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Immunohistochemistry (IHC) (EIF5AL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF5AL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Rabbit anti-Human EIF5AL1 Polyclonal Antibody | anti-EIF5AL1 antibody

EIF5AL1 Antibody (C-term)

Gene Names
EIF5AL1; EIF5AP1; bA342M3.3
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EIF5AL1; Polyclonal Antibody; EIF5AL1 Antibody (C-term); Eukaryotic translation initiation factor 5A-1-like; eIF-5A-1-like; eIF-5A1-like; Eukaryotic initiation factor 5A isoform 1-like; anti-EIF5AL1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EIF5AL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the C-terminal region of human EIF5AL1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
94-122
Sequence Length
154
Applicable Applications for anti-EIF5AL1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF)
Application Notes
IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Function
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).
Cellular Location
Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(EIF5AL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF5AL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (EIF5AL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF5AL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(EIF5AL1 Antibody (C-term) western blot analysis in Ramos,A2058 cell line lysates (35ug/lane).This demonstrates the EIF5AL1 antibody detected the EIF5AL1 protein (arrow).)

Western Blot (WB) (EIF5AL1 Antibody (C-term) western blot analysis in Ramos,A2058 cell line lysates (35ug/lane).This demonstrates the EIF5AL1 antibody detected the EIF5AL1 protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of EIF5AL1 Antibody (C-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of EIF5AL1 Antibody (C-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-EIF5AL1 antibody
EIF5AL1is a mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).
References
Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
Clement, P.M., et al. FEBS J. 273(6):1102-1114(2006)
Facchiano, A.M., et al. Protein Eng. 14(11):881-890(2001)
Lipowsky, G., et al. EMBO J. 19(16):4362-4371(2000)
Koettnitz, K., et al. Gene 159(2):283-284(1995)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16773
NCBI Official Full Name
eukaryotic translation initiation factor 5A-1-like
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 5A-like 1
NCBI Official Symbol
EIF5AL1
NCBI Official Synonym Symbols
EIF5AP1; bA342M3.3
NCBI Protein Information
eukaryotic translation initiation factor 5A-1-like
UniProt Protein Name
Eukaryotic translation initiation factor 5A-1-like
UniProt Gene Name
EIF5AL1
UniProt Synonym Gene Names
eIF-5A-1-like; eIF-5A1-like
UniProt Entry Name
IF5AL_HUMAN

Uniprot Description

EIF5AL1: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Belongs to the eIF-5A family.

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: endoplasmic reticulum membrane; nuclear pore

Molecular Function: ribosome binding; translation elongation factor activity

Biological Process: protein transport; mRNA transport; translational frameshifting; positive regulation of translational elongation; positive regulation of translational termination

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Product Notes

The EIF5AL1 eif5al1 (Catalog #AAA9213433) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 94-122. The EIF5AL1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF5AL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF). IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50. Researchers should empirically determine the suitability of the EIF5AL1 eif5al1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF5AL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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