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Immunohistochemistry (IHC) (Anti-INA / Alpha Internexin antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)

Chicken anti-Human INA / Alpha Internexin Polyclonal Antibody | anti-INA antibody

Chicken Polyclonal (IgY) to Human INA / Alpha Internexin

Gene Names
INA; NEF5; NF-66; TXBP-1
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Purified
Synonyms
INA / Alpha Internexin; Polyclonal Antibody; Chicken Polyclonal (IgY) to Human INA / Alpha Internexin; Anti-INA / Alpha Internexin Antibody IHC-plus; INA; 66 kDa neurofilament protein; Alpha Internexin; Alpha-internexin; Alpha-Inx; NEF5; Neurofilament 5; Neurofilament-66; NF-66; TXBP-1; Neurofilament 5 (66kD); Human INA; anti-INA antibody
Ordering
For Research Use Only!
Host
Chicken
Reactivity
Human
Clonality
Polyclonal
Isotype
IgY
Purity/Purification
Purified
Form/Format
Liquid
Sequence Length
499
Applicable Applications for anti-INA antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
ICC (1:1000), IF (1:1000), IHC-P (1:500), WB (1:10000)
Usage: The IgY preparation can be diluted to 1:1000 and higher for immunofluorescence staining and 1:10000 and higher for western blotting. On western blots use a brain or spinal cord homogenate and look for a major band at SDS-PAGE apparent molecular weigh...
Target Species
Human
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-INA / Alpha Internexin antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)

Immunohistochemistry (IHC) (Anti-INA / Alpha Internexin antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)

Immunocytochemistry (ICC)

(Mixed neuron glia cultures stain with INA antibody (red) and counterstained with rabbit polyclonal antibody to Coronin 1a, (green) which is an excellent marker of microglia and lymphocytes. The chicken internexin antibody is an excellent marker of neuronal processes in these cultures.)

Immunocytochemistry (ICC) (Mixed neuron glia cultures stain with INA antibody (red) and counterstained with rabbit polyclonal antibody to Coronin 1a, (green) which is an excellent marker of microglia and lymphocytes. The chicken internexin antibody is an excellent marker of neuronal processes in these cultures.)

Western Blot (WB)

(Western blot of extract of rat brain stem crude extract stained with INA antibody, showing a single strong clean band at ~66kDa.)

Western Blot (WB) (Western blot of extract of rat brain stem crude extract stained with INA antibody, showing a single strong clean band at ~66kDa.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,391 Da
NCBI Official Full Name
alpha-internexin
NCBI Official Synonym Full Names
internexin neuronal intermediate filament protein, alpha
NCBI Official Symbol
INA
NCBI Official Synonym Symbols
NEF5; NF-66; TXBP-1
NCBI Protein Information
alpha-internexin; alpha-Inx; neurofilament 5 (66kD); 66 kDa neurofilament protein; neurofilament-66, tax-binding protein
UniProt Protein Name
Alpha-internexin
Protein Family
UniProt Gene Name
INA
UniProt Synonym Gene Names
NEF5; Alpha-Inx; NF-66; Neurofilament-66
UniProt Entry Name
AINX_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009]

Uniprot Description

INA: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; extracellular space; intermediate filament cytoskeleton; nuclear membrane; neurofilament

Molecular Function: structural constituent of cytoskeleton

Biological Process: substantia nigra development; neurofilament cytoskeleton organization and biogenesis; cell differentiation

Research Articles on INA

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Product Notes

The INA ina (Catalog #AAA248063) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Chicken Polyclonal (IgY) to Human INA / Alpha Internexin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's INA / Alpha Internexin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). ICC (1:1000), IF (1:1000), IHC-P (1:500), WB (1:10000) Usage: The IgY preparation can be diluted to 1:1000 and higher for immunofluorescence staining and 1:10000 and higher for western blotting. On western blots use a brain or spinal cord homogenate and look for a major band at SDS-PAGE apparent molecular weigh.. Researchers should empirically determine the suitability of the INA ina for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INA / Alpha Internexin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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