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106 results for "cytoplasmic-export-protein" - showing 1-50


Immunofluorescence (IF) (MBS9606751 staining A2780 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

p27, Polyclonal Antibody (Cat# AAA9606751)

Full Name
p27 Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of RAN Antibody (N-term) in HL-60 cell line lysates (35ug/lane).RAN (arrow) was detected using the purified Pab.)

RAN, Polyclonal Antibody (Cat# AAA9210295)

Full Name
RAN Antibody (N-term)
Gene Names
RAN; TC4; Gsp1; ARA24
Reactivity
Human, mouse (Predicted Reactivity: Yeast, Bovine, C Elegans, Chicken, Zebrafish, Monkey, Rat, Xenopus)
Applications
WB, EIA, IHC-p, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with FMR1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

FMR1, Polyclonal Antibody (Cat# AAA9207834)

Full Name
FMR1 Antibody (N-term)
Gene Names
FMR1; POF; FMRP; POF1; FRAXA
Reactivity
Human, mouse
Applications
IHC-P, WB, FC,E
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (MBS9607093 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IGF2BP1, Polyclonal Antibody (Cat# AAA9607093)

Full Name
IGF2BP1 Antibody
Gene Names
IGF2BP1; IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of SLC8A1 Antibody (Center) in HL-60(lane 1), K562(lane 2) cell line lysates (35ug/lane). SLC8A1 (arrow) was detected using the purified Pab.)

SLC8A1, Polyclonal Antibody (Cat# AAA9215051)

Full Name
SLC8A1 Antibody (Center)
Gene Names
SLC8A1; NCX1
Reactivity
Human, mouse
Predicted Reactivity: Bovine, Rat
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis on HeLa cell lysate using NXF1 Antibody, The lane on the left is treated with the antigen-specific peptide.)

NXF1, Polyclonal Antibody (Cat# AAA9600306)

Full Name
NXF1 Antibody
Gene Names
NXF1; TAP; MEX67
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of DMP1 using COS7 whole cell lysates)

DMP1, Polyclonal Antibody (Cat# AAA9609142)

Full Name
DMP1 Antibody
Gene Names
DMP1; ARHP; ARHR; DMP-1
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of eIF5A2 using Jurkat whole cell lysates)

eIF5A2, Polyclonal Antibody (Cat# AAA9608855)

Full Name
eIF5A2 Antibody
Gene Names
EIF5A2; EIF-5A2; eIF5AII
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Pancreatic Carcinoma stained with MRP3 Mouse Monoclonal Antibody (ABCC3/2971).)

MRP3 (Multidrug Resistance-Associated Protein 3), Monoclonal Antibody (Cat# AAA4381363)

Full Name
MRP3 (Multidrug Resistance-Associated Protein 3)
Gene Names
ABCC3; MLP2; MRP3; ABC31; MOAT-D; cMOAT2; EST90757
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western blot analysis of Jurkat whole cell lysates, using EIF5A Antibody. The lane on the left is treated with the antigen-specific peptide.)

EIF5A, Polyclonal Antibody (Cat# AAA9607410)

Full Name
EIF5A Antibody
Gene Names
EIF5A; EIF-5A; EIF5A1; eIF5AI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (EIF5AL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF5AL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

EIF5AL1, Polyclonal Antibody (Cat# AAA9213433)

Full Name
EIF5AL1 Antibody (C-term)
Gene Names
EIF5AL1; EIF5AP1; bA342M3.3
Reactivity
Human
Applications
EIA, IHC, WB, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (The anti-Phospho-p27Kip1-T187Pab is used in Western blot to detect Phospho-p27Kip1-T187 in HL60 tissue lysate)

Phospho-p27Kip1 (T187), Polyclonal Antibody (Cat# AAA9200928)

Full Name
Phospho-p27Kip1 (T187) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human (Predicted Reactivity: Hamster)
Applications
WB, EIA, IHC-P
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (All lanes : Anti-CBP20 Antibody at 1:1000 dilutionLane 1: human heart lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

CBP20, Polyclonal Antibody (Cat# AAA9215924)

Full Name
CBP20 Antibody
Gene Names
NCBP2; CBC2; NIP1; CBP20; PIG55
Reactivity
Human, Mouse
Applications
WB
Pricing
$405/0.1 mL | $1,585/5x0.1 mL
Western Blot (WB) (HSF1 Antibody western blot analysis in Jurkat cell line and mouse heart tissue lysates (35ug/lane).This demonstrates the HSF1 antibody detected the HSF1 protein (arrow).)

HSF1 Sumoylation Site, Polyclonal Antibody (Cat# AAA9211638)

Full Name
HSF1 Sumoylation Site Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
IF, EIA, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysate from MCF-7 cell line, using CDKN1B-Y88. MBS9211878 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

CDKN1B-Y88, Polyclonal Antibody (Cat# AAA9211878)

Full Name
CDKN1B-Y88 Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human
Predicted Reactivity: Hamster
Applications
WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL

Cytoplasmic export protein 1 (CEX1), Recombinant Protein (Cat# AAA1457864)

Full Name
Recombinant Saccharomyces cerevisiae Cytoplasmic export protein 1 (CEX1) , partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.

