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956 results for " Calpain Antibody" - showing 1-50
Western Blot (WB)
(Western blotAll lanes: CAPN2 antibody at 15ug/mlLane 1: Mouse lung tissueLane 2: 3T3 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: A431 whole cell lysateLane 5: Hela whole cell lysateLane 6: A549 whole cell lysateLane 7: HepG-2 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 80,72 kDaObserved band size: 80,42 kDa)
Calpain-2 catalytic subunit, Polyclonal Antibody (Cat# AAA1491556)
Full Name
Rabbit anti-human Calpain-2 catalytic subunit polyclonal Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western Blot: Sample: Human HCT116 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human CAPN9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Calpain 9, Polyclonal Antibody (Cat# AAA2033864)
Full Name
Polyclonal Antibody to Calpain 9 (CAPN9)
Gene Names
CAPN9; GC36; nCL-4
Reactivity
Human
Applications
WB, ICC, IHC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL
Western Blot (WB)
(Western blotAll lanes: CAPN1 antibody at 6ug/mlLane 1: A375 whole cell lysateLane 2: 293T whole cell lysateLane 3: PC-3 whole cell lysateLane 4: Jurkat whole cell lysateLane 5: Hela whole cell lysateLane 6: A2780 whole cell lysateLane 7: A431 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 82 kDaObserved band size: 82 kDa)
Calpain-1 catalytic subunit, Polyclonal Antibody (Cat# AAA1493523)
Full Name
Rabbit anti-human Calpain-1 catalytic subunit polyclonal Antibody(CAPN1)
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blotAll lanes: Calpastatin antibody at 10ug/mlLane 1: K562 whole cell lysateLane 2: Hela whole cell lysateLane 3: A549 whole cell lysateLane 4: HepG-2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 77,76,64,72,82,85,83,75,81 kDaObserved band size: 77 kDa)
Calpastatin, Polyclonal Antibody (Cat# AAA7002669)
Full Name
Rabbit anti-human Calpastatin polyclonal Antibody
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(All lanes: Calpain-1 catalytic subunit antibody at 2ug/mlLane 1:A431 whole cell lysateLane 2:Hela whole cell lysateLane 3:Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 82kDaObserved band size: 82kDa)
Calpain-1 catalytic subunit, Polyclonal Antibody (Cat# AAA1491955)
Full Name
Rabbit anti-human Calpain-1 catalytic subunit polyclonal Antibody(CAPN1)
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of Human normal liver and Mouse liver tissue, Mouse intestines and pancreas tissue, hepg2 and HT-29 cell, Mouse heart tissue using CAPN7 Polyclonal Antibody at dilution of 1:200)
CAPN7, Polyclonal Antibody (Cat# AAA2527313)
Full Name
CAPN7 Polyclonal Antibody
Gene Names
CAPN7; PALBH; CALPAIN7
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL
Western Blot (WB)
(Western blotAll lanes: CAPNS1antibody at 1.15ug/mlLane 1 : Mouse lung tissueLane 2 : Mouse brain tissueLane 3 : Hela whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 28 kDaObserved band size: 28 kDa)
CAPNS1, Polyclonal Antibody (Cat# AAA7044855)
Full Name
CAPNS1 Antibody
Gene Names
CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS7051813 at dilution of 1:100)
CAPN14, Polyclonal Antibody (Cat# AAA7051813)
Full Name
CAPN14 Antibody
Reactivity
Human
Applications
EIA, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blotAll lanes: CAPNS2 antibody at 8ug/ml+Mouse heart tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 28 kDaObserved band size: 28 kDa)
CAPNS2, Polyclonal Antibody (Cat# AAA7050739)
Full Name
CAPNS2 Antibody
Reactivity
Human, Mouse
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysat,K562 whole cell lysateAll lanes: CAPN1 antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 82 KDaObserved band size: 82 KDa)
CAPN1, Polyclonal Antibody (Cat# AAA7051809)
Full Name
CAPN1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Human
Applications
EIA, WB, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blotAll lanes: CAPN6 antibody at 5ug/ml+Human placenta tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 75 kDaObserved band size: 75 kDa)
CAPN6, Polyclonal Antibody (Cat# AAA7004160)
Full Name
CAPN6 Antibody
Gene Names
CAPN6; CANPX; CAPNX; CalpM; DJ914P14.1
Reactivity
Human
Applications
EIA, WB, IHC
Purity
>95%,Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
SDS-Page
(Gel: 8%SDS-PAGELysate: 40 ΞOgLane 1-2: Jurkat and A549 cell lysatesPrimary antibody: CAPN14 Antibody at dilution 1/1000Secondary antibody: Goat anti rabbit IgG at 1/5000 dilutionExposure time: 5 minutes)
CAPN14, Polyclonal Antibody (Cat# AAA9429546)
Full Name
CAPN14 Antibody
Reactivity
Human
Applications
WB, IHC
Purity
Antigen affinity purification
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Activity Data
(Figure. The binding activity of CAPN3 with TTN.Calpain 3 is a calcium-dependent cysteine protease mainly expressed in skeletal muscle. In humans, calpain 3 is encoded by the CAPN3 gene. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. Besides, Titin (TTN) has been identified as an interactor of CAPN3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CAPN3 and recombinant human TTN. Briefly, CAPN3 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TTN-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CAPN3 and TTN was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 3 (CAPN3), Active Protein (Cat# AAA2105179)
Full Name
Active Calpain 3 (CAPN3)
