Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
790 results for "Methylated Antibodies" - showing 650-700
Western Blot (WB)
(Western blot analysis of MGMT antibody (C-term) in CEM cell line lysates (35ug/lane). MGMT (arrow) was detected using the purified Pab.)
MGMT, Polyclonal Antibody (Cat# AAA6488055)
Full Name
MGMT, CT (O-6-Methylguanine DNA Methyltransferase, 6-O-Methylguanine DNA Methyltransferase, Methylated DNA Protein Cysteine Methyltransferase, Methylguanine DNA Methyltransferase, O-6-Methylguanine DNA Alkyltransferase) (Biotin)
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Purified by Ammonium Sulfate Precipitation.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
Dot Blot (DB)
(Dot blot analysis using MBS646539 on nitrocellulose membrane. 50ng of methylated peptide or non-methylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug/ml.)
H4, Polyclonal Antibody (Cat# AAA6305290)
Full Name
H4 (K20 [Me2]) (HIST1H4A, H4/A, H4FA, Histone H4) (MaxLight 490)
Gene Names
HIST2H4B; H4/o
Reactivity
Human
Applications
IHC, FC/FACS, WB, DB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
pan Symmetric-DiMethyl-Arginine, Polyclonal Antibody (Cat# AAA8578940)
Full Name
pan Symmetric-DiMethyl-Arginine pAb
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
WB
Purity
Affinity purification
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Histone H3.1, Polyclonal Antibody (Cat# AAA6420748)
Full Name
Histone H3.1 (HIST1H3J, Histone H3R2me1, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l, HIST3H3) (MaxLight 405)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Histone H4, Polyclonal Antibody (Cat# AAA6480796)
Full Name
Histone H4, trimethyl (Lys20) (MaxLight 650)
Reactivity
Human, Mouse, Rat
Applications
WB, DB, ChIP
Purity
Purified by affinity chromatography.
Pricing
$1,040/0.1 mL | $4,675/5x0.1 mL
Histone H4R3me2s, Polyclonal Antibody (Cat# AAA6420859)
Full Name
Histone H4R3me2s (H4, H4/n, H4F2, H4FN, FO108, HIST2H4) (MaxLight 490)
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC
Purity
Purified by affinity chromatography.
Pricing
$955/0.1 mL | $4,295/5x0.1 mL
Histone H3.1, Polyclonal Antibody (Cat# AAA6420642)
Full Name
Histone H3.1 (HIST1H3J, Histone H3K4me3, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l, HIST3H3) (MaxLight 750)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC, IP, ChIP
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
GLUT1, Polyclonal Antibody (Cat# AAA9459389)
Full Name
GLUT1(Mono-methyl-K245) Antibody
Gene Names
SLC2A1; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT1DS
Reactivity
Human
Applications
WB
Purity
The antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$355/0.1 mL | $1,440/5x0.1 mL
Histone H3.1, Polyclonal Antibody (Cat# AAA6420674)
Full Name
Histone H3.1 (HIST1H3J, Histone H3K9me1, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l, HIST3H3) (MaxLight 650)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC, IP, ChIP
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Histone H3.1, Polyclonal Antibody (Cat# AAA6420773)
Full Name
Histone H3.1 (HIST1H3J, Histone H3R8me2a, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l, HIST3H3) (MaxLight 650)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Histone H3.1, Polyclonal Antibody (Cat# AAA6420576)
Full Name
Histone H3.1 (HIST1H3J, Histone H3K27me3, H3/j, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l, HIST3H3) (MaxLight 750)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC, IP, ChIP
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CELF1 antibody.)
CELF1, Monoclonal Antibody (Cat# AAA6188060)
Full Name
CELF1 (CUGBP, Elav-like Family Member 1, CUGBP, NAB50, NAPOR, CUG-BP, CUGBP1, hNab50, BRUNOL2, EDEN-BP) (FITC)
Gene Names
CELF1; CUGBP; NAB50; NAPOR; CUG-BP; CUGBP1; hNab50; BRUNOL2; EDEN-BP
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Purified by Affinity Chromatography
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4R3me2s antibody.)
Histone H4R3me2s, Polyclonal Antibody (Cat# AAA6420857)
Full Name
Histone H4R3me2s (H4, H4/n, H4F2, H4FN, FO108, HIST2H4) (HRP)
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC
Purity
Purified by affinity chromatography.
