Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: Smad1 knockout 293T cell lysate; Predicted MW: 52kd Observed MW: 60kd Primary Ab: 1ug/ml Rabbit Anti-Bovine Smad1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Rabbit anti-Cattle Mothers Against Decapentaplegic Homolog 1 (Smad1) Polyclonal Antibody | anti-Smad1 antibody
PE-Linked Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: Smad1 knockout 293T cell lysate; Predicted MW: 52kd Observed MW: 60kd Primary Ab: 1ug/ml Rabbit Anti-Bovine Smad1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: Smad1 knockout 293T cell lysate; Predicted MW: 52kd Observed MW: 60kd Primary Ab: 1ug/ml Rabbit Anti-Bovine Smad1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
SMAD1: transcription factor phosphorylated and activated by bone morphogenetic protein type 1 receptor kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated forms dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 4q31.21
Cellular Component: cytoplasm; cytosol; nuclear inner membrane; nucleoplasm; nucleus; protein complex
Molecular Function: identical protein binding; protein binding; protein kinase binding; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
Biological Process: BMP signaling pathway; embryonic pattern specification; positive regulation of transcription from RNA polymerase II promoter; primary microRNA processing; SMAD protein complex assembly
Research Articles on Smad1
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Product Notes
The Smad1 smad1 (Catalog #AAA2045473) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PE-Linked Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1) reacts with Cattle and may cross-react with other species as described in the data sheet. AAA Biotech's Mothers Against Decapentaplegic Homolog 1 (Smad1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (ELISA). Researchers should empirically determine the suitability of the Smad1 smad1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mothers Against Decapentaplegic Homolog 1 (Smad1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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