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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Ubiquitin" - showing 550-600
Immunofluorescence (IF)
(MBS9606053 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
UBAP2L, Polyclonal Antibody (Cat# AAA9606053)
Full Name
UBAP2L Antibody
Gene Names
UBAP2L; NICE4; NICE-4
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis on RAW264.7 cell lysate using RNF14 Antibody. The lane on the left is treated with the antigen-specific peptide.)
RNF14, Polyclonal Antibody (Cat# AAA9600573)
Full Name
RNF14 Antibody
Gene Names
RNF14; ARA54; HFB30; TRIAD2; HRIHFB2038
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of GALNS using anti-GALNS antibody (MBS1750805).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat small intestine tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNS antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GALNS at approximately 58KD. The expected band size for GALNS is at 58KD. )
GALNS, Antibody (Cat# AAA1750805)
Full Name
Anti-GALNS Picoband antibody
Gene Names
GALNS; GAS; MPS4A; GalN6S; GALNAC6S
Reactivity
Reacts with: Human, Mouse, Rat
Applications
WB, EIA
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Porcine Brain Tissue; Lane3: Human U-87MG Cells; Lane4: Human A549 Cells.)
Ubiquitin Carboxyl Terminal Hydrolase L1, Antibody (Cat# AAA2027047)
Full Name
Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
Gene Names
UCHL1; NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
Reactivity
Human
Applications
WB, ICC, IHC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using UBE4A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
UBE4A, Polyclonal Antibody (Cat# AAA9125659)
Full Name
UBE4A Polyclonal Antibody
Gene Names
UBE4A; E4; UFD2; UBOX2
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using USP25 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
USP25, Polyclonal Antibody (Cat# AAA9128536)
Full Name
USP25 Polyclonal Antibody
Gene Names
USP25; USP21
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin (MBS210332) and visualised with Streptavidin : HRP (MBS238416))
HISTIDINE TAG, Monoclonal Antibody (Cat# AAA212127)
Full Name
MOUSE ANTI HISTIDINE TAG
Applications
EIA, IP, WB
Pricing
$265/0.1 mg | $1,035/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysate showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:1000.)
Ubiquitin, Polyclonal Antibody (Cat# AAA806802)
Full Name
Ubiquitin Antibody: Biotin
Reactivity
Human, Monkey, Mouse, Rat, Hamster, Rabbit, Guinea pig, Bovine, Pig, Canine, Sheep, Chicken, Xenopus, Yeast, Drosophila, Fish (Rainbow trout)
Applications
WB, IP, IF
Pricing
$520/0.2 mL | $2,130/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-UCHL1 / PGP9.5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
UCHL1 / PGP9.5, Polyclonal Antibody (Cat# AAA243689)
Full Name
Rabbit Polyclonal (IgG) to Human UCHL1 / PGP9.5
Gene Names
UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
Reactivity
Human, Mouse, Rat
Applications
IHC - Paraffin, WB, EIA
Purity
Immunoaffinity Purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Immunohistochemistry (IHC)
(MBS9603721 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
UBE2E2, Polyclonal Antibody (Cat# AAA9603721)
Full Name
UBE2E2 Antibody
Gene Names
UBE2E2; UBCH8
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of MIB1 using HeLa whole cell lysates)
MIB1, Polyclonal Antibody (Cat# AAA9608720)
Full Name
MIB1 Antibody
Gene Names
MIB1; MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9600649 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
BAP1, Polyclonal Antibody (Cat# AAA9600649)
Full Name
Phospho-BAP1 (Ser592) Antibody
Gene Names
BAP1; UCHL2; hucep-6; HUCEP-13
Reactivity
Human
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis of USP9X in lysates of HeLa)
USP9X, Polyclonal Antibody (Cat# AAA9603726)
Full Name
USP9X Antibody
Gene Names
USP9X; FAF; FAM; DFFRX; MRX99; MRXS99F
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells using UBR1 antibody.)
UBR1, Polyclonal Antibody (Cat# AAA9605485)
Full Name
UBR1 Antibody
Gene Names
UBR1; JBS
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of BRCA1 expression in serum treated COLO205 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
BRCA1, Polyclonal Antibody (Cat# AAA9601865)
Full Name
BRCA1 Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysates from SH-SY5Y cell line and mouse brain tissue (from left to right),using UCHL1 Antibody (C-term). MBS9212725 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
UCHL1, Polyclonal Antibody (Cat# AAA9212725)
Full Name
UCHL1 Antibody (C-term)
Gene Names
UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
Reactivity
Human, rat (Predicted Reactivity: Bovine, Monkey, Mouse, Pig)
Applications
EIA, IHC, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of USP18 using Jurkat whole lysates.)
