Mouse CELF1 Monoclonal Antibody | anti-CELF1 antibody

CELF1 (CUGBP, Elav-like Family Member 1, CUGBP, NAB50, NAPOR, CUG-BP, CUGBP1, hNab50, BRUNOL2, EDEN-BP) (FITC)

Cat. Num. AAA6188060

• Gene Names: CELF1; CUGBP; NAB50; NAPOR; CUG-BP; CUGBP1; hNab50; BRUNOL2; EDEN-BP
• Reactivity: Human, Mouse, Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by Affinity Chromatography
• Synonyms: CELF1; Monoclonal Antibody; CELF1 (CUGBP; Elav-like Family Member 1; CUGBP; NAB50; NAPOR; CUG-BP; CUGBP1; hNab50; BRUNOL2; EDEN-BP) (FITC); anti-CELF1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 14i16
• Specificity: Recognizes CELF1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CELF1 antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: WB: 1:500-1:1000; IP: 1:20-1:50; Applications are based on unconjugated antibody.
• Immunogen: Synthetic methylated peptide of human CELF1.
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CELF1 antibody.)

Product Categories/Family for anti-CELF1 antibody

Antibodies; DEAD Box Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 71164871
• NCBI GeneID: 10658
• NCBI Accession #: NP_001020767.1
• NCBI GenBank Nucleotide #: NM_001025596.2
• UniProt Accession #: Q92879; Q4LE52; Q9NP83; Q9NR06; B4E2U5; D3DQS0; F8W940
• Molecular Weight: 52,063 Da
• NCBI Official Full Name: CUGBP Elav-like family member 1 isoform 3
• NCBI Official Synonym Full Names: CUGBP, Elav-like family member 1
• NCBI Official Symbol: CELF1
• NCBI Official Synonym Symbols: CUGBP; NAB50; NAPOR; CUG-BP; CUGBP1; hNab50; BRUNOL2; EDEN-BP
• NCBI Protein Information: CUGBP Elav-like family member 1; CELF-1; CUG-BP1; bruno-like 2; EDEN-BP homolog; bruno-like protein 2; CUG RNA-binding protein; deadenylation factor CUG-BP; RNA-binding protein BRUNOL-2; CUG-BP- and ETR-3-like factor 1; CUG triplet repeat RNA-binding prot
• UniProt Protein Name: CUGBP Elav-like family member 1
• Protein Family: CUGBP Elav-like family
• UniProt Gene Name: CELF1
• UniProt Synonym Gene Names: BRUNOL2; CUGBP; CUGBP1; NAB50; CELF-1; CUG-BP1
• UniProt Entry Name: CELF1_HUMAN

NCBI Description

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CELF1: RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre- mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver. Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. Belongs to the CELF/BRUNOL family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 11p11

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; ribonucleoprotein complex

Molecular Function: mRNA binding; protein binding; translation initiation factor binding; RNA binding; translation repressor activity, nucleic acid binding; BRE binding; nucleotide binding

Biological Process: mRNA splice site selection; embryonic development; regulation of RNA splicing; negative regulation of translation; positive regulation of multicellular organism growth; mRNA processing; spermatid development; germ cell development; RNA interference