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A; LGMDD4; LGMDR1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $485/0.05 mg | $755/0.1 mg | $935/0.2 mg | $1,820/0.5 mg | $2,695/1 mg | $6,585/5 mg
Western Blot (WB)
(Anti-Calpain 1 antibody, MBS175459, Western blottingLane 1: HT1080 Cell LysateLane 2: COLO320 Cell Lysate )
Calpain 1, Polyclonal Antibody (Cat# AAA175459)
Full Name
Anti-Calpain 1 antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody (MBS9231851). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
CAPN2, Monoclonal Antibody (Cat# AAA9231851)
Full Name
CAPN2 Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human, Rat
Predicted: Mouse
Predicted: Mouse
Applications
WB, IHC-P, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using CAPN1 Antibody(green) compared to an isotype control of mouse IgG1(blue). MBS9200385 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
CAPN1, Monoclonal Antibody (Cat# AAA9200385)
Full Name
CAPN1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Activity Data
(Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 1, Large Subunit (CAPN1), Active Protein (Cat# AAA2097301)
Full Name
Active Calpain 1, Large Subunit (CAPN1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$220/0.01 mg | $465/0.05 mg | $710/0.1 mg | $880/0.2 mg | $1,715/0.5 mg | $2,555/1 mg | $6,250/5 mg
Immunohistochemistry (IHC)
(MBS9606965 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CAPN1, Polyclonal Antibody (Cat# AAA9606965)
Full Name
CAPN1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells, using CAPN10 antibody.)
CAPN10, Polyclonal Antibody (Cat# AAA9605322)
Full Name
CAPN10 Antibody
Gene Names
CAPN10; CANP10; NIDDM1
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
SDS-Page
(Gel: 6%SDS-PAGE lysate: 40 ¦Œg, Lane: 231 cell lysate, Primary antibody: MBS9429547£¨CAPN15 Antibody) at dilution 1/450 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
CAPN15, Polyclonal Antibody (Cat# AAA9429547)
Full Name
CAPN15 Antibody
Gene Names
CAPN15; SOLH
Reactivity
Human
Applications
WB, IHC
Purity
Antigen affinity purification
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Serum; Lane2: Mouse Placenta Tissue.)
Calpain 1, Large Subunit (CAPN1), Polyclonal Antibody (Cat# AAA2002191)
Full Name
Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1)
Gene Names
Capn1; Capa1; Capa-1; mu-calpin
Reactivity
Mouse
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
$160/0.1 mL | $205/0.2 mL | $340/0.5 mL | $415/1 mL | $1,205/5x1 mL
Immunofluorescence (IF)
(MBS9605323 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CAPN11, Polyclonal Antibody (Cat# AAA9605323)
Full Name
CAPN11 Antibody
Gene Names
CAPN11; calpain11
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).)
Calpastatin, Monoclonal Antibody (Cat# AAA4381359)
Full Name
Calpastatin
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
EIA, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of CAPN3 using NIH-3T3 whole cell lysates)
CAPN3, Polyclonal Antibody (Cat# AAA9609637)
Full Name
CAPN3 Antibody
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A; LGMDD4; LGMDR1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry - Paraffin (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
CAPN2 / Calpain 2 / M-Calpain, Monoclonal Antibody (Cat# AAA249312)
Full Name
Anti-CAPN2 / Calpain 2 / M-Calpain Antibody (clone 1F4) IHC-plus
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Mouse, Rat, Human, Monkey
Applications
IHC, WB, FC/FACS
Purity
Immunoaffinity purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Western Blot (WB)
(Western blot analysis of CAPNS2 using 293 whole cell lysates)
CAPNS2, Polyclonal Antibody (Cat# AAA9609635)
Full Name
CAPNS2 Antibody
Gene Names
CAPNS2; CSS2
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CAPN9 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
CAPN9, Polyclonal Antibody (Cat# AAA9133802)
Full Name
CAPN9 Polyclonal Antibody
Gene Names
CAPN9; GC36; nCL-4
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CAPN3 Antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
CAPN3, Polyclonal Antibody (Cat# AAA9131917)
Full Name
CAPN3 Polyclonal Antibody
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of CAPN2 using RAW264.7 whole lysates)
CAPN2, Polyclonal Antibody (Cat# AAA9608370)
Full Name
CAPN2 Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 1, Large Subunit (CAPN1), Active Protein (Cat# AAA2097302)
Full Name
Active Calpain 1, Large Subunit (CAPN1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$220/0.01 mg | $465/0.05 mg | $710/0.1 mg | $880/0.2 mg | $1,715/0.5 mg | $2,555/1 mg | $6,250/5 mg
Western Blot (WB)
(Western Blot analysis of 293T cell using CAPN1 Polyclonal Antibody at dilution of 1:450)
CAPN1, Polyclonal Antibody (Cat# AAA2517559)
Full Name
CAPN1 Polyclonal Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CAPN3 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
CAPN3, Polyclonal Antibody (Cat# AAA127766)
Full Name
CAPN3
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunofluorescence (IF)
(MBS9608410 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CAPNS1, Polyclonal Antibody (Cat# AAA9608410)
Full Name
CAPNS1 Antibody
Gene Names
CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of CAPN6 using NIH-3T3 whole cell lysates)
CAPN6, Polyclonal Antibody (Cat# AAA9609638)
Full Name
CAPN6 Antibody
Gene Names
CAPN6; CANPX; CAPNX; CalpM; DJ914P14.1
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human Blood Cells; Lane3: Human A431 Cells; Lane4: Mouse Spleen Tissue.)