ArpT2, Polyclonal Antibody (Cat# AAA833933)

Full Name
ArpT2 antibody
Gene Names
ACTRT2; ARPM2; ARPT2; Arp-T2; HARPM2
Applications
IHC, WB
Pricing
$730/0.1 mL | $3,270/5x0.1 mL

Acriflavine resistance protein E (acrE), Recombinant Protein (Cat# AAA1221281)

Full Name
Recombinant Escherichia coli Acriflavine resistance protein E (acrE)
Gene Names
acrE; ECK3252; envC; JW3233
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,920/1 mg (E-Coli) | $2,310/1 mg (Yeast) | $3,455/1 mg (Baculovirus) | $1,765/0.1 mg (Baculovirus) | $2,355/0.1 mg (Mammalian-Cell) | $1,070/0.1 mg (E-Coli) | $1,160/0.1 mg (Yeast) | $5,195/0.5 mg (Mammalian-Cell) | $1,225/0.02 mg (Baculovirus) | $1,490/0.02 mg (Mammalian-Cell) | $890/0.02 mg (E-Coli) | $1,020/0.02 mg (Yeast)

Nucleoporin gle1 (gle1), Recombinant Protein (Cat# AAA1037478)

Full Name
Recombinant Schizosaccharomyces pombe Nucleoporin gle1 (gle1)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$2,185/1 mg (E-Coli) | $2,570/1 mg (Yeast) | $3,745/1 mg (Baculovirus) | $1,925/0.1 mg (Baculovirus) | $2,520/0.1 mg (Mammalian-Cell) | $1,210/0.1 mg (E-Coli) | $1,290/0.1 mg (Yeast) | $6,205/0.5 mg (Mammalian-Cell) | $1,335/0.02 mg (Baculovirus) | $1,600/0.02 mg (Mammalian-Cell) | $1,035/0.02 mg (E-Coli) | $1,130/0.02 mg (Yeast)
Western Blot (WB) (Dilution: WB - 1:4000All lanes: Anti-SCAP Antibody (N-term) at 1:4000 dilution Lane 1: HT-29 whole cell lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 140 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

SCAP, Monoclonal Antibody (Cat# AAA9231325)

Full Name
SCAP Antibody (N-term)
Reactivity
Human
Applications
WB, EIA
Purity
Purified through a protein G column, followed by dialysis against PBS.
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB) (Western blot detection of SirT2 in PC-12, Rat Brain and Mouse Brain cell lysates using SirT2 mouse mAb (1:1000 diluted). Predicted band size: 39,43KDa. Observed band size:39,43KDa.)

SirT2, Monoclonal Antibody (Cat# AAA9231168)

Full Name
Anti-SirT2 Antibody
Gene Names
SIRT2; SIR2; SIR2L; SIR2L2
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Pricing
$415/0.1 mL | $1,625/5x0.1 mL

Polyadenylate-binding protein, cytoplasmic and nuclear (pab1), Recombinant Protein (Cat# AAA1137761)

Full Name
Recombinant Schizosaccharomyces pombe Polyadenylate-binding protein, cytoplasmic and nuclear (pab1), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Western Blot (WB) (SLC8A3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SLC8A3 antibody detected the SLC8A3 protein (arrow).)

SLC8A3, Polyclonal Antibody (Cat# AAA9206025)

Full Name
SLC8A3 Antibody (C-term)
Gene Names
SLC8A3; NCX3
Reactivity
Human
Applications
WB, EIA, IHC-p
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL

CHK1 (S280), Blocking Peptide (Cat# AAA9224628)

Full Name
CHK1 Antibody (S280) Blocking Peptide
Gene Names
CHEK1; CHK1
Immunohistochemistry (IHC) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

HSF1, Polyclonal Antibody (Cat# AAA9612889)

Full Name
Phospho-HSF1 (Ser326) Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL

CHK1 (S317), Blocking Peptide (Cat# AAA9225010)

Full Name
Phospho-CHK1(S317) Antibody Blocking peptide
Gene Names
CHEK1; CHK1

EGLN1, Blocking Peptide (Cat# AAA9223617)

Full Name
EGLN1 Antibody (C-term) Blocking Peptide
Gene Names
EGLN1; HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2

EIF5AL1, Blocking Peptide (Cat# AAA9218778)

Full Name
EIF5AL1 Antibody (C-term) Blocking peptide
Gene Names
EIF5AL1; EIF5AP1; bA342M3.3

LAS1L, Blocking Peptide (Cat# AAA9222498)

Full Name
LAS1L Antibody (C-term) Blocking Peptide
Gene Names
LAS1L; Las1-like; dJ475B7.2

DMP1, Blocking Peptide (Cat# AAA9223897)

Full Name
DMP1 Antibody (N-term) Blocking Peptide
Gene Names
DMP1; ARHP; ARHR; DMP-1
Western Blot (WB) (Western blot analysis SREBP-2 (Phospho-Ser455) using HepG2 whole cell lysates.-/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).)

