Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Menin (MEN1) Standard Curve.)
Rat Menin (MEN1) ELISA Kit | MEN1 elisa kit
Rat Menin (MEN1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Menin (MEN1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Menin (MEN1) Standard Curve.)
Background: MEN1 encodes menin, a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of this protein is not known. Two messages have been detected on northern blots but the larger message has not been characterized. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI Description
displays conservation with the human and mouse homologs for amino acid residues that are mutated in human multiple endocrine neoplasia type 1 [RGD, Feb 2006]
Uniprot Description
MEN1: Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression. May be involved in DNA repair. Component of MLL-containing complexes (named MLL, ASCOM, MLL2/MLL3 or MLL3/MLL4 complex): at least composed ASH2L, RBBP5, DPY30, WDR5, one or several histone methyltransferases (MLL, MLL2, MLL3 and/or MLL4), and the facultative components MEN1, HCFC1, HCFC2, NCOA6, KDM6A, PAXIP1/PTIP and C16orf53/PA1. Interacts with POLR2A phosphorylated at 'Ser-5', but not with the unphosphorylated, nor 'Ser-2' phosphorylated POLR2A forms. Interacts with FANCD2 and DBF4. Interacts with JUND. Interacts with SMAD3, but not with SMAD2, nor SMAD4. Directly interacts with NFKB1, NFKB2 and RELA. Ubiquitous. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 1q43
Cellular Component: chromatin; cleavage furrow; cytoplasm; cytosol; histone methyltransferase complex; nuclear chromatin; nuclear chromosome, telomeric region; nuclear matrix; nucleoplasm; nucleus; protein complex
Molecular Function: chromatin binding; DNA binding; double-stranded DNA binding; four-way junction DNA binding; histone-lysine N-methyltransferase activity; protein binding, bridging; protein N-terminus binding; sequence-specific DNA binding; Y-form DNA binding
Biological Process: brain development; cell cycle arrest; chromatin remodeling; decidualization; embryonic skeletal morphogenesis; hemopoiesis; histone methylation; leukocyte homeostasis; MAPKKK cascade; maternal process involved in pregnancy; mitotic cell cycle; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein kinase activity; negative regulation of epithelial cell proliferation; negative regulation of JNK cascade; negative regulation of organ growth; negative regulation of osteoblast differentiation; negative regulation of protein amino acid phosphorylation; negative regulation of telomerase activity; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; ossification; osteoblast development; osteoblast fate commitment; palate development; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of cell differentiation; positive regulation of cell division; positive regulation of histone methylation; positive regulation of osteoblast differentiation; positive regulation of protein binding; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of activin receptor signaling pathway; regulation of gene expression; response to DNA damage stimulus; response to gamma radiation; response to UV
Research Articles on MEN1
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Product Notes
The Rat MEN1 men1 (Catalog #AAA9712296) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712296 ELISA Kit recognizes Rat MEN1. It is sometimes possible for the material contained within the vial of "Menin (MEN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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