Pricing
$955/0.1 mL | $4,295/5x0.1 mL
Western Blot (WB)
(WB: recombinant Histone H3 alone (Lane 1) and recombinant Histone H3 in the presence of CARM1 (Lane 2) were probed MBS614993 Histone H3 (Arg17).)
Histone H3, Polyclonal Antibody (Cat# AAA6480742)
Full Name
Histone H3, Dimethyl (Arg17) (MaxLight 750)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human
Applications
ICC, WB
Purity
Purified by Immunoaffinity chromatography.
Pricing
$1,075/0.1 mL | $4,825/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R2me1 antibody.)
Histone H3.1, Polyclonal Antibody (Cat# AAA6420743)
Full Name
Histone H3.1 (HIST1H3J, Histone H3R2me1, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l, HIST3H3) (AP)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K4me2 antibody.)
Histone H3.1, Polyclonal Antibody (Cat# AAA6420624)
Full Name
Histone H3.1 (HIST1H3J, Histone H3K4me2, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, _+E_Histone H3/k, Histone H3/l, HIST3H3) (Biotin)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC, IP, ChIP
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Immunoprecipitation (IP)
(5-Methylcytosine antibody (mAb) (Clone 33D3) tested by Methyl-DNA Immunoprecipitation (MeDIP). Human DNA (500 ng) digested with Mse I was subjected to MeDIP and then analyzed by quantitative real-time PCR with primers specific for genes that are normally methylated (Xist, NYESO and SNRPN) or unmethylated (APC and p48). The MeDIP'd DNA (enriched DNA) was plotted.)
5-Methylcytosine, Monoclonal Antibody (Cat# AAA388526)
Full Name
5-Methylcytosine (5-mC) antibody (mAb)(Clone 33D3)
Reactivity
Human
Applications
DB, EIA, ICC, IF, IHC, MeDIP
Purity
Protein A Chromatography
Pricing
$330/0.05 mg | $1,420/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R8me2a antibody.)
Histone H3.1, Polyclonal Antibody (Cat# AAA6420765)
Full Name
Histone H3.1 (HIST1H3J, Histone H3R8me2a, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l, HIST3H3) (AP)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R8me2s antibody.)
Histone H3.1, Polyclonal Antibody (Cat# AAA6420778)
Full Name
Histone H3.1 (HIST1H3J, Histone H3R8me2s, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l, HIST3H3) (Biotin)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IP
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB)
(MGMT Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).)
MGMT, Polyclonal Antibody (Cat# AAA6320676)
Full Name
MGMT, NT (MGMT, Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase) (MaxLight 650)
Reactivity
Human
Applications
FC/FACS, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R2me2s antibody.)
Histone H3.1, Polyclonal Antibody (Cat# AAA6420758)
Full Name
Histone H3.1 (HIST1H3J, Histone H3R2me2s, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, _+E_Histone H3/k, Histone H3/l, HIST3H3) (HRP)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC, IP, ChIP
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R17me1 antibody.)
Histone H3.1, Polyclonal Antibody (Cat# AAA6420525)
Full Name
Histone H3.1 (HIST1H3J, Histone H3, R17me1, H3/j,H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l) (Biotin)
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC, IP, ChIP
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K27me3 antibody.)
Histone H3.1, Polyclonal Antibody (Cat# AAA6420577)
Full Name
Histone H3.1 (HIST1H3J, Histone H3K27me3, H3/j, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l, HIST3H3) (PE)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC, IP, ChIP
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K9me2 antibody.)
Histone H3.1, Polyclonal Antibody (Cat# AAA6420681)
Full Name
Histone H3.1 (HIST1H3J, Histone H3K9me2, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, Histone H3/k, Histone H3/l, HIST3H3) (HRP)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC, IP, ChIP
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Dot Blot (DB)
(Dot blot analysis using MBS646539 on nitrocellulose membrane. 50ng of methylated peptide or non-methylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug/ml.)
H4, Polyclonal Antibody (Cat# AAA6305287)
Full Name
H4 (K20 [Me2]) (HIST1H4A, H4/A, H4FA, Histone H4) (FITC)
Gene Names
HIST2H4B; H4/o
Reactivity
Human
Applications
IHC, FC/FACS, WB, DB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Testing Data
(Representative blot from a previous lot. HeLa acid extract (lane 1 and lanes 3 through 7) and recombinant histone H3 (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS616986 H3 (Lys4) (0.5ug/ml; lanes 1 and 2) or probed with antibody at the same concentration pre-incubated with 1uM histone H3 peptides with the following modifications: Lane 3: monomethyl-lysine 4 Lane 4: dimethyl-lysine 4 Lane 5: trimethyl-lysine 4 Lane 6: monomethyl-lysine 9 Lane 7: monomethyl-lysine 27 Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates monomethyl-histone H3 (Lys4), (~17kD).)
