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234 results for " Molecular processes" - showing 1-50
Sequence
Tubulin Beta (TUBb), Active Protein (Cat# AAA2097299)
Full Name
Active Tubulin Beta (TUBb)
Gene Names
TUBB; M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$240/0.01 mg | $520/0.05 mg | $810/0.1 mg | $1,000/0.2 mg | $1,955/0.5 mg | $2,895/1 mg | $7,080/5 mg
Activity Data
(JAK2 (Tyrosine-protein kinase JAK2) is a tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. JAK2 is considered to associate with some type I receptors, including EPOR (Erythropoietin receptor), therefore participates in cellular signal transduction. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK2 and recombinant human EPOR. Briefly, JAK2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL JAK2 were then transferred to EPOR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of JAK2 and EPOR was shown in Figure 1, and this effect was in a dose dependent manner.)
Janus Kinase 2 (JAK2), Active Protein (Cat# AAA2097244)
Full Name
Active Janus Kinase 2 (JAK2)
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$205/0.01 mg | $420/0.05 mg | $640/0.1 mg | $795/0.2 mg | $1,540/0.5 mg | $2,295/1 mg | $5,605/5 mg
Western Blot (WB)
(Western blot analysis of lysates from H-4-II-E, mouse NIH/3T3 cell line (from left to right), using HSPA8 Antibody (C-term). MBS9208423 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
HSPA8, Polyclonal Antibody (Cat# AAA9208423)
Full Name
HSPA8 Antibody (C-term)
Gene Names
HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
Reactivity
Human (Predicted Reactivity: Bovine, Hamster, Mouse, Rat)
Applications
IF, EIA, IHC, FC/FACS, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)
Rubicon, Polyclonal Antibody (Cat# AAA809904)
Full Name
Rubicon Antibody
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Activity Data
(Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1,is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Besides, Colony Stimulating Factor Receptor, Macrophage (M-CFS-R) has been identified as an interactor of M-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse M-CSF and recombinant mouse MCFSR Briefly, M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to M-CFS-R-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-M-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of M-CSF and M-CFS-R was shown in Figure 1, and this effect was in a dose dependent manner.)
Colony Stimulating Factor 1, Macrophage (MCSF), Active Protein (Cat# AAA2097263)
Full Name
Active Colony Stimulating Factor 1, Macrophage (MCSF)
Gene Names
Csf1; op; Csfm; MCSF; C87615
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$205/0.01 mg | $415/0.05 mg | $635/0.1 mg | $790/0.2 mg | $1,530/0.5 mg | $2,280/1 mg | $5,565/5 mg
Activity Data
(Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, rat RAGE shares similarities with human RAGE in amino acids sequence with the identity of 80.0%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein B (S100B), Active Protein (Cat# AAA2097246)
Full Name
Active S100 Calcium Binding Protein B (S100B)
Gene Names
S100b; S100P
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Activity Data
(Regenerating Islet Derived Protein 3 Beta (REG3b) also known as PAP-I and HIP is bactericidal C-type lectin which acts against several intestinal Gram-positive bacteria and Gram-negative bacteria. The Reg family proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. To test the effect of REG3b on cell proliferation of SK-N-SH, SK-N-SH cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of REG3b. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C .Cell proliferation of SK-N-SH cells after incubation with REG3b for 72h observed by inverted microscope was shown in Figure 1.)
Regenerating Islet Derived Protein 3 Beta (REG3b), Active Protein (Cat# AAA2097321)
Full Name
Active Regenerating Islet Derived Protein 3 Beta (REG3b)
Gene Names
Reg3b; HIP; Pap; PAP1; REG-III
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$235/0.01 mg | $510/0.05 mg | $790/0.1 mg | $980/0.2 mg | $1,915/0.5 mg | $2,830/1 mg | $6,925/5 mg
Immunohistochemistry (IHC)
(Anti-INA / Alpha Internexin antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)
INA / Alpha Internexin, Polyclonal Antibody (Cat# AAA248063)
Full Name
Chicken Polyclonal (IgY) to Human INA / Alpha Internexin
Gene Names
INA; NEF5; NF-66; TXBP-1
Reactivity
Human
Applications
IHC - Paraffin, ICC, IF, WB
Purity
Purified
Pricing
$610/0.05 mL | $2,685/5x0.05 mL
Western Blot (WB)
(FUS Antibody (C-term) (MBS9201324) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the FUS antibody detected FUS protein (arrow).)
