Search results for " Reducing Agents"

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Structural Formula

SDS-PAGE ([ DESCRIPTION] Effective Size Range: 10kDa to 70kDa. Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa. Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use. [ STORAGE BUFFER] 62.5mM Tris-H3P04 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol. [APPLICATION] Accurate protein sizing on SDS-polyacrylamide gels and Western blots. [USAGE] 1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20°C have returned to solution. Do not boil! 2. Load the following volumes of the Marker on a SDS-polyacrylamide gel. [ LOADING VOLUMES] 3~5uL per well for mini gel; 7uL per well for large gel. Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels. [ QUALITY CONTROL] The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands. [IMPORTANT NOTES] 1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation. 2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%. 3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer. 4. Don't load too much protein. See recommended load volumes in the manual. 5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins. 6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer. 7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer. 8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding. [ SHELF LIFE] Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.)

Dehydroepiandrosterone (DHEA), Polyclonal Antibody (Cat# AAA2006064)

Full Name

Polyclonal Antibody to Dehydroepiandrosterone (DHEA)

Reactivity

General

Applications

ICC, IHC, EIA, WB

Purity

Affinity Chromatography

Pricing

$180/0.1 mL | $220/0.2 mL | $390/0.5 mL | $475/1 mL | $1,320/5x1 mL

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Cellular Glutathione Peroxidase, Assay Kit (Cat# AAA480417)

Full Name

Cellular Glutathione Peroxidase Assay Kit

Pricing

$735/1 Kit | $3,365/5x1 Kit

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VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody (Cat# AAA438785)

Full Name

VEGF (Vascular Endothelial Growth Factor) Mouse Monoclonal Antibody

Gene Names

VEGFA; VPF; VEGF; MVCD1

Reactivity

Human

Applications

IF, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody (Cat# AAA438814)

Full Name

VEGF (Vascular Endothelial Growth Factor) Mouse Monoclonal Antibody

Gene Names

VEGFA; VPF; VEGF; MVCD1

Reactivity

Human, Mouse, Rat, Rabbit, Dog. Others not known.

Applications

IF, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

Protein, Assay Kit (Cat# AAA9711521)

Full Name

Protein Quantification Kit (BCA Assay)

Pricing

$165/500 Tests | $270/5000 Tests | $210/2000 Tests | $1,220/5x5000 Tests

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CD81, Monoclonal Antibody (Cat# AAA212343)

Full Name

HAMSTER ANTI MOUSE CD81

Gene Names

Cd81; Tapa1; Tapa-1; Tspan28

Reactivity

Rat

Applications

IHC-Fr, EIA, FC, IP, WB

Pricing

$340/0.1 mg | $1,375/5x0.1 mg

NDRG1, cDNA Clone (Cat# AAA1274185)

Full Name

NDRG1 cDNA Clone

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Pricing

$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock

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CD81, Monoclonal Antibody (Cat# AAA212342)

Full Name

HAMSTER ANTI MOUSE CD81

Gene Names

Cd81; Tapa1; Tapa-1; Tspan28

Reactivity

Rat

Applications

EIA, FC/FACS, IP, WB

Pricing

$475/0.25 mg | $1,995/5x0.25 mg

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Immunofluorescence (IF) (Fluorescence behavior of NpFR1 (SIH-181) in the oxidized (black) and reduced (red) forms, using 50 uM. Excitation: 405 or 488 nm (not shown). Emission: 490 - 600 nm, with peak at 545 nm.)

NpFR1, Small Molecule (Cat# AAA807939)

Full Name

NpFR1

Pricing

$575/1 mg | $2,350/5x1 mg

N-Myc Downstream Regulated 1, Active Protein (Cat# AAA143793)

Full Name

Recombinant Human N-Myc Downstream Regulated 1

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Purity

Greater than 95.0% as determined by SDS-PAGE.

Pricing

$180/0.01 mg | $250/0.05 mg | $1,925/1 mg | $8,435/5x1 mg

VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody (Cat# AAA4381331)

Full Name

VEGF (Vascular Endothelial Growth Factor)

Gene Names

VEGFA; VPF; VEGF; MVCD1

Reactivity

Human

Applications

EIA, IF

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

NDRG1-T346, Polyclonal Antibody (Cat# AAA9135645)

Full Name

Phospho-NDRG1-T346 pAb

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse

Applications

WB

Purity

Affinity Purification

Pricing

$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL

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CD81, Monoclonal Antibody (Cat# AAA215271)

Full Name

HAMSTER ANTI MOUSE CD81

Gene Names

Cd81; Tapa1; Tapa-1; Tspan28

Applications

EIA, FC/FACS, IP, WB

Pricing

$195/0.025 mg | $730/5x0.025 mg

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Western Blot (WB) (Western blot analysis of Hela whole cell lysates, using NDRG1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

