Western Blot (WB)
Smad2 Polyclonal Antibody | anti-Smad2 antibody
Smad2 Rabbit Polyclonal Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry
Synonyms
Smad2; Polyclonal Antibody; Smad2 Rabbit Polyclonal Antibody; hMAD2 antibody; JV18 antibody; MAD antibody; MADH2 antibody; MGC22139 antibody; anti-Smad2 antibody
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Antibody can detects endogenous Smad2 protein.
Form/Format
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Sequence Length
467
Applicable Applications for anti-Smad2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1,000-2,000
IHC: 1:100-200
IHC: 1:100-200
Preparation and Storage
Store at-20 degree C for 1 year. Do not aliquot the antibody.
Western Blot (WB)
Western Blot (WB)
Immunohistochemisry (IHC)
(Western blot analysis of 1) Hela, 2)Jurkat, 3) C2C12, 4) PC12 with Smad2 Rabbit pAb E12-434 diluted at 1: 2, 000.Immunohistochemical analysis of paraffin-embedded Mouse Heart Tissue using Smad2 (E12-434)Rabbit pAb diluted at 1: 200.)
Immunohistochemisry (IHC)
(Western blot analysis of 1) Hela, 2)Jurkat, 3) C2C12, 4) PC12 with Smad2 Rabbit pAb E12-434 diluted at 1: 2, 000.Immunohistochemical analysis of paraffin-embedded Mouse Heart Tissue using Smad2 (E12-434)Rabbit pAb diluted at 1: 200.)
Related Product Information for anti-Smad2 antibody
SMAD2 is a protein that in humans is encoded by the SMAD2 gene. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
60KD
NCBI Official Full Name
Smad2
UniProt Protein Name
Mothers against decapentaplegic homolog 2
Protein Family
UniProt Gene Name
SMAD2
UniProt Synonym Gene Names
MADH2; MADR2; MAD homolog 2; Mothers against DPP homolog 2; hMAD-2; SMAD 2; Smad2; hSMAD2
Uniprot Description
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Similar Products
Product Notes
The Smad2 smad2 (Catalog #AAA8510456) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Smad2 Rabbit Polyclonal Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Smad2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1,000-2,000 IHC: 1:100-200. Researchers should empirically determine the suitability of the Smad2 smad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
