FCR1 small molecule

FCR1

Cat. Num. AAA807938

• Synonyms: FCR1; FRET ratiometric fluorescence-based redox sensor; 7-(Diethylamino)-N-((1r; 4r)-4-(2-(10-ethyl-2; 4-dioxo-4; 10-dihydrobenzo[g]pteridin-3(2H)-yl)acetamido)cyclohexyl)-2-oxo-2H-chromene-3-carboxamide (FCR1); FCR1 small molecule

• Host: Synthetic
• Form/Format: Orange solid

• Solubility: Soluble in DMSO
• Molecular Formula: C34H37N7O6
• Preparation and Storage: Store at -20 degree C.

Chemical Structure

(Chemical structure and design of FCR1 (SIH-180), showing FRET processes in oxidised form. Inset: photographs of cuvettes of FCR1 in oxidised and reduced forms under 365 nm excitation. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.)

Immunofluorescence (IF)

(Fluorescence behavior of FCR1 (SIH-180) in the oxidized (green) and reduced (blue) forms, using 10 uM. Excitation: 405 nm. Reduced Emission: 475 nm. Oxidized Emission: 520 nm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.)

Testing Data

(Two photon - confocal microscopy imaging of HeLa cells treated with FCR1 (SIH-180, 10 uM, 15 min, ex = 820 nm) and (a) N-acetyl cysteine (50 uM, 30 min), (b) vehicle control and (c) H2O2 (50 uM, 30 min) in blue and green channels. The pseudo colour ratio images indicate the ratio of emission intensity in the green channel to blue channel. Scale bar represents 20 um. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.)

Immunofluorescence (IF)

(Fluorescence lifetimes of the donor (420 - 470 nm) in HeLa cells treated with FCR1 (SIH-180, 10 uM, ex = 820 nm) and N-acetyl cysteine, vehicle control and H2O2. Pseudo-colour images represent mean lifetime. Scale bar represents 50 um. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.)

Flow Cytometry (FC/FACS)

(Flow cytometric studies of HeLa cells treated with FCR1 (SIH-180, 10 uM, ex = 405 nm) showing the fluorescence ratio (560 / 450 nm) when treated with N-acetyl cysteine (blue), vehicle control (green) and H2O2 (red). Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.)

Related Product Information for FCR1 small molecule

FCR1 or flavin coumarin redox sensor 1, is a novel ratiometric fluorescent redox sensor utilized in fluorescence lifetime imaging microscopy and flow cytometry. In the oxidized form, excitation of FCR1 at 405nm results in a green fluorescence with max emissions at 525nm. Treatment with a mild reducing agent (including sodium cyanoborohydride, DTT, and glutathione) reduces the flavin thereby decreasing the green fluorescence intensity, and increasing the blue. Re-oxidation can be achieved using mild oxidizing agents. In general, the FCR1 probe can be used to observe changes in oxidative capacity without interference from background effects.

Product Categories/Family for FCR1 small molecule

Small Molecules; Redox Probe; Cancer; Oxidative Stress

References

1. Kaur A., Haghighatbin M.A., Hogan C.F., and New E.J. (2015) Chem. Commun. Epub.