Mouse anti-Human CD209/DC-SIGN Antibody | anti-CD209 antibody

CD209/DC-SIGN (Pathogen Receptor on Dendritic Cells)

Cat. Num. AAA4380977

• Gene Names: CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD209/DC-SIGN; CD209/DC-SIGN (Pathogen Receptor on Dendritic Cells); CD209; CDSIGN; CIRE; CLEC4L; DC-SIGN; DC-SIGN1; DCSIGN; Dendritic cell-specific ICAM-3 Grabbing Non-integrin 1; HIV GP120 Binding Protein; SIGN-R1; SIGNR5; anti-CD209 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1, kappa
• Clone Number: rC209/1781
• Specificity: DC-SIGN is a transmembrane receptor that is expressed on the surface of dendritic cells and macrophages. It is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization, which allows the receptor to bind multivalent ligands with high avidity.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 404

• Applicable Applications for anti-CD209 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: THP-1 or HeLa cells (IF/FACS). Human liver or spleen lysate (WB). Human small intestine (IHC).
• Immunogen: Recombinant human CD209 protein fragment (exact sequence is proprietary)
• Cellular Localization: Cell Surface and secreted
• Hu-chromosome Location: 19p13
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD209 Mouse Monoclonal Antibody (rC209/1781).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD209 Mouse Monoclonal Antibody (rC209/1781).)

SDS-Page

(SDS-PAGE Analysis Purified CD209 Mouse Monoclonal Antibody (rC209/1781). Confirmation of Purity and Integrity of Antibody.)

References

Steinman, R.M. 2000. DC-SIGN: A guide to some mysteries of Dendritic cells. Cell 100: 491-494.

NCBI and Uniprot Product Information

• NCBI GI #: 13383468
• NCBI GeneID: 30835
• UniProt Accession #: Q9NNX6
• Molecular Weight: 45kDa
• NCBI Official Full Name: DC-SIGN
• NCBI Official Synonym Full Names: CD209 molecule
• NCBI Official Symbol: CD209
• NCBI Official Synonym Symbols: CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
• NCBI Protein Information: CD209 antigen
• UniProt Protein Name: CD209 antigen
• Protein Family: CD209 antigen
• UniProt Gene Name: CD209
• UniProt Synonym Gene Names: CLEC4L; DC-SIGN; DC-SIGN1
• UniProt Entry Name: CD209_HUMAN

NCBI Description

This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.[provided by RefSeq, Feb 2009]

Uniprot Description

CD209: Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri- mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. Homotetramer. Binds to many viral surface glycoproteins such as HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus envelope glycoproteins, cytomegalovirus gB, HCV E2 and dengue virus major envelope protein E. Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: mannose binding; protein binding; peptide antigen binding; metal ion binding; virion binding; carbohydrate binding

Biological Process: cell-cell recognition; heterophilic cell adhesion; antigen processing and presentation; leukocyte adhesion; virus-host interaction; regulation of T cell proliferation; innate immune response; peptide antigen transport; endocytosis; viral genome replication; virion attachment to host cell surface receptor

Disease: Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To