Search results for "PARASITES"

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Testing Data (Published investigator image: The inhibition of the V-ATPase acquisition on phagosomes is specific for the promastigote stage.A -C, BMM cells were infected with either WT promastigotes or amastigotes for 2 h and 24 h, fixed and stained for V-ATPase (green), LPG (red) and DNA (blue) (A) or V-ATPase (green), LAMP-1 (red) and DNA (blue) (B). A and B, Confocal images illustrating V-ATPase acquisition on parasite-containing phagosomes (white arrowheads). C, V-ATPase acquisition was determined on at least 100 phagosomes for each condition and expressed as a percentage of recruitment. Three independent experiments were performed and the bars show the standard deviations of one representative triplicate (**, p=0.005; p values compare the acquisition of V-ATPase on phagosomes containing promastigotes vs amastigotes parasites). Bar, 3 um.From: Vinet AF, Fukuda M, Turco SJ, Descoteaux A (2009) The Leishmania donovani Lipophosphoglycan Excludes the Vesicular Proton-ATPase from Phagosomes by Impairing the Recruitment of Synaptotagmin V. PLoS Pathog 5(10): e1000628.)

Testing Data (Published investigator image: Recruitment of Syt V to GM1-containing microdomains of phagosome membranes is prevented by LPG.A, BMM were allowed to internalize Zym for 30 min, fixed and stained for endogenous Syt V (green) and GM1 (red). White arrowheads indicate examples of colocalization between Syt V and GM1-positive microdomains, indicating a Syt V enrichment on these microdomains. B and C, BMM were either left untreated or treated with 10 mmol/L MbetaCD for 1 h before the internalization of Zym for 30 and 120 min. Cells were then fixed and stained for Syt V and LAMP-1. Representative confocal images of Syt V recruitment on cells with or without MbetaCD treatment is presented (B), white arrowheads indicate phagosomes. Syt V acquisition is expressed as a percentage of phagosome recruitment for Syt V. At least 100 phagosomes for each condition were assessed. Three independent experiments were performed and the bars show the standard deviations of one representative triplicate (C) (**, p=0.005). D, BMM were allowed to internalize Zym-LPG for 30 min, fixed and stained for LPG (green) and GM1 (red). White arrowheads indicate a colocalization between LPG and GM1-positive rafts. BMM were allowed to internalize Zym (E, upper panel) or LPG-Zym (E, lower panel) for 30 min, fixed and stained for Syt V (blue), LPG (green) and GM1 (red). Blue arrowheads indicate a local Syt V acquisition on phagosome membrane and yellow arrowheads indicate a local colocalization between GM1 microdomains and LPG. A rim around each phagosome was manually traced with a one pixel width and fluorescence intensity profile of Syt V in blue, LPG in green and GM1 in red were represented in a graph (F). Bars, 3 um (A, B and D) or 1 um (E).From: Vinet AF, Fukuda M, Turco SJ, Descoteaux A (2009) The Leishmania donovani Lipophosphoglycan Excludes the Vesicular Proton-ATPase from Phagosomes by Impairing the Recruitment of Synaptotagmin V. PLoS Pathog 5(10): e1000628.)

Testing Data (Published investigator image: CsA-treated L. donovani promastigotes show altered morphology. Promastigotes were incubated with 0.15% ethanol or 15 uM (B, C) or 20 uM (A) CsA at 26 degree C, pH 7.4 for 72 hours. Axenic amastigotes were prepared as described in experimental procedure. 107 cells were fixed with either methanol for Giemsa staining (A), or 2.5% glutaraldehyde for scanning electron microscopy (B). The bar corresponds to 1 um (B) and 5 um (A). Two independent experiments were performed and representative fields are shown. (C) Flagellum length measurement. CsA-treated and solvent treated cells L. donovani promastigotes were fixed in methanol and stained with anti-tubulin monoclonal antibody. Flagellum length was measured from a total of 180 cells each for control and CsA-treated samples. Only cells with a single flagellum that was completely visible and fully in focus were taken into account. Samples were observed with a DMR Leica microscope and images were captured with a Cool Snap HQ camera (Roper Scientific). Images were analysed using the IPLab Spectrum 3.9 software (Scanalytics & BD Biosciences) and flagellum length was measured using ImageJ (NIH). (D) Immunoblot analysis of CsA treated parasites. Parasites were treated with solvent or 15 uM CsA for 72 hours, lysed in 1x Laemmli buffer, and lysates equal to 2x107 cells were analyzed by immunoblotting. Promastigote specific marker LPG (upper), amastigote specific marker A2 (middle) and a-tubulin (lower) were analyzed. Two independent experiments which gave identical results were performed.From: Yau W-L, Blisnick T, Taly J-F, Helmer-Citterich M, Schiene-Fischer C, et al. (2010) Cyclosporin A Treatment of Leishmania donovani Reveals Stage-Specific Functions of Cyclophilins in Parasite Proliferation and Viability. PLoS Negl Trop Dis 4(6): e729.)