USP18, Polyclonal Antibody (Cat# AAA9608470)
Full Name
USP18 Antibody
Gene Names
USP18; ISG43; UBP43; PTORCH2
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using BAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
BAP1, Polyclonal Antibody (Cat# AAA129835)
Full Name
BAP1 Polyclonal Antibody
Gene Names
BAP1; UCHL2; hucep-6; HUCEP-13
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis USP24 using Jurkat whole cell lysates)
USP24, Polyclonal Antibody (Cat# AAA9610299)
Full Name
USP24 Antibody
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of TDP43 using HT-29 whole cell lysates)
TDP43, Polyclonal Antibody (Cat# AAA9604433)
Full Name
TDP43 Antibody
Gene Names
TARDBP; ALS10; TDP-43
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Fig: 1 Western blot analysis of TRAF6 in normal human tissues using at 1: 2000. TRAF6 is observed at ~66 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF6 degradation fragments.)
TRAF-6, Polyclonal Antibody (Cat# AAA668820)
Full Name
Polyclonal Antibody to TRAF-6
Gene Names
TRAF6; RNF85; MGC:3310
Reactivity
Rat, Mouse, Human
Applications
IP, IHC, WB
Purity
Serum
Pricing
$300/0.05 mL | $1,280/5x0.05 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using UHRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
UHRF1, Polyclonal Antibody (Cat# AAA127391)
Full Name
UHRF1 Polyclonal Antibody
Gene Names
UHRF1; Np95; hNP95; ICBP90; RNF106; hUHRF1; huNp95
Reactivity
Human
Applications
WB, IHC, IF, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant RPN1 protein (red) or empty vector (blue) stained using RPN1 antibody)
RPN1, Monoclonal Antibody (Cat# AAA833396)
Full Name
RPN1 antibody
Gene Names
RPN1; OST1; RBPH1
Applications
FC/FACS, IHC, WB
Purity
RPN1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western blot detection of TBLR1 in Mouse brain, Mouse heart and K562 cell lysates using TBLR1 mouse mAb (1:1000 diluted).Predicted band size: 60KDa.Observed band size: 60Kda.)
TBLR1, Monoclonal Antibody (Cat# AAA475098)
Full Name
Anti-TBLR1 Mouse mAb
Gene Names
TBL1XR1; C21; DC42; IRA1; MRD41; TBLR1
Reactivity
Human, Mouse
Applications
WB, IHC, ICC
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Autocrine Motility Factor Receptor, Polyclonal Antibody (Cat# AAA2032152)
Full Name
Autocrine Motility Factor Receptor (AMFR) Polyclonal Antibody
Gene Names
Amfr; gp78
Reactivity
Mouse
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $210/0.2 mL | $355/0.5 mL | $435/1 mL | $1,240/5x1 mL
Immunohistochemistry (IHC)
(Rabbit Anti-LYN AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Appendix (Colon) TissueObserved Staining: Plasma membranePrimary Antibody Concentration: 1:600Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
LYN, Polyclonal Antibody (Cat# AAA3211808)
Full Name
LYN antibody - N-terminal region
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC)
(MBS9600736 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Skp2, Polyclonal Antibody (Cat# AAA9600736)
Full Name
Phospho-Skp2 (Ser64) Antibody
Gene Names
SKP2; p45; FBL1; FLB1; FBXL1
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Immunohistochemistry (IHC)
(Sample Type :Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :Ywhae: Red DAPI:BlueGene Name :YwhaeSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science)
Ywhae, Polyclonal Antibody (Cat# AAA3200747)
Full Name
Ywhae antibody - C-terminal region
Gene Names
Ywhae; AU019196
Reactivity
Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC)
(MBS9608015 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
UBE2C, Polyclonal Antibody (Cat# AAA9608015)
Full Name
UBE2C Antibody
Gene Names
UBE2C; UBCH10; dJ447F3.2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysates from HL-60,K562 cell line (from left to right), using ITGB2 Antibody MBS9231826. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
ITGB2, Monoclonal Antibody (Cat# AAA9231826)
Full Name
ITGB2 Antibody
Reactivity
Human
Applications
WB, IHC-P, EIA
Pricing
$445/0.1 mL | $1,695/5x0.1 mL
Western Blot (WB)
(Western blot-MKRN3 Polyclonal Antibody)
MKRN3, Polyclonal Antibody (Cat# AAA9140699)
Full Name
MKRN3 Polyclonal Antibody
Gene Names
MKRN3; CPPB2; D15S9; RNF63; ZFP127; ZNF127
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Smurf1 using HuvEc whole lysates.)
Smurf1, Polyclonal Antibody (Cat# AAA9608318)
Full Name
Smurf1 Antibody
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis UBA52 using COLO205 whole cell lysates)
UBA52, Polyclonal Antibody (Cat# AAA9608503)
Full Name
UBA52 Antibody
Gene Names
UBA52; L40; CEP52; RPL40; HUBCEP52
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Recommended SQSTM1 Antibody Titration: 0.2-1 ug/ml)
Sequestosome 1, Polyclonal Antibody (Cat# AAA5300080)
Full Name
Sequestosome 1 antibody
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purified
Pricing
$605/0.1 mL | $2,710/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from HT29 cells untreated or treated with UV using c-Cbl(phospho-Tyr700) Antibody.)
c-Cbl, Polyclonal Antibody (Cat# AAA9413544)
Full Name
c-Cbl (phospho-Tyr700) Antibody
Gene Names
CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
Reactivity
Human
Applications
WB, IHC, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Immunohistochemistry (IHC)
(Rabbit Anti-HNRNPU AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Testis TissueObserved Staining: Cytoplasm and nucleus in spermatogonia and spermatocytesPrimary Antibody Concentration: N/AOther Working Concentrations: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
HNRNPU, Polyclonal Antibody (Cat# AAA3205292)
Full Name
HNRNPU antibody - N-terminal region
Gene Names
HNRNPU; SAFA; HNRPU; SAF-A; U21.1; pp120; EIEE54; GRIP120; hnRNP U; HNRNPU-AS1
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat
Applications
WB, IHC
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 47.7 kDa SQSTM1 protein using Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 47.7 kDa. Other Band(s): ~60 in Jurkat.)