NDRG1, Polyclonal Antibody (Cat# AAA9607518)

Full Name

NDRG1 Antibody

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, EIA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

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Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, 293T whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate, Mouse kidney tissueAll lanes: NDRG1 antibody at 0.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43, 36, 34 KDaObserved band size: 43 KDa)

NDRG1, Monoclonal Recombinant Antibody (Cat# AAA7114934)

Full Name

NDRG1 Antibody

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse

Applications

EIA, WB, IHC, IF

Purity

Affinity-Chromatography

Pricing

$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL

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NDRG1, Polyclonal Antibody (Cat# AAA127039)

Full Name

NDRG1 Polyclonal Antibody

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL

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Immunohistochemistry (IHC) (Anti-NDRG1 antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 0.7 ug/ml.)

NDRG1, Monoclonal Antibody (Cat# AAA2400638)

Full Name

Anti-NDRG1 Antibody (clone 2D7)

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified from ascites by Protein A

Pricing

$650/0.05 mg | $2,875/5x0.05 mg

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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NDRG1 antibody.)

NDRG1, Polyclonal Antibody (Cat# AAA2519964)

Full Name

NDRG1 Antibody

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Antigen affinity purification

Pricing

$405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL

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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NDRG1 antibody.)

NDRG1, Polyclonal Antibody (Cat# AAA9401419)

Full Name

NDRG1 Antibody

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse

Applications

WB, IHC, IF

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$300/0.05 mL | $390/0.1 mL | $1,610/5x0.1 mL

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Immunohistochemistry - Paraffin (IHC) (Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

NDRG1, Polyclonal Antibody (Cat# AAA249784)

Full Name

Anti-NDRG1 Antibody IHC-plus

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Mouse, Rat, Human

Applications

IHC, IF, WB

Purity

Affinity purified

Pricing

$610/0.05 mL | $2,685/5x0.05 mL

NDRG1-S330, Polyclonal Antibody (Cat# AAA9135660)

Full Name

Phospho-NDRG1-S330 pAb

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse

Applications

WB

Purity

Affinity Purification

Pricing

$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL

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VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody (Cat# AAA4381330)

Full Name

VEGF (Vascular Endothelial Growth Factor)

Gene Names

VEGFA; VPF; VEGF; MVCD1

Reactivity

Human

Applications

EIA, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Western Blot (WB) (Western Blot analysis of Human lymphoma tissue using NDRG1 Polyclonal Antibody at dilution of 1:1000)

NDRG1, Polyclonal Antibody (Cat# AAA2529197)

Full Name

NDRG1 Polyclonal Antibody

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse, Rat

Applications

EIA, WB, IHC

Purity

Antigen affinity purification

Pricing

$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL

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Western Blot (WB) (Western blot analysis of NDRG1 expression in Hela (A), SW480 (B), mouse kidney (C), mouse brain (D) whole cell lysates.)

NDRG1, Polyclonal Antibody (Cat# AAA8247520)

Full Name

Anti-NDRG1 Antibody

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$200/0.05 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL

NDRG1, Active Protein (Cat# AAA203387)

Full Name

NDRG1, 1-394aa, Human, His tag, E Coli (Bioactivity Validated)

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

$190/0.02 mg | $280/0.1 mg | $570/0.5 mg | $2,325/5x0.5 mg

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Western Blot (WB) (Western blot analysis of NDRG1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). NDRG1 (arrow) was detected using the purified Pab.)

NDRG1, Polyclonal Antibody (Cat# AAA9206039)

Full Name

NDRG1 Antibody (N-term)

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)

Applications

WB, EIA, IHC, FC/FACS

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL

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NDRG1, Polyclonal Antibody (Cat# AAA420361)

Full Name

Goat anti-NDRG1 Antibody

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

Applications

EIA, WB, IHC

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$335/0.1 mg

H1 (A/NewCaledonia/20/99)(H1N1), Monoclonal Antibody (Cat# AAA430026)

Full Name

Anti-H1 (A/NewCaledonia/20/99)(H1N1), clone 4

Applications

EIA, WB

Purity

Chromatography on protein A Sepharose

Pricing

$325/0.1 mg | $1,305/5x0.1 mg

HA (B/Tokyo/53/99), Monoclonal Antibody (Cat# AAA430056)

Full Name

Anti-HA (B/Tokyo/53/99) monoclonal antibody, clone 190

Applications

EIA, WB

Purity

Chromatography on protein A Sepharose

Pricing

$325/0.1 mg | $1,305/5x0.1 mg

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SDS-PAGE (SDS-PAGE analysis (12%) of 1 ul of recombinant LipL32. Purity is >95% as determined by gel electrophoresis.)

