Chromatin Immunoprecipitation (ChIP)
(Histone H3 monomethyl Lys79 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of Histone H3 monomethyl Lys79 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Rabbit Histone H3K79me1 Polyclonal Antibody | anti-H3K79me1 antibody
Histone H3K79me1 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot, Western Blot
Purity
Protein A Chromatography
Synonyms
Histone H3K79me1; Polyclonal Antibody; Histone H3K79me1 antibody (pAb); anti-H3K79me1 antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS (pH7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version of this antibody is also available.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3K79me1 antibody
Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 10ug per ChIP
WB: 1-2ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
ChIP: 10ug per ChIP
WB: 1-2ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Conjugation
Methylated
Immunogen
This Histone H3 monomethyl Lys79 antibody was raised against a peptide including monomethyl-lysine 79 of histone H3.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Histone H3 monomethyl Lys79 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of Histone H3 monomethyl Lys79 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Chromatin Immunoprecipitation (ChIP)
(Histone H3 monomethyl Lys79 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of Histone H3 monomethyl Lys79 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Western Blot (WB)
(Histone H3 monomethyl Lys79 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug) probed with Histone H3 monomethyl Lys79 antibody (1 ug/ml dilution).)
Western Blot (WB)
(Histone H3 monomethyl Lys79 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug) probed with Histone H3 monomethyl Lys79 antibody (1 ug/ml dilution).)
Dot Blot (DB)
(Histone H3 monomethyl Lys79 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys79 antibody for monomethyl-Lys79 of histone H3. Peptides corresponding to the region around lysine 79 of histone H3 were spotted onto PVDF and probed with Histone H3 monomethyl Lys79 antibody at a dilution of 1 ug/ml. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: unmodified Lys64 peptide. Lane 2: monomethyl Lys64 peptide. Lane 3: dimethyl Lys64 peptide. Lane 4: trimethyl Lys64 peptide. Lane 5: monomethyl Lys56 peptide. Lane 6: dimethyl Lys56 peptide. Lane 7: trimethyl Lys56 peptide. Lane 8: monomethyl Lys79 peptide. Lane 9: dimethyl Lys79 peptide. Lane 10: trimethyl Lys79 peptide. No detection of peptides (unmodified, mono-, di-, or tri-methylated) corresponding to lysine 4, lysine 9, lysine 18, lysine 23, lysine 27 and lysine 36 of Histone H3 was observed with Histone H3 dimethyl Lys79 antibody.)
Dot Blot (DB)
(Histone H3 monomethyl Lys79 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys79 antibody for monomethyl-Lys79 of histone H3. Peptides corresponding to the region around lysine 79 of histone H3 were spotted onto PVDF and probed with Histone H3 monomethyl Lys79 antibody at a dilution of 1 ug/ml. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: unmodified Lys64 peptide. Lane 2: monomethyl Lys64 peptide. Lane 3: dimethyl Lys64 peptide. Lane 4: trimethyl Lys64 peptide. Lane 5: monomethyl Lys56 peptide. Lane 6: dimethyl Lys56 peptide. Lane 7: trimethyl Lys56 peptide. Lane 8: monomethyl Lys79 peptide. Lane 9: dimethyl Lys79 peptide. Lane 10: trimethyl Lys79 peptide. No detection of peptides (unmodified, mono-, di-, or tri-methylated) corresponding to lysine 4, lysine 9, lysine 18, lysine 23, lysine 27 and lysine 36 of Histone H3 was observed with Histone H3 dimethyl Lys79 antibody.)
Related Product Information for anti-H3K79me1 antibody
Short Description: Our Histone H3K79me1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine 79 of histone H3 can be mono-, di- or trimethylated by Dot1 methylase; methylation at this residue acts as a marker of inactive chromatin regions that is critical for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine 79 of histone H3 can be mono-, di- or trimethylated by Dot1 methylase; methylation at this residue acts as a marker of inactive chromatin regions that is critical for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.
Product Categories/Family for anti-H3K79me1 antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3K79me1 (Catalog #AAA388633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3K79me1 antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3K79me1 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Western Blot (WB). Applications Validated: ChIP: 10ug per ChIP WB: 1-2ug/ml Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the H3K79me1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3K79me1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