FUS, Polyclonal Antibody (Cat# AAA9201324)
Full Name
FUS Antibody (C-term)
Gene Names
FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
Reactivity
Human, Mouse
Predicted: Bovine
Predicted: Bovine
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Sacsin using anti-Sacsin antibody (MBS1750427).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates,Lane 3: human Hela cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sacsin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Sacsin at approximately 521KD. The expected band size for Sacsin is at 521KD. )
Sacsin, Polyclonal Antibody (Cat# AAA1750427)
Full Name
Anti-Sacsin Picoband antibody
Gene Names
SACS; SPAX6; ARSACS; DNAJC29; PPP1R138
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Activity Data
(Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, mouse RAGE shares similarities with human RAGE in amino acids sequence with the identity of 78.2%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein B (S100B), Active Protein (Cat# AAA2097249)
Full Name
Active S100 Calcium Binding Protein B (S100B)
Gene Names
S100b; Bpb; AI850290
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Chemical Structure
(Chemical structure and design of FCR1 (SIH-180), showing FRET processes in oxidised form. Inset: photographs of cuvettes of FCR1 in oxidised and reduced forms under 365 nm excitation. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.)
S100 Calcium Binding Protein A8 (S100A8), Active Protein (Cat# AAA2105151)
Full Name
Active S100 Calcium Binding Protein A8 (S100A8)
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$160/0.01 mg | $285/0.05 mg | $440/0.1 mg | $540/0.2 mg | $1,035/0.5 mg | $1,525/1 mg | $3,730/5 mg
Activity Data
(JAK3 (Tyrosine-protein kinase JAK3) is a non-receptor tyrosine kinase that involved in various processes such as cell growth, development, or differentiation. Besides, STAM2 has been identified as an interactor of JAK3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK3 and recombinant human STAM2. Briefly, JAK3 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAM2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of JAK3 and STAM2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Janus Kinase 3 (JAK3), Active Protein (Cat# AAA2097317)
Full Name
Active Janus Kinase 3 (JAK3)
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Activity Data
(Plasminogen (Plg) can be converted into active plasmin by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Plasmin can dissolve fibrin blood clots, act on many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion. Plasmint also can activates collagenases, weakens the walls of the Graafian follicle, cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Besides, Actin Beta (ACTb) has been identified as an interactor of Plg, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Plg and recombinant rat ACTb. Briefly, Plg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Plg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of Plg and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.)
Plasminogen (Plg), Active Protein (Cat# AAA2097291)
Full Name
Active Plasminogen (Plg)
Gene Names
Plg; Ab1-346
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Activity Data
(Leptin is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Many of leptin's effects are mediated through neuropeptide- containing neurons and neuropeptide receptors in the hypothalamus. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Besides, Hexokinase 3, White Cell (HK3) has been identified as an interactor of LEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human LEP and recombinant human HK3. Briefly, LEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HK3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-LEPpAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of LEP and HK3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Leptin (LEP), Active Protein (Cat# AAA2097261)
Full Name
Active Leptin (LEP)
Gene Names
LEP; OB; OBS; LEPD
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$130/0.01 mg | $225/0.05 mg | $315/0.1 mg | $390/0.2 mg | $735/0.5 mg | $1,080/1 mg | $2,625/5 mg | $11,805/5x5 mg
Western Blot (WB)
(Staining (0.3ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
CMG1/CCDC2/IFT74, Polyclonal Antibody (Cat# AAA421519)
Full Name
Goat anti-CMG1/CCDC2/IFT74 Antibody
Gene Names
IFT74; CMG1; CCDC2; CMG-1
Reactivity
Tested: Human, Rat; Expected from sequence similarity: Human, Rat
Applications
EIA, WB, IHC, IF
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)
Rubicon, Polyclonal Antibody (Cat# AAA809917)
Full Name
Rubicon Antibody: HRP
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Activity Data
(FGF13 (Fibroblast growth factor 13) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant rat FGF13 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF13. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with FGF13 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF13 for 48h. The result was shown in Figure 2. It was obvious that FGF13 significantly increased cell viability of 3T3 cells.)
Fibroblast Growth Factor 13 (FGF13), Active Protein (Cat# AAA2097308)
Full Name
Active Fibroblast Growth Factor 13 (FGF13)
Gene Names
Fgf13; FGF-13
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$265/0.01 mg | $595/0.05 mg | $920/0.1 mg | $1,135/0.2 mg | $2,240/0.5 mg | $3,315/1 mg | $8,120/5 mg
Activity Data
(FGF23 (Fibroblast growth factor 23) is a member of the fibroblast growth factor family, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF23 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF23. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with FGF23 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF23 for 48h. The result was shown in Figure 2. It was obvious that FGF23 significantly increased cell viability of 3T3 cells.)
Fibroblast Growth Factor 23 (FGF23), Active Protein (Cat# AAA2097280)
Full Name
Active Fibroblast Growth Factor 23 (FGF23)
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$165/0.01 mg | $310/0.05 mg | $480/0.1 mg | $585/0.2 mg | $1,120/0.5 mg | $1,660/1 mg | $4,075/5 mg
Activity Data
(FGF22 (Fibroblast growth factor 22) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF22 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF22. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with FGF22 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF22 for 48h. The result was shown in Figure 2. It was obvious that FGF22 significantly increased cell viability of 3T3 cells.)