lipl32, Recombinant Protein (Cat# AAA505042)

Full Name

lipl32 recombinant

Applications

EIA

Pricing

$335/0.1 mg | $945/1 mg | $1,755/2 mg | $3,760/5 mg

Phosphodiesterase 1, Enzyme (Cat# AAA653995)

Full Name

Phosphodiesterase 1

Purity

Molecular Biology Grade

Pricing

$400/100 Units | $1,790/5x100 Units

Nucleoprotein, Monoclonal Antibody (Cat# AAA430051)

Full Name

Anti-Nucleoprotein (Influenza A/New Caledonia/20/99/H1N1), clone 108

Applications

EIA, WB, IHC

Purity

Protein A Sepharose chromatography

Pricing

$325/0.1 mg | $1,305/5x0.1 mg

Rubella (E1), Monoclonal Antibody (Cat# AAA312834)

Full Name

MAb to Rubella (E1)

Applications

Western Blot

Purity

Protein A chromatography

Pricing

$395/1 mg | $1,640/5x1 mg

H3 (A/Shangdong/9/93) (H3N2), Monoclonal Antibody (Cat# AAA430034)

Full Name

Anti-H3 (A/Shangdong/9/93) (H3N2), clone 246

Applications

EIA, WB

Purity

Chromatography on protein A Sepharose

Pricing

$325/0.1 mg | $1,305/5x0.1 mg

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Western Blot (Sample: Recombinant Osteonectin, Human)

SDS-PAGE ([ DESCRIPTION] Effective Size Range: 10kDa to 70kDa. Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa. Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use. [ STORAGE BUFFER] 62.5mM Tris-H3P04 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol. [APPLICATION] Accurate protein sizing on SDS-polyacrylamide gels and Western blots. [USAGE] 1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20°C have returned to solution. Do not boil! 2. Load the following volumes of the Marker on a SDS-polyacrylamide gel. [ LOADING VOLUMES] 3~5uL per well for mini gel; 7uL per well for large gel. Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels. [ QUALITY CONTROL] The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands. [IMPORTANT NOTES] 1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation. 2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%. 3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer. 4. Don't load too much protein. See recommended load volumes in the manual. 5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins. 6. If additional bands are observed in the gel image of the protein ladder, this might be caused by Dn oxidation in the storage buffer. 7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer. 8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding. [ SHELF LIFE] Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.)

Osteonectin (ON), Polyclonal Antibody (Cat# AAA2005086)

Full Name

Biotin-Linked Antibody to Osteonectin (ON)

Gene Names

SPARC; ON

Reactivity

Human

Applications

ICC, IHC, IHC-f, IHC-P, IF, WB

Purity

Affinity Chromatography

Pricing

$165/0.1 mL | $205/0.2 mL | $340/0.5 mL | $420/1 mL | $1,210/5x1 mL

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Structural Formula

SDS-PAGE ([ DESCRIPTION] Effective Size Range: 10kDa to 70kDa. Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa. Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use. [ STORAGE BUFFER] 62.5mM Tris-H3P04 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol. [APPLICATION] Accurate protein sizing on SDS-polyacrylamide gels and Western blots. [USAGE] 1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20°C have returned to solution. Do not boil! 2. Load the following volumes of the Marker on a SDS-polyacrylamide gel. [ LOADING VOLUMES] 3~5uL per well for mini gel; 7uL per well for large gel. Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels. [ QUALITY CONTROL] The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands. [IMPORTANT NOTES] 1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation. 2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%. 3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer. 4. Don't load too much protein. See recommended load volumes in the manual. 5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins. 6. If additional bands are observed in the gel image of the protein ladder, this might be caused by Dn oxidation in the storage buffer. 7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer. 8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding. [ SHELF LIFE] Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.)

Androstenedione (ASD), Polyclonal Antibody (Cat# AAA2014872)

Full Name

Biotin-Linked Antibody to Androstenedione (ASD)

Reactivity

General

Applications

IHC/ IHC-P/ IHC-F, EIA, WB, ICC

Purity

Affinity Chromatography.

Pricing

$190/0.1 mL | $240/0.2 mL | $425/0.5 mL | $515/1 mL | $1,430/5x1 mL

NDRG1, Blocking Peptide (Cat# AAA426596)

Full Name

NDRG1 Immunizing Peptide

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Mouse, Rat, Dog

Pricing

$215/0.1 mg

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SDS-PAGE

SDS-PAGE ([ DESCRIPTION] Effective Size Range: 10kDa to 70kDa. Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa. Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use. [ STORAGE BUFFER] 62.5mM Tris-H3P04 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol. [APPLICATION] Accurate protein sizing on SDS-polyacrylamide gels and Western blots. [USAGE] 1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20°C have returned to solution. Do not boil! 2. Load the following volumes of the Marker on a SDS-polyacrylamide gel. [ LOADING VOLUMES] 3~5uL per well for mini gel; 7uL per well for large gel. Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels. [ QUALITY CONTROL] The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands. [IMPORTANT NOTES] 1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation. 2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%. 3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer. 4. Don't load too much protein. See recommended load volumes in the manual. 5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins. 6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer. 7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer. 8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding. [ SHELF LIFE] Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.)