Histone H3, Polyclonal Antibody (Cat# AAA6480583)
Full Name
Histone H3, monomethyl (Lys4) (Biotin)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human
Applications
WB, ChIP, ICC
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$1,075/0.1 mL | $4,825/5x0.1 mL
Dot Blot (DB)
(Dot blot analysis using MBS646539 on nitrocellulose membrane. 50ng of methylated peptide or non-methylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug/ml.)
H4, Polyclonal Antibody (Cat# AAA6305289)
Full Name
H4 (K20 [Me2]) (HIST1H4A, H4/A, H4FA, Histone H4) (MaxLight 405)
Gene Names
HIST2H4B; H4/o
Reactivity
Human
Applications
IHC, FC/FACS, WB, DB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
Western Blot (WB)
(Histone H3 monomethyl Lys18 pAb tested by Western blot. HEK293 nuclear extract (20 ug) probed with Histone H3 monomethyl Lys18 pAb (1:500 dilution).)
Histone H3K18me1, Polyclonal Antibody (Cat# AAA388533)
Full Name
Histone H3K18me1 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
DB, WB
Pricing
$200/0.01 mL | $590/0.1 mL | $2,595/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R26me2s antibody.)
Histone H3.1, Polyclonal Antibody (Cat# AAA6420742)
Full Name
Histone H3.1 (HIST1H3J, Histone H3R26me2s, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, _+E_Histone H3/k, Histone H3/l) (PE)
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
IF, WB
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Chromatin Immunoprecipitation (ChIP)
(AbFlex Histone H3K4me3 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of KMS11 cell chromatin and 4 ug of AbFlex Histone H3K4me3 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 19.8 million sequence tags were mapped to identify Histone H3K4me3 binding sites on chromosome 7.)
Histone H3K4me3, Antibody (Cat# AAA389489)
Full Name
AbFlex Histone H3K4me3 antibody (rAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, DB, WB
Purity
Protein A Chromatography
Pricing
$160/0.01 mg | $460/0.1 mg | $2,020/5x0.1 mg
Immunofluorescence (IF)
(Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) tested by immunofluorescence. Staining of HeLa cells with Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) (1:250 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).)
Histone H3K9me3, Monoclonal Antibody (Cat# AAA388443)
Full Name
Histone H3K9me3 antibody (mAb)(Clone 2AG-6F12-H4)
Reactivity
Human, Wide Range Predicted
Applications
DB, ICC, IF, WB
Purity
Culture Supernatant
Pricing
$220/0.01 mL | $610/0.1 mL | $2,685/5x0.1 mL
double-stranded RNA (J2), Monoclonal Antibody (Cat# AAA574014)
Full Name
Mouse anti double-stranded RNA (J2)
Reactivity
double-stranded RNA
Applications
EIA, IAC, ICC, IHC
Purity
Affinity chromatography on Protein A-agarose. Gel electrophoretically pure IgG antibody.
Pricing
$915/0.5 mg | $3,960/5x0.5 mg
double-stranded RNA (J2, J5 and K1), Monoclonal Antibody (Cat# AAA574021)
Full Name
Mouse anti double-stranded RNA (J2, J5 and K1) Comparison Kit
Reactivity
double-stranded RNA
Applications
EIA, DB, IAC, ICC, IHC
Purity
Affinity chromatography on Protein A-agarose. Gel electrophoretically pure IgG antibody.
Pricing
$3,120/15x0.1 mg | $725/3x0.1 mg
Immunoprecipitation (IP)
(5-Hydroxymethylcytidine antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 2 ?g of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 2 ?g of control rabbit IgG (IgG, blue bars). Real time quantitative PCR was performed on the immunoprecipitated DNA and results plotted as % of input DNA. The spiked APC DNA contained either no methylation (DNA), 5-methylcytosine methylation (5-mC) or 5-hydroxymethylcytosine methylation (5-hmC). The 5-hmC antibody shows a 650-fold enrichment for 5-hmC DNA compared to 5-mC DNA or unmethylated DNA.)