LEISHMANIA LPG, Monoclonal Antibody (Cat# AAA210110)

Full Name

MOUSE ANTI LEISHMANIA LPG (REPEAT EPITOPE)

Applications

EIA, IB, IF

Pricing

$935/0.5 mL | $4,065/5x0.5 mL

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Protein concentration determined espectrophotometrically (This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient Therefore, we have measured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 μl of the protein were analysed.)

K39, Recombinant Protein (Cat# AAA505052)

Full Name

K39 recombinant

Applications

EIA, WB, DB, LF

Pricing

$335/0.1 mg | $945/1 mg | $1,755/2 mg | $3,760/5 mg

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Immunofluorescence (IF) (Immunofluorescent analysis of GFP using either natural fluorescence (green) or an GFP antibody (red) in Hela(human cervical epithelial adenocarcinoma cell line) cells transfected with GFP recombinant protein. Formalin-fixed cells were permeabilized with 0. 1% Triton X-100 for 10 minutes at room temperature and blocked with 3% BSA for 30 minutes at room temperature. Cells were probed with an GFP monoclonal antibody (Product # MBS9200313) at a dilution of 1:25 for 1 hour at 37°C, and incubated with DyLight 555 goat anti-mouse IgG secondary antibody (Product # please inquire) at a dilution of 1:200 for 60 minutes at 37°C. The nuclear counter stain is DAPI (blue).)

GFP Tag, Monoclonal Antibody (Cat# AAA9200313)

Full Name

GFP Tag Antibody

Reactivity

Human

Applications

IF, EIA, WB

Purity

This antibody is purified through a protein G column, followed by dialysis against PBS.

Pricing

$190/0.05 mL | $435/0.2 mL | $1,705/5x0.2 mL

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Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD209 Monoclonal Antibody (C209/1781).)

CD209/DC-SIGN, Monoclonal Antibody (Cat# AAA4380016)

Full Name

CD209/DC-SIGN (Pathogen Receptor on Dendritic Cells)

Gene Names

CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1

Reactivity

Human. Others not known.

Applications

FC/FACS, IF, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Testing Data (TD) (Figure 1. SDS-PAGE analysis (12.%) of 3 ul of recombinant ChimChagas 2. Purity is > 98% as determined by gel electrophoresis.Bands which are slightly smaller correspond to this same protein as it is showed in a western blot performed with a his-tag monoclonal antibody)

ChimChagas2, Recombinant Protein (Cat# AAA505072)

Full Name

Recombinant chimeric antigen ChimChagas2 for Trypanosoma cruzi

Purity

>98% in a SDS-PAGE

Pricing

$900/1 mg | $3,755/5 mg

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CD169, Monoclonal Antibody (Cat# AAA225720)

Full Name

Rat Anti Mouse CD169: RPE

Applications

FC/FACS

Pricing

$500/100 Tests | $2,090/5x100 Tests

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Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD209 Mouse Monoclonal Antibody (rC209/1781).)

CD209/DC-SIGN, Antibody (Cat# AAA4380977)

Full Name

CD209/DC-SIGN (Pathogen Receptor on Dendritic Cells)

Gene Names

CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1

Reactivity

Human

Applications

IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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KMP-11, Recombinant Protein (Cat# AAA505006)

Full Name

KMP-11 recombinant

Applications

EIA, WB, LF

Pricing

$335/0.1 mg | $945/1 mg | $1,755/2 mg | $3,760/5 mg

IgE, Monoclonal Recombinant Antibody (Cat# AAA488431)

Full Name

Anti-IgE [TES-C21]

Reactivity

Human

Applications

BL, EIA

Purity

Protein A Affinity Purified

Pricing

$495/0.2 mg | $2,025/5x0.2 mg

IgE, Monoclonal Recombinant Antibody (Cat# AAA488430)

Full Name

Anti-IgE [TES-C21]

Reactivity

Human

Applications

BL, EIA

Purity

Protein A Affinity Purified

Pricing

$495/0.2 mg | $2,025/5x0.2 mg

CD209/DC-SIGN, Monoclonal Antibody (Cat# AAA4380585)

Full Name

CD209/DC-SIGN (Pathogen Receptor on Dendritic Cells)

Gene Names

CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1

Reactivity

Human. Others not known.