SQSTM1, Polyclonal Antibody (Cat# AAA809653)
Full Name
SQSTM1 Antibody: ATTO 390
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Human; Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB)
(Western blot analysis of CBL expression in Na2VO3 treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
CBL, Polyclonal Antibody (Cat# AAA9601822)
Full Name
CBL Antibody
Gene Names
CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9605363 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CNOT4, Polyclonal Antibody (Cat# AAA9605363)
Full Name
CNOT4 Antibody
Gene Names
CNOT4; NOT4; NOT4H; CLONE243
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(Figure. The apoptosis of A549 cells by TNFalpha was inhibited by TNFRSF1B.Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFalpha). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. TNFRSF1B can inhibit cell apoptosis which induced by TNFalpha. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1mug/mL TNFalpha and various concentrations of recombinant human TNFRSF1B. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate at 37 degree C for 1-4 hours. Apoptosis of A549 cells had been inhibit after incubation with TNFRSF1B for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant TNFRSF1B for 96h. The result was shown in Figure 2. It was obvious that TNFRSF1B significantly suppress cell apoptosis induced by TNFalpha.(A) A549 cells cultured in DMEM contain 1mug/mL TNFalpha and 0.1ng/mL TNFRSF1B for 96h; (B) A549 cells cultured in DMEM only contain 1mug/mL TNFalpha for 96h.)
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A), Active Protein (Cat# AAA2105140)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)
Gene Names
TNFRSF1A; FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$185/0.01 mg | $365/0.05 mg | $560/0.1 mg | $685/0.2 mg | $1,330/0.5 mg | $1,975/1 mg | $4,855/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (MBS1750600).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 22RV1 whole cell lysates,Lane 3: human A431 whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: human HepG2 whole cell lysates,Lane 6: human SGC-7901 whole cell lysates,Lane 7: human A549 whole cell lysates,Lane 8: rat brain tissue lysates,Lane 9: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAUSP/USP7 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HAUSP/USP7 at approximately 140KD. The expected band size for HAUSP/USP7 is at 128KD. )
HAUSP/USP7, Polyclonal Antibody (Cat# AAA1750600)
Full Name
Anti-HAUSP/USP7 Picoband antibody
Gene Names
USP7; TEF1; HAUSP
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(MBS9604481 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
TRI18, Polyclonal Antibody (Cat# AAA9604481)
Full Name
TRI18 Antibody
Gene Names
MID1; OS; FXY; OSX; OGS1; XPRF; BBBG1; GBBB1; MIDIN; RNF59; ZNFXY; TRIM18
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis USP50 using A549 whole cell lysates)
USP50, Polyclonal Antibody (Cat# AAA9602268)
Full Name
USP50 Antibody
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using RNF167 Antibody.)
RNF167, Polyclonal Antibody (Cat# AAA9609806)
Full Name
RNF167 Antibody
Gene Names
RNF167; LP2254; RING105; 5730408C10Rik
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data
(Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin (MBS210332) and visualised with Streptavidin : HRP (MBS238416))
HISTIDINE TAG, Monoclonal Antibody (Cat# AAA210678)
Full Name
MOUSE ANTI HISTIDINE TAG:FITC
Applications
FC/FACS, IF
Pricing
$430/0.1 mg | $1,790/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using Uba5 Antibody.)
Uba5, Polyclonal Antibody (Cat# AAA9602263)
Full Name
Uba5 Antibody
Gene Names
UBA5; EIEE44; SCAR24; THIFP1; UBE1DC1
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from A431 cells, using E1 Ubiquitin Activating Enzyme Antibody. The lane on the left was treated with blocking peptide.)
E1 Ubiquitin Activating Enzyme, Polyclonal Antibody (Cat# AAA9612389)
Full Name
E1 Ubiquitin Activating Enzyme Antibody
Gene Names
UBA1; A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of HepG2, using USP7 Antibody. The lane on the left is treated with the antigen-specific peptide.)
USP7, Polyclonal Antibody (Cat# AAA9607584)
Full Name
USP7 Antibody
Gene Names
USP7; TEF1; HAUSP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9214893 APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
APG8a/b (MAP1LC3A/B), Polyclonal Antibody (Cat# AAA9214893)
Full Name
APG8a/b (MAP1LC3A/B) Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
IF, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RNF220 antibody (MBS9140561) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
RNF220, Polyclonal Antibody (Cat# AAA9140561)
Full Name
RNF220 Polyclonal Antibody
Gene Names
RNF220; C1orf164
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