Calpain, Small Subunit 1, Polyclonal Antibody (Cat# AAA2032788)
Full Name
Calpain, Small Subunit 1 (CAPNS1) Polyclonal Antibody
Gene Names
CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL
Western Blot (WB)
(Western blot analysis of CAPN7 using HepG2 whole cell lysates)
CAPN7, Polyclonal Antibody (Cat# AAA9609639)
Full Name
CAPN7 Antibody
Gene Names
CAPN7; PALBH; CALPAIN7
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Calpastatin using anti-Calpastatin antibody (MBS178604). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpastatin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Calpastatin at approximately 100KD. The expected band size for Calpastatin is at 76KD. )
Calpastatin, Polyclonal Antibody (Cat# AAA178604)
Full Name
Anti-Calpastatin Antibody
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Rabbit Anti-CAPN1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human heart TissueObserved Staining: CytoplasmicPrimary Antibody Concentration: N/AOther Working Concentrations: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
CAPN1, Polyclonal Antibody (Cat# AAA3215016)
Full Name
CAPN1 antibody - middle region
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Cow, Goat, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish
Applications
WB, IHC
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CAPN13 using Jurkat whole cell lysates)
CAPN13, Polyclonal Antibody (Cat# AAA9609636)
Full Name
CAPN13 Antibody
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of Lovo cell using CAST Polyclonal Antibody at dilution of 1:750)
CAST, Antibody (Cat# AAA2516852)
Full Name
CAST Polyclonal Antibody
Gene Names
CAST; BS-17
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Immunofluorescence (IF)
(MBS9605324 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CAPN12, Polyclonal Antibody (Cat# AAA9605324)
Full Name
CAPN12 Antibody
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of H460 cells, using CAST antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
CAST, Polyclonal Antibody (Cat# AAA125016)
Full Name
CAST Polyclonal Antibody
Gene Names
CAST; BS-17
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CAPNS1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
CAPNS1, Polyclonal Antibody (Cat# AAA9125779)
Full Name
CAPNS1 Polyclonal Antibody
Gene Names
CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CAST antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
CAST, Polyclonal Antibody (Cat# AAA9128762)
Full Name
CAST Polyclonal Antibody
Gene Names
CAST; BS-17; PLACK
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$210/0.02 mL | $265/0.05 mL | $380/0.1 mL | $540/0.2 mL | $2,285/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CAPN10 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
CAPN10, Polyclonal Antibody (Cat# AAA9129741)
Full Name
CAPN10 Polyclonal Antibody
Gene Names
CAPN10; CANP10; NIDDM1
Reactivity
Human
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of Calpain 2 using anti-Calpain 2 antibody (MBS1750836).
Calpain 2 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calpain 2 Antibody (MBS1750836) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
)
Calpain 2, Polyclonal Antibody (Cat# AAA1750836)
Full Name
Anti-Calpain 2 Picoband Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of rat heart, using CAPN6 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
CAPN6, Polyclonal Antibody (Cat# AAA9133797)
Full Name
CAPN6 Polyclonal Antibody
Gene Names
CAPN6; CANPX; CAPNX; CalpM; DJ914P14.1
Reactivity
Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysates from MCF-7,A549,A431 cell line (from left to right), using CAPN1 Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
CAPN1, Monoclonal Antibody (Cat# AAA9231801)
Full Name
CAPN1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunofluorescence (IF)
(MBS9605325 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CAPN9, Polyclonal Antibody (Cat# AAA9605325)
Full Name
CAPN9 Antibody
Gene Names
CAPN9; GC36; nCL-4
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
SDS-Page
(SDS-PAGE Analysis Purified Spectrin beta III Mouse Monoclonal Antibody (RG/26). Confirmation of Purity and Integrity of Antibody.)
Spectrin beta I (SPTB), Monoclonal Antibody (Cat# AAA4381267)
Full Name
Spectrin beta I (SPTB)
Gene Names
SPTBN2; SCA5; SCAR14; GTRAP41
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
