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Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Rabbit anti-Mouse; Rat HO-2 Polyclonal Antibody | anti-HO-2 antibody

HO-2 Antibody: FITC

Gene Names
HMOX2; HO-2
Reactivity
Mouse; Rat
Applications
Western Blot
Purity
Protein A Purified
Synonyms
HO-2; Polyclonal Antibody; HO-2 Antibody: FITC; Rabbit Anti-Human HO-2 Polyclonal; Heme oxygenase 2 Antibody; Heme oxygenase (decycling) 2 Antibody; Heme oxygenase (decyclizing) 2 Antibody; HMOX 2 Antibody; HMOX2 Antibody; HMOX2_HUMAN Antibody; HO 2 Antibody; HO2 antibody; anti-HO-2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse; Rat
Clonality
Polyclonal
Specificity
Detects ~36kda.
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
316
Applicable Applications for anti-HO-2 antibody
Western Blot (WB)
Application Notes
WB: 1:500
Target
HO-2
Immunogen
Synthetic peptide from the full length of Human HO-2
Immunogen Species
Human
Conjugate
FITC
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Preparation and Storage
Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Western Blot (WB)

(Western blot analysis of Mouse, Rat Brain showing detection of ~36 kDa HO-2 protein using Rabbit Anti-HO-2 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: . Lane 3: Mouse Brain. Lane 4: Rat Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~36 kDa.)

Western Blot (WB) (Western blot analysis of Mouse, Rat Brain showing detection of ~36 kDa HO-2 protein using Rabbit Anti-HO-2 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: . Lane 3: Mouse Brain. Lane 4: Rat Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~36 kDa.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,837 Da
NCBI Official Full Name
heme oxygenase 2 isoform b
NCBI Official Synonym Full Names
heme oxygenase 2
NCBI Official Symbol
HMOX2
NCBI Official Synonym Symbols
HO-2
NCBI Protein Information
heme oxygenase 2
UniProt Protein Name
Heme oxygenase 2
UniProt Gene Name
HMOX2
UniProt Synonym Gene Names
HO2; HO-2

NCBI Description

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

Research Articles on HO-2

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Product Notes

The HO-2 hmox2 (Catalog #AAA8001825) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HO-2 Antibody: FITC reacts with Mouse; Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HO-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500. Researchers should empirically determine the suitability of the HO-2 hmox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HO-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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