SREBP2, Polyclonal Antibody (Cat# AAA9614321)

Full Name
Phospho-SREBP2 (Ser455) Antibody
Gene Names
SREBF2; SREBP2; bHLHd2; SREBP-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (90%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL

MEX3B, Blocking Peptide (Cat# AAA9222022)

Full Name
MEX3B Antibody (N-term) Blocking Peptide
Gene Names
MEX3B; RKHD3; MEX-3B; RNF195

NXF1, Blocking Peptide (Cat# AAA9220872)

Full Name
NXF1 Antibody (N-term) Blocking Peptide
Gene Names
NXF1; TAP; MEX67

CHEK1, Blocking Peptide (Cat# AAA9229068)

Full Name
CHEK1 Blocking Peptide (Center S296)
Gene Names
CHEK1; CHK1
Western Blot (WB) (Western blot analysis of Chk1 in (1) K562 cell lysate; (2) PC-12 cell lysate (MBS179540).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHEK1 )

Chk1, Monoclonal Antibody (Cat# AAA179540)

Full Name
Anti-Chk1 Rabbit Monoclonal Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Phospho-Chk1 (S296) expression in HEK293 cell lysate Treated with Calyculin (MBS1750252).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHEK1 )

Chk1, Monoclonal Antibody (Cat# AAA1750252)

Full Name
Anti-Phospho-Chk1 (S296) Rabbit Monoclonal Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

CHK1 (S280), Blocking Peptide (Cat# AAA9225009)

Full Name
Phospho-CHK1(S280) Antibody Blocking peptide
Gene Names
CHEK1; CHK1
Western Blot (WB) (Western blot analysis of extracts from various samples, using DDX3 Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: Rat brain,Lane 4: HepG2 cells(heat shock treatment),Lane 5: Mouse muscle.)

DDX3, Polyclonal Antibody (Cat# AAA9613803)

Full Name
DDX3 Antibody
Gene Names
DDX3X; DBX; DDX3; HLP2; DDX14
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

EIF5A2, Blocking Peptide (Cat# AAA9220416)

Full Name
EIF5A2 Antibody (C-term) Blocking peptide
Gene Names
EIF5A2; EIF-5A2; eIF5AII

CHK1, Blocking Peptide (Cat# AAA9226858)

Full Name
CHK1 Antibody (C-term) Blocking Peptide
Gene Names
CHEK1; CHK1
Western Blot (WB) (Figure 1. Western blot analysis of Chk1 using anti- Chk1 antibody (MBS1750555).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: SW620 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Chk1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Chk1 at approximately 54KD. The expected band size for Chk1 is at 54KD. )

Chk1, Polyclonal Antibody (Cat# AAA1750555)

Full Name
Anti-Chk1 Picoband Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of RanBP2 using anti-RanBP2 antibody (MBS1750530). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RanBP2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RanBP2 at approximately 270KD. The expected band size for RanBP2 is at 358KD. )

RanBP2, Polyclonal Antibody (Cat# AAA1750530)

Full Name
Anti-RanBP2 Picoband Antibody
Gene Names
RANBP2; ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis NFAT1 (Phospho-Ser326) using Rat brain tissue lysates.-/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).)

NFAT1, Polyclonal Antibody (Cat# AAA9614012)

Full Name
Phospho-NFAT1 (Ser326) Antibody
Gene Names
NFATC2; NFAT1; NFATP
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (90%), Xenopus (85%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of Chk1 expression in HeLa cell lysate (MBS179539).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHEK1 )

Chk1, Monoclonal Antibody (Cat# AAA179539)

Full Name
Anti-Chk1 Rabbit Monoclonal Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

HSF1 / Heat Shock Factor Protein 1, Recombinant Protein (Cat# AAA2901809)

Full Name
Human HSF1 / Heat Shock Factor Protein 1 Recombinant Protein
Gene Names
HSF1; HSTF1
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Immunofluorescence (IF)/Immunocytochemistry (ICC) (Staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6365 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Smad3, Polyclonal Antibody (Cat# AAA9613520)

Full Name
Smad3 Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (86%)
Applications
IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from Hela cells, using Phospho-P62/SQSTM1(Ser28) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

SQSTM1/p62, Polyclonal Antibody (Cat# AAA9614520)

Full Name
Phospho-SQSTM1/p62 (Ser28) Antibody
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (83%), Bovine (83%), Rabbit (83%)
Applications
WB, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis Smad3 (Phospho-Ser423) using HepG2 whole cell lysates.-/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).)

Smad3, Polyclonal Antibody (Cat# AAA9614448)

Full Name
Phospho-Smad3 (Ser423) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunohistochemistry (IHC) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

HSF1, Polyclonal Antibody (Cat# AAA9612695)

Full Name
Phospho-HSF1 (Ser121) Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from rat brain, using p27 Kip1 (Phospho-Ser178) Antibody. Lane 1 was treated with the blocking peptide.)

p27 Kip1, Polyclonal Antibody (Cat# AAA9614053)

Full Name
Phospho-p27 Kip1 (Ser178) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
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