Applications

Formalin-Fixed

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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1F8, Recombinant Protein (Cat# AAA505020)

Full Name

1F8 recombinant

Applications

EIA, WB

Purity

0.98

Pricing

$335/0.1 mg | $945/1 mg | $1,755/2 mg | $3,760/5 mg

IFN beta, Active Protein (Cat# AAA538114)

Full Name

IFN beta protein (Mouse)

Gene Names

Ifnb1; Ifb; IFNB; IFN-beta

Applications

User optimized

Purity

> 95% pure

Pricing

$995/100K Units | $4,290/5x100K Units

KINETOPLASTID MEMBRANE PROTEIN 11, Monoclonal Antibody (Cat# AAA210111)

Full Name

MOUSE ANTI KINETOPLASTID MEMBRANE PROTEIN 11

Applications

EIA, IB, IF

Pricing

$955/0.5 mL | $4,140/5x0.5 mL

LEISHMANIA MAJOR SURFACE PROTEASE gp63, Monoclonal Antibody (Cat# AAA210112)

Full Name

MOUSE ANTI LEISHMANIA MAJOR SURFACE PROTEASE gp63

Applications

EIA, IF

Pricing

Full Name

IFNAR2 antibody (PE)

Gene Names

IFNAR2; IFN-R; IFNBR; IFN-R-2

Reactivity

Full Name

Chlamydia antibody

Applications

IF

Pricing

$210/1 mg | $935/5x1 mg

Leishmania, Major Surface Protease gp63, Monoclonal Antibody (Cat# AAA609779)

Full Name

Leishmania, Major Surface Protease gp63 (Cell Surface Protease, Leishmanolysin, Major Surface Glycoprotein, Promastigote Surface Endopeptidase, Protein gp63)

Applications

EL/EIA, IF

Purity

Ascites
Ascites

Pricing

$1,170/0.5 mL | $5,250/5x0.5 mL

LDH, Monoclonal Antibody (Cat# AAA833413)

Full Name

LDH Pan antibody

Applications

EIA

Purity

> 95% pure

Pricing

$305/1 mg | $1,370/5x1 mg

IFN beta, Monoclonal Antibody (Cat# AAA530525)

Full Name

IFN beta antibody

Gene Names

IFNB1; IFB; IFF; IFNB

Reactivity

Human

Applications

EIA, IHC, IP, WB

Pricing

$505/0.05 mg | $2,080/5x0.05 mg

Chlamydia, Monoclonal Antibody (Cat# AAA532756)

Full Name

Chlamydia antibody

Applications

EIA, IF, IHC

Purity

Chlamydia antibody was purified by Protein A affinity chromatography

Pricing

$1,730/0.5 mg | $7,765/5x0.5 mg

CD234, Polyclonal Antibody (Cat# AAA224136)

Full Name

RABBIT ANTI HUMAN CD234 (N-TERMINAL)

Gene Names

ACKR1; FY; Dfy; GPD; DARC; GpFy; CCBP1; CD234; WBCQ1

Applications

WB

Pricing

Full Name

Malaria Pan mAb2-coating

Applications

EIA

Purity

Purification: lon-exchange chromatography purified
Purity: >95%

Pricing

$265/1 mg | $830/5 mg | $1,550/10 mg | $2,420/20 mg | $5,815/50 mg

IL13, Polyclonal Antibody (Cat# AAA535943)

Full Name

IL13 antibody

Gene Names

IL13; P600; IL-13

Reactivity

Murine IL-13

Applications

EIA, WB

Pricing

$425/0.05 mg | $1,905/5x0.05 mg

IFNAR2, Monoclonal Antibody (Cat# AAA531050)

Full Name

IFNAR2 antibody

Gene Names

IFNAR2; IFN-R; IFNABR; IFNARB; IFN-alpha-REC

Reactivity

Human

Applications

FC/FACS

Pricing

$1,275/0.05 mg | $5,725/5x0.05 mg

Chlamydia, Monoclonal Antibody (Cat# AAA830551)

Full Name

Chlamydia antibody

Applications

IF

Purity

Chlamydia antibody was purified by Protein A affinity chromatography

Pricing

$380/0.05 mg | $1,700/5x0.05 mg

CD234, Polyclonal Antibody (Cat# AAA223703)

Full Name

RABBIT ANTI HUMAN CD234 (C-TERMINAL)

Gene Names

ACKR1; FY; Dfy; GPD; DARC; GpFy; CCBP1; CD234; WBCQ1

Reactivity

Reacts with: Mouse, Rat
N.B. Antibody reactivity and working conditions may vary between species.