5-Hydroxymethylcytosine, Polyclonal Antibody (Cat# AAA388362)
Full Name
5-Hydroxymethylcytosine (5-hmC) antibody (pAb)
Reactivity
Human, Mouse
Applications
DB, IF, IHC, MeDIP
Purity
Protein A Chromatography
Pricing
$190/0.01 mg | $330/0.05 mg | $545/0.1 mg | $2,380/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3 trimethyl Lys9 antibody tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of Histone H3 trimethyl Lys9 pAb or the equivalent amount of rabbit IgG. RT-qPCR was performed on DNA purified from each of the ChIP DNA using a primer pair for the SAT2 alpha gene. Data are presented as Fold Enrichment of the antibody signal versus the negative control IgG using the ddCT method.)
Histone H3K9me3, Monoclonal Antibody (Cat# AAA388722)
Full Name
Histone H3K9me3 antibody (mAb)(Clone MABI 0319)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, DB, ICC, IF, WB
Purity
Protein G Chromatography
Pricing
$375/0.05 mg | $600/0.1 mg | $2,645/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3K79me2 antibody (pAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 3 million HeLa cells and 5 ul of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The images are characteristic of the binding patterns seen at many genes across the genome with the highest binding occurring at the promoter and a gradual decrease as signal moves into the gene body.)
Histone H3K79me2, Polyclonal Antibody (Cat# AAA388382)
Full Name
Histone H3K79me2 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, DB, WB
Pricing
$200/0.01 mL | $600/0.1 mL | $2,645/5x0.1 mL
pan Asymmetric-DiMethyl-Arginine, Polyclonal Antibody (Cat# AAA8578941)
Full Name
pan Asymmetric-DiMethyl-Arginine pAb
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
WB
Purity
Affinity purification
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Sample Data: Representative western blot of the di-methylated arginine protein profile with Anti-Methylated Arginine (NG, NG-Di-methyl) (1:1000) using human melanoma (MMRU) cell lysate (A) and the MMRU lysate plus additional di-methyl arginine peptide inhibitor (B).)
Methylated Arginine (NG, NG-Di-methyl), Polyclonal Antibody (Cat# AAA515856)
Full Name
Anti-Methylated Arginine (NG, NG-Di-methyl)
Reactivity
Pan-specific antibody
No cross-reaction with acetylated proteins, mono-methylated proteins.
No cross-reaction with acetylated proteins, mono-methylated proteins.
Applications
WB, EIA, IHC, IF
Purity
By affinity chromatography on a di-methyl arginine agarose column
Pricing
$435/0.1 mg | $1,735/5x0.1 mg
double-stranded RNA (K2), Monoclonal Antibody (Cat# AAA574018)
Full Name
Mouse anti double-stranded RNA (K2)
Reactivity
double-stranded RNA
Applications
EIA, IF
Pricing
$915/10 mL | $3,960/5x10 mL
double-stranded RNA (K2), Monoclonal Antibody (Cat# AAA574017)
Full Name
Mouse anti double-stranded RNA (K2)
Reactivity
double-stranded RNA
Applications
EIA, IF
Pricing
$610/5 mL | $2,585/5x5 mL
Dot Blot (DB)
(N6-Methyladenosine (m6A) antibody (pAb) tested by RNA dot blot analysis. 3.3ng of a 22 nt RNA containing four repeats of the GAC consensus for N6-methyladenosine within its sequence was spotted onto a membrane and probed with N6-Methyladenosine antibody (1 ug/ml). The sequence contained various adenosine modifications at all four positions as indicated. Lane 1: Adenosine. Lane 2: N6-methyladenosine. Lane 3: 1-methyladenosine. Lane 4: 2’-O-methyladenosine. Lane 5: inosine)
N6-Methyladenosine, Polyclonal Antibody (Cat# AAA388922)
Full Name
N6-Methyladenosine (m6A) antibody (pAb)
Reactivity
Not Species Specific
Applications
DB
Purity
Protein A Chromatography
Pricing
$200/0.01 mg | $360/0.05 mg | $590/0.1 mg | $2,595/5x0.1 mg
Immunoprecipitation (IP)
(5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 ?l of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 ?l of control rabbit IgG (IgG, blue bars). Real time quantitative PCR was performed on the immunoprecipitated DNA and results plotted as % of input DNA. The spiked APC DNA contained either no methylation (DNA), 5-methylcytosine methylation (5-mC) or 5-hydroxymethylcytosine methylation (5-hmC).)
