Mouse double-stranded RNA (K2) Monoclonal Antibody

Mouse anti double-stranded RNA (K2)

Cat. Num. AAA574017

• Reactivity: double-stranded RNA
• Applications: ELISA, Immunofluorescence
• Synonyms: double-stranded RNA (K2); Monoclonal Antibody; Mouse anti double-stranded RNA (K2); Mouse anti dsRNA; anti-double-stranded RNA (K2) antibody

• Host: Mouse
• Reactivity: double-stranded RNA
• Clonality: Monoclonal
• Isotype: IgM kappa
• Clone Number: K2
• Form/Format: Culture supernatant (RPMI, 5% fetal calf serum). Undiluted hybridoma supernatant.

• Applicable Applications for anti-double-stranded RNA (K2) antibody: ELISA (EIA), Immunofluorescence (IF)
• Application Notes: MAb K2 is primarily used for a sandwich ELISA to detect and quantitate (after calibration) dsRNA (see Schonborn et al.). For this application it should be diluted 1:2 with PBS. It may also be advantageous to use K2 for immunofluorescence studies.
• Source: Female DBA/2 mice were injected intraperitonially with a mixture of 50ug L-dsRNA and 75ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.
• Preparation and Storage: After delivery antibodies should be aliquoted and stored at -20 degree or -70 degree C. After adding 10mM sodium azide undiluted antibody can also be stored at +4 degree C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided.; The IgM mononclonal antibody K2 is only sold as hybridoma supernatant which is stored frozen and shipped on ice.

Related Product Information for anti-double-stranded RNA (K2) antibody

The mAb K2 recognizes double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp dsRNA. Recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (4050 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by K2. As described by Schonborn et al. K2 binds with high avidity to all dsRNAs investigated.
Background: Over the past decade our double-stranded RNA (dsRNA) antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. In addition, the anti-dsRNA antibodies can be used as a test tool to detect pathogens, including detection in paraffin-embedded fixed tissue samples (Richardson et al. 2010). K2 anti-dsRNA monoclonal antibody has an IgM isotype.
K2 is mainly used for specialised applications, where J2 and K1-antibodies that can be shipped at ambient temperature-do not suffice. Due to its IgM isotype K2 is more prone to aggregation at high concentration, and therefore cannot be lyophilized and needs to be shipped on ice.
K2 has primarily been used in ELISA and sandwich ELISA. Generally, K2 can be used in applications where an anti-dsRNA antibody with an isotype other than IgG (IgG2a) is required.

Product Categories/Family for anti-double-stranded RNA (K2) antibody

Primary antibodies, Microbiology

References

1) Schonborn, J., Oberstrass, J., Breyel, E., Tittgen, J., Schumacher, J. and Lukacs, N. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19, 2993-3000.
2) Lukacs, N. (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods 47, 255-272.
3) Lukacs, N. (1997) Detection of sense: antisense duplexes by structurespecific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford.
4) S. J. Richardson, A. Willcox, D. A. Hilton, S. Tauriainen, H. Hyoty, A. J. Bone, A. K. Foulis, N. G. Morgan. Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. J Clin Virol. (2010) 49(3); 180-5. doi: 10.1016/j.jcv.2010.07.015.
5) Levican-Asenjo J, Soto-Rifo R, Aguayo F, Gaggero A, Leon O. Salmon cells SHK-1 internalize infectious pancreatic necrosis virus by macropinocytosis. J Fish Dis. 2019 Jul;42(7):1035-1046. doi: 10.1111/jfd.13009.