Rabbit anti-Human SMAD3 Polyclonal Antibody | anti-SMAD3 antibody

SMAD3 (S208) (SMAD3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3) (FITC)

Cat. Num. AAA6348740

• Gene Names: SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SMAD3; Polyclonal Antibody; SMAD3 (S208) (SMAD3; MADH3; Mothers against decapentaplegic homolog 3; JV15-2; SMAD family member 3) (FITC); SMAD family member 3; anti-SMAD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SMAD3. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-SMAD3 antibody: FLISA, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Other potential application, though not tested is Direct Flow Cytometry. Other applications not tested.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 186-215 of human SMAD3, UniProt Accession #P84022. Species sequence homology: chicken, porcine and rat
• Conjugate: FITC
• Grade: Affinity Purified
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4°C if to be used immediately within twoweeks. For long-term storage, aliquot to avoid repeatedfreezing and thawing and store at -20°C. Aliquots are stable at-20°C for 12 months after receipt. Dilute required amount onlyprior to immediate use. Further dilutions can be made in assaybuffer. Caution: FITC conjugates are sensitive to light. Formaximum recovery of product, centrifuge the original vial afterthawing and prior to removing the cap.Note: Applications are based on unconjugated antibody.

Western Blot (WB)

(Western Blot analysis in HepG2 and mouse C2C12 cell line lysates using MBS6348740 (35ug/lane) using MBS6348740. This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of MBS6348740 in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MBS6348740 with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MBS6348740 using MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SMAD3 antibody

SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Product Categories/Family for anti-SMAD3 antibody

Antibodies; Smad Proteins

References

1. Zhang, M., et al. J. Biol. Chem. 285(12):8703-8710(2010). 2. Daly, A.C., et al. J. Biol. Chem. 285(9):6489-6497(2010). 3. Heikkinen, P.T., et al. J. Biol. Chem. 285(6):3740-3749(2010). 4. Tseng, Z.H., et al. Heart Rhythm 6(12):1745-1750(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 223029440
• NCBI GeneID: 4088
• NCBI Accession #: NP_001138574.1
• NCBI GenBank Nucleotide #: NM_001145102.1
• UniProt Accession #: P84022; O09064; O09144; O14510; O35273; Q92940; Q93002; A8K4B6; B7Z4Z5; B7Z6M9; B7Z9Q2; F5H383
• NCBI Official Full Name: mothers against decapentaplegic homolog 3 isoform 2
• NCBI Official Synonym Full Names: SMAD family member 3
• NCBI Official Symbol: SMAD3
• NCBI Official Synonym Symbols: LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
• NCBI Protein Information: mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentapleg
• UniProt Protein Name: Mothers against decapentaplegic homolog 3
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD3
• UniProt Synonym Gene Names: MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
• UniProt Entry Name: SMAD3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Uniprot Description

SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Protein type: DNA-binding; Transcription factor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 15q22.33

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; plasma membrane; nuclear inner membrane; cytosol; nucleus; receptor complex

Molecular Function: identical protein binding; ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; beta-catenin binding; protein kinase binding; transcription factor binding; phosphatase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; collagen binding; protein binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; bHLH transcription factor binding; transforming growth factor beta receptor binding; transcription factor activity

Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; embryonic foregut morphogenesis; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; regulation of transcription from RNA polymerase II promoter; positive regulation of chondrocyte differentiation; mesoderm formation; negative regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein catabolic process; endoderm development; negative regulation of apoptosis; evasion of host defenses by virus; wound healing; T cell activation; negative regulation of transcription from RNA polymerase II promoter; primary microRNA processing; regulation of epithelial cell proliferation; positive regulation of focal adhesion formation; negative regulation of protein amino acid phosphorylation; ureteric bud development; transport; positive regulation of transforming growth factor-beta3 production; thyroid gland development; positive regulation of transcription factor import into nucleus; heart looping; caspase activation; transcription initiation from RNA polymerase II promoter; intercellular junction assembly and maintenance; regulation of transforming growth factor-beta2 production; regulation of immune response; regulation of binding; protein stabilization; in utero embryonic development; positive regulation of bone mineralization; liver development; SMAD protein complex assembly; immune system development; positive regulation of interleukin-1 beta production; negative regulation of inflammatory response; response to hypoxia; gene expression; immune response; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration

Disease: Loeys-dietz Syndrome 3