Fibroblast Growth Factor 22 (FGF22), Active Protein (Cat# AAA2097307)
Full Name
Active Fibroblast Growth Factor 22 (FGF22)
Gene Names
Fgf22; FGF-22; 2210414E06Rik
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
HO-2, Polyclonal Antibody (Cat# AAA8001822)
Full Name
HO-2 Antibody: Alkaline Phosphatase
Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
WB
Purity
Protein A Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)
Rubicon, Polyclonal Antibody (Cat# AAA809905)
Full Name
Rubicon Antibody: ATTO 390
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000013)
Full Name
IRGM Antibody
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000014)
Full Name
IRGM Antibody: ATTO 390
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000029)
Full Name
IRGM Antibody: RPE
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)
Rubicon, Polyclonal Antibody (Cat# AAA809916)
Full Name
Rubicon Antibody: FITC
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
HO-2, Polyclonal Antibody (Cat# AAA8001815)
Full Name
HO-2 Antibody: ATTO 488
Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
WB
Purity
Protein A Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)
Rubicon, Polyclonal Antibody (Cat# AAA809908)
Full Name
Rubicon Antibody: ATTO 594
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
HO-2, Polyclonal Antibody (Cat# AAA8001828)
Full Name
HO-2 Antibody: PerCP
Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
WB
Purity
Protein A Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)
Rubicon, Polyclonal Antibody (Cat# AAA809915)
Full Name
Rubicon Antibody: Biotin
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)
Rubicon, Polyclonal Antibody (Cat# AAA809919)
Full Name
Rubicon Antibody: PerCP
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000017)
Full Name
IRGM Antibody: ATTO 594
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
HO-2, Polyclonal Antibody (Cat# AAA8001814)
Full Name
HO-2 Antibody: ATTO 390
Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
WB
Purity
Protein A Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Activity Data
(Caspase 1 (CASP1) cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. It also has lots of other function, such as defenses against pathogens, cleaves and activates sterol regulatory element binding proteins (SREBPs), promotes apoptosis. Besides, Interleukin 1 Alpha (IL1a) has been identified as an interactor of CASP1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CASP1 and recombinant rat IL1a. Briefly, CASP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CASP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CASP1 and IL1a was shown in Figure 1, and this effect was in a dose dependent manner.)
Caspase 1 (CASP1), Active Protein (Cat# AAA2097294)
Full Name
Active Caspase 1 (CASP1)
Gene Names
Casp1; Ice; Il1bc
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$255/0.01 mg | $575/0.05 mg | $895/0.1 mg | $1,100/0.2 mg | $2,170/0.5 mg | $3,210/1 mg | $7,865/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000026)
Full Name
IRGM Antibody: HRP
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000025)
Full Name
IRGM Antibody: FITC
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000024)
Full Name
IRGM Antibody: Biotin
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Bag1 using anti-Bag1 antibody (MBS1750768).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bag1 at approximately 39KD. The expected band size for Bag1 is at 39KD.)
Bag1, Polyclonal Antibody (Cat# AAA1750768)
Full Name
Anti-Bag1 Picoband Antibody
Gene Names
BAG1; HAP; BAG-1; RAP46
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
HO-2, Polyclonal Antibody (Cat# AAA8001829)
Full Name
HO-2 Antibody: RPE
Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
WB
Purity
Protein A Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
HO-2, Polyclonal Antibody (Cat# AAA8001824)
Full Name
HO-2 Antibody: Biotin
Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
WB
Purity
Protein A Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)
Rubicon, Polyclonal Antibody (Cat# AAA809920)
Full Name
Rubicon Antibody: RPE
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
HO-2, Polyclonal Antibody (Cat# AAA8001817)
Full Name
HO-2 Antibody: ATTO 594
Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
WB
Purity
Protein A Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
HO-2, Polyclonal Antibody (Cat# AAA8001826)
Full Name
HO-2 Antibody: HRP
Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
WB
Purity
Protein A Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)
Rubicon, Polyclonal Antibody (Cat# AAA809913)
Full Name
Rubicon Antibody: Alkaline Phosphatase
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)
Rubicon, Polyclonal Antibody (Cat# AAA809906)
Full Name
Rubicon Antibody: ATTO 488
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
HO-2, Polyclonal Antibody (Cat# AAA8001825)
Full Name
HO-2 Antibody: FITC
Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
WB
Purity
Protein A Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000028)
Full Name
IRGM Antibody: PerCP
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000022)
Full Name
IRGM Antibody: Alkaline Phosphatase
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
HO-2, Polyclonal Antibody (Cat# AAA8001813)
Full Name
HO-2 Antibody
Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
WB
Purity
Protein A Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