Applications

WB

Pricing

$420/0.1 mg | $1,735/5x0.1 mg

Malaria LDH, Monoclonal Antibody (Cat# AAA838698)

Full Name

Malaria LDH antibody

Applications

EIA

Pricing

$615/0.5 mg | $2,770/5x0.5 mg

IFN beta, Monoclonal Antibody (Cat# AAA532112)

Full Name

IFN beta antibody

Gene Names

IFNB1; IFB; IFF; IFNB

Reactivity

Human

Applications

Full Name

CD23 antibody

Gene Names

Fcer2; CD23; Fcer2a

Reactivity

Murine

Applications

FC/FACS, IP

Pricing

$520/0.5 mg | $2,330/5x0.5 mg

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120 results for "PARASITES" - showing 1-50

LEISHMANIA LPG, Monoclonal Antibody (Cat# AAA210110)

K39, Recombinant Protein (Cat# AAA505052)

GFP Tag, Monoclonal Antibody (Cat# AAA9200313)

CD209/DC-SIGN, Monoclonal Antibody (Cat# AAA4380016)

ChimChagas2, Recombinant Protein (Cat# AAA505072)

CD169, Monoclonal Antibody (Cat# AAA225720)

CD209/DC-SIGN, Antibody (Cat# AAA4380977)

KMP-11, Recombinant Protein (Cat# AAA505006)

IgE, Monoclonal Recombinant Antibody (Cat# AAA488431)

IgE, Monoclonal Recombinant Antibody (Cat# AAA488430)

CD209/DC-SIGN, Monoclonal Antibody (Cat# AAA4380585)

1F8, Recombinant Protein (Cat# AAA505020)

IFN beta, Active Protein (Cat# AAA538114)

KINETOPLASTID MEMBRANE PROTEIN 11, Monoclonal Antibody (Cat# AAA210111)

LEISHMANIA MAJOR SURFACE PROTEASE gp63, Monoclonal Antibody (Cat# AAA210112)

IFNGR2, Monoclonal Antibody (Cat# AAA532362)

IgE, Monoclonal Native Protein (Cat# AAA230324)

IFN alpha 2, Active Protein (Cat# AAA537874)

IFN alpha, Polyclonal Antibody (Cat# AAA534576)

LDH, Monoclonal Antibody (Cat# AAA833089)

IFNGR2, Monoclonal Antibody (Cat# AAA531814)

IFNAR2, Monoclonal Antibody (Cat# AAA530109)

IFNAR2, Monoclonal Antibody (Cat# AAA532467)

Goat anti Human IgE, Secondary Antibody (Cat# AAA534551)

CD23, Monoclonal Antibody (Cat# AAA531956)

IFN gamma, Monoclonal Antibody (Cat# AAA531114)

Goat anti Canine IgE (HRP), Secondary Antibody (Cat# AAA539036)

Goat anti Canine IgE, Secondary Antibody (Cat# AAA535353)

Chlamydia, Monoclonal Antibody (Cat# AAA531285)

Leishmania, Major Surface Protease gp63, Monoclonal Antibody (Cat# AAA609779)

LDH, Monoclonal Antibody (Cat# AAA833413)

IFN beta, Monoclonal Antibody (Cat# AAA530525)

Chlamydia, Monoclonal Antibody (Cat# AAA532756)

CD234, Polyclonal Antibody (Cat# AAA224136)

IFN beta 1a, Active Protein (Cat# AAA539593)

IL13, Polyclonal Antibody (Cat# AAA534461)

IFN beta, Monoclonal Antibody (Cat# AAA531408)

Malaria Pan LDH, Monoclonal Antibody (Cat# AAA478003)

IL13, Polyclonal Antibody (Cat# AAA535943)

IFNAR2, Monoclonal Antibody (Cat# AAA531050)

Chlamydia, Monoclonal Antibody (Cat# AAA830551)

CD234, Polyclonal Antibody (Cat# AAA223703)

Malaria LDH, Monoclonal Antibody (Cat# AAA838698)

IFN beta, Monoclonal Antibody (Cat# AAA532112)

Chlamydia, Monoclonal Antibody (Cat# AAA831975)

CD23, Monoclonal Antibody (Cat# AAA532267)

IFN gamma, Monoclonal Antibody (Cat# AAA530807)

IFN gamma, Monoclonal Antibody (Cat# AAA530681)

IFN beta, Polyclonal Antibody (Cat# AAA534497)

CD23, Monoclonal Antibody (Cat# AAA532357)

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