HPLC and MS.
Pricing
$380/1 mg | $1,650/5x1 mg
Western Blot (WB)
(Western Blot analysis in HepG2 and mouse C2C12 cell line lysates using MBS6348740 (35ug/lane) using MBS6348740. This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).)
SMAD3, Polyclonal Antibody (Cat# AAA6348740)
Full Name
SMAD3 (S208) (SMAD3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3) (FITC)
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human
Applications
WB, IF, IHC
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
Smad2, Polyclonal Antibody (Cat# AAA8520135)
Full Name
Smad2 (phospho Ser250) Polyclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Mothers against decapentaplegic homolog 1, Protein (Cat# AAA2888764)
Full Name
Mothers against decapentaplegic homolog 1
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Smad1, ELISA Kit (Cat# AAA9500549)
Full Name
Smad1 Colorimetric Cell-Based ELISA Kit
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Pricing
$470/1x96 Wells | $2,110/5x96 Wells | $4,150/10x96 Wells
Western Blot (WB)
Smad2, Polyclonal Antibody (Cat# AAA8510456)
Full Name
Smad2 Rabbit Polyclonal Antibody
Reactivity
Human, Rat, Mouse
Applications
WB, IHC
Pricing
$330/0.1 mg | $1,425/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, using Smad2 Antibody. The lane on the right is blocked with the synthesized peptide.)
Smad2, Polyclonal Antibody (Cat# AAA9503907)
Full Name
Smad2 Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
SMAD1, Recombinant Protein (Cat# AAA8249807)
Full Name
Recombinant Human SMAD1 Protein
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human
Applications
EIA, WB, MS
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Pricing
$285/0.01 mg | $680/0.05 mg | $2,500/0.5 mg | $11,210/5x0.5 mg
Smad1, Polyclonal Antibody (Cat# AAA8525305)
Full Name
Smad1 Polyclonal Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Smad1, Polyclonal Antibody (Cat# AAA8526328)
Full Name
Smad1 Polyclonal Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of Smad2/3 expression in UV treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Smad2/3, Antibody (Cat# AAA8512389)
Full Name
Smad2/3 Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Immunogen affinity purified
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
SMAD4, Recombinant Protein (Cat# AAA8249808)
Full Name
Recombinant Human SMAD4 Protein
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
EIA, WB, MS
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Pricing
$220/0.01 mg | $470/0.05 mg | $2,440/0.5 mg | $10,935/5x0.5 mg
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using Smad1 Antibody. The picture on the right is blocked with the synthesized peptide.)
Smad1, Polyclonal Antibody (Cat# AAA9503904)
Full Name
Smad1 Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse
Applications
WB, IF, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from RAW264.7 cells, using Smad2/3 (Phospho-Thr8) antibody.)
Smad2/3, Antibody (Cat# AAA8509690)
Full Name
Smad2/3 (Phospho-Thr8) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000583)
Full Name
SMAD4 Antibody: FITC
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Smad1 expression in 293T cell lysate (MBS179427).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMAD1 )
Smad1, Monoclonal Antibody (Cat# AAA179427)
Full Name
Anti-Smad1 Rabbit Monoclonal Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
SMAD4 / Mothers Against Decapentaplegic Homolog 4, Recombinant Protein (Cat# AAA2903071)
Full Name
Human SMAD4 / Mothers Against Decapentaplegic Homolog 4 Recombinant Protein
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
SMAD1, Monoclonal Antibody (Cat# AAA8528512)
Full Name
Mouse Monoclonal Antibody to SMAD1
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
WB
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
SMAD7, Polyclonal Antibody (Cat# AAA8536701)
Full Name
SMAD7
Gene Names
SMAD7; CRCS3; MADH7; MADH8
Reactivity
Mouse, Rat
Applications
WB
Purity
Affinity purification
Pricing
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
SMAD7, Polyclonal Antibody (Cat# AAA8535401)
Full Name
SMAD7
Gene Names
SMAD7; CRCS3; MADH7; MADH8
Reactivity
Human
Applications
WB
Purity
Affinity purification
Pricing
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Smad1/5/9, ELISA Kit (Cat# AAA9500866)
Full Name
Smad1/5/9 Colorimetric Cell-Based ELISA Kit
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Pricing
$470/1x96 Wells | $2,110/5x96 Wells | $4,150/10x96 Wells
Western Blot (WB)
(Western blot analysis of Smad1 phosphorylation expression in Serum treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Smad1, Antibody (Cat# AAA8511632)
Full Name
Phospho-Smad1 (Ser465) Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat stomach, using Smad2 Antibody(MBS179094)SMAD2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD2 Antibody (MBS179094)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Smad2, Monoclonal Antibody (Cat# AAA179094)
Full Name
Anti-Smad2 Rabbit Monoclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various celllines, using SMAD4 antibody.)
SMAD4, Antibody (Cat# AAA8516876)
Full Name
SMAD4 Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Smad4, Monoclonal Antibody (Cat# AAA8533709)
Full Name
Smad4
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$350/0.1 mL | $570/0.1 mL (Biotin) | $1,515/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Testing Data
SMAD3, Recombinant Protein (Cat# AAA515663)
Full Name
SMAD3 (del SXS) Protein
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Applications
WB
Pricing
$295/0.02 mg | $485/0.05 mg | $1,955/5x0.05 mg
Western Blot (WB)
(Western blot analysis of Smad1 expression in EGF treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Smad1, Antibody (Cat# AAA8512456)
Full Name
Smad1 Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(The TFact Smad1 (Phospho-Ser187) DNA-Binding ELISA Kits detect active Smad1 in HepG2 Nuclear Extract. The HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. )
Smad1, ELISA Kit (Cat# AAA9502243)
Full Name
Smad1 (Phospho-Ser187) TFact DNA-Binding ELISA Kit
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Pricing
$620/96-Strip-Wells | $2,790/5x96-Strip-Wells | $5,500/10x96-Strip-Wells
Western Blot (WB)
(Western blot analysis of lysates from HT-29 cells, using Smad3 Antibody. The lane on the right is blocked with the synthesized peptide.)
Smad3, Polyclonal Antibody (Cat# AAA9503912)
Full Name
Smad3 Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB)
(Western blot analysis of SMAD9 expression in HepG2 (A), A549 (B) whole cell lysates.)
SMAD9, Polyclonal Antibody (Cat# AAA8292477)
Full Name
Anti-SMAD9 Antibody
Gene Names
SMAD9; PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B; SMAD8/9
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC
Purity
Purified by immunogen affinity chromatography
Pricing
$200/0.05 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Smad2, Monoclonal Antibody (Cat# AAA8533713)
Full Name
Smad2
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Rat
Applications
WB, ICC, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
$350/0.1 mL | $570/0.1 mL (Biotin) | $1,515/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Figure 1. Western blot analysis of SMAD1 using anti-SMAD1 antibody (MBS1750468).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: mouse brain tissue lysate,Lane 4: mouse kidney tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: human Hela cell lysate,Lane 7: human A375 cell lysate,Lane 8: human HepG2 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMAD1 at approximately 52,60KD. The expected band size for SMAD1 is at 48, 52, 60KD. )
SMAD1/SMAD5, Polyclonal Antibody (Cat# AAA1750468)
Full Name
Anti-SMAD1/SMAD5 Picoband antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Smad2, Polyclonal Antibody (Cat# AAA2547879)
Full Name
Smad2 Polyclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Applications
WB, IHC
Pricing
$405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