5-Hydroxymethylcytosine, Polyclonal Antibody (Cat# AAA388354)
Full Name
5-Hydroxymethylcytosine (5-hmC) antibody (pAb)
Reactivity
Human, Mouse
Applications
DB, ICC, IF, IHC, MeDIP
Pricing
$190/0.01 mL | $330/0.05 mL | $545/0.1 mL | $2,380/5x0.1 mL
Immunofluorescence (IF)
(Histone H3 monomethyl Lys27 antibody tested by immunofluorescence. Top left: HeLa cells stained with Histone H3 monomethyl Lys27 antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.)
Histone H3K27me1, Polyclonal Antibody (Cat# AAA388464)
Full Name
Histone H3K27me1 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
DB, ICC, IF, WB
Pricing
$200/0.01 mL | $590/0.1 mL | $2,595/5x0.1 mL
Western Blot (WB)
(Western blot of Histone H3 dimethyl Lys36 antibody. HeLa nuclear extract (20 ug per lane) probed with H3K36me2 antibody (2 ug/ml dilution).)
Histone H3K36me2, Monoclonal Antibody (Cat# AAA388725)
Full Name
Histone H3K36me2 antibody (mAb)(Clone MABI 0332)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, DB, IF, WB
Purity
Protein G Chromatography
Pricing
$600/0.1 mg | $2,645/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3 dimethyl Lys9 antibody tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and 100 ul of Ready-to-ChIP HeLa Chromatin per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region 1 kb upstream of the human HOXD13 promoter was amplified by PCR. - Lane 1: ChIP using 10 ug of Histone H3 dimethyl Lys9 antibody. - Lane 2: PCR input control. - Lane 3: ChIP using negative control mouse IgG.)
Histone H3K9me2, Monoclonal Antibody (Cat# AAA388540)
Full Name
Histone H3K9me2 antibody (mAb)(Clone MABI 0307)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, DB, ICC, IF, WB
Purity
Protein G Chromatography
Pricing
$600/0.1 mg | $2,645/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3 monomethyl Lys79 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of Histone H3 monomethyl Lys79 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Histone H3K79me1, Polyclonal Antibody (Cat# AAA388633)
Full Name
Histone H3K79me1 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, DB, WB
Purity
Protein A Chromatography
Pricing
$200/0.01 mg | $600/0.1 mg | $2,645/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3 dimethyl Lys9 antibody tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of Histone H3 dimethyl Lys9 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for either the PABPC1 gene or the MyoD gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Histone H3K9me2, Polyclonal Antibody (Cat# AAA388463)
Full Name
Histone H3K9me2 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, DB, ICC, IF, IP, WB
Pricing
$200/0.01 mL | $600/0.1 mL | $2,645/5x0.1 mL
Dot Blot (DB)
(Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H3K23me3 antibody (pAb) for trimethyl-lysine 23 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with the antibody at a dilution of 1:10,000. The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9. Lane 9: unmodified Lys79. Lane 10: monomethyl Lys79. Lane 11: dimethyl Lys79. Lane 12: trimethyl Lys79. Bottom panel - Lane 1: Unmodified Lys23. Lane 2: Monomethyl Lys23. Lane 3: Dimethyl Lys23. Lane 4: Trimethyl Lys23. Lane 5: unmodified Lys27. Lane 6: monomethyl Lys27. Lane 7: dimethyl Lys27. Lane 8: trimethyl Lys27. Lane 9: unmodified Lys36. Lane 10: monomethyl Lys36. Lane 11: dimethyl Lys36. Lane 12: trimethyl Lys36.)
Histone H3K23me3, Polyclonal Antibody (Cat# AAA388924)
Full Name
Histone H3K23me3 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
DB, ICC, IF
Purity
Affinity Purified
Pricing
$200/0.01 mL | $590/0.1 mL | $2,595/5x0.1 mL
Western Blot (WB)
(Histone H3 dimethyl Lys23 pAb tested by Western blot. HEK293 nuclear extract (25 ug) was probed with Histone Histone H3 dimethyl Lys23 pAb (1:500 dilution).)
Histone H3K23me2, Polyclonal Antibody (Cat# AAA388528)
Full Name
Histone H3K23me2 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, DB, ICC, IF, WB
Pricing
$200/0.01 mL | $600/0.1 mL | $2,645/5x0.1 mL
Histone H3K27me3, Antibody (Cat# AAA389454)
Full Name
AbFlex Histone H3K27me3 antibody (rAb)
Reactivity
Human, Wide Range Predicted
Applications
DB, WB
Purity
Protein A Chromatography
Pricing
$160/0.01 mg | $460/0.1 mg | $2,020/5x0.1 mg