Deoxycholate (DC), Polyclonal Antibody (Cat# AAA2001803)

Full Name

Polyclonal Antibody to Deoxycholate (DC)

Reactivity

General

Applications

ICC, IHC, EIA, WB

Purity

Affinity Chromatography

Pricing

$180/0.1 mL | $220/0.2 mL | $390/0.5 mL | $475/1 mL | $1,320/5x1 mL

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Western Blot

SDS-PAGE ([ DESCRIPTION] Effective Size Range: 10kDa to 70kDa. Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa. Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use. [ STORAGE BUFFER] 62.5mM Tris-H3P04 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol. [APPLICATION] Accurate protein sizing on SDS-polyacrylamide gels and Western blots. [USAGE] 1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20°C have returned to solution. Do not boil! 2. Load the following volumes of the Marker on a SDS-polyacrylamide gel. [ LOADING VOLUMES] 3~5uL per well for mini gel; 7uL per well for large gel. Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels. [ QUALITY CONTROL] The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands. [IMPORTANT NOTES] 1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation. 2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%. 3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer. 4. Don't load too much protein. See recommended load volumes in the manual. 5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins. 6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer. 7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer. 8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding. [ SHELF LIFE] Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.)

Kallikrein 6 (KLK6), Polyclonal Antibody (Cat# AAA2004619)

Full Name

Biotin-Linked Antibody to Kallikrein 6 (KLK6)

Reactivity

Mouse

Applications

IHC, EIA, WB, ICC

Purity

Affinity Chromatography

Pricing

$165/0.1 mL | $210/0.2 mL | $350/0.5 mL | $430/1 mL | $1,235/5x1 mL

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Western Blot

SDS-PAGE ([ DESCRIPTION] Effective Size Range: 10kDa to 70kDa. Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa. Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use. [ STORAGE BUFFER] 62.5mM Tris-H3P04 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol. [APPLICATION] Accurate protein sizing on SDS-polyacrylamide gels and Western blots. [USAGE] 1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20°C have returned to solution. Do not boil! 2. Load the following volumes of the Marker on a SDS-polyacrylamide gel. [ LOADING VOLUMES] 3~5uL per well for mini gel; 7uL per well for large gel. Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels. [ QUALITY CONTROL] The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands. [IMPORTANT NOTES] 1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation. 2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%. 3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer. 4. Don't load too much protein. See recommended load volumes in the manual. 5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins. 6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer. 7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer. 8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding. [ SHELF LIFE] Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.)

Thrombospondin 1 (THBS1), Polyclonal Antibody (Cat# AAA2003532)

Full Name

Biotin-Linked Antibody to Thrombospondin 1 (THBS1)

Gene Names

THBS1; TSP; THBS; TSP1; TSP-1; THBS-1

Reactivity

Human

Applications

IHC, EIA, WB, ICC

Purity

Affinity Chromatography

Pricing

$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $420/1 mL | $1,215/5x1 mL

< >

<< Previous Next >>

Western Blote

SDS-PAGE ([ DESCRIPTION] Effective Size Range: 10kDa to 70kDa. Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa. Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use. [ STORAGE BUFFER] 62.5mM Tris-H3P04 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol. [APPLICATION] Accurate protein sizing on SDS-polyacrylamide gels and Western blots. [USAGE] 1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20°C have returned to solution. Do not boil! 2. Load the following volumes of the Marker on a SDS-polyacrylamide gel. [ LOADING VOLUMES] 3~5uL per well for mini gel; 7uL per well for large gel. Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels. [ QUALITY CONTROL] The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands. [IMPORTANT NOTES] 1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation. 2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%. 3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer. 4. Don't load too much protein. See recommended load volumes in the manual. 5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins. 6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer. 7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer. 8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding. [ SHELF LIFE] Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.)

Arginase (ARG), Monoclonal Antibody (Cat# AAA2007423)

Full Name

Biotin-Linked Antibody to Arginase (ARG)

Reactivity

Human

Applications

IHC, EIA, WB

Purity

Affinity Chromatography

Pricing

$175/0.1 mL | $220/0.2 mL | $380/0.5 mL | $465/1 mL | $1,300/5x1 mL

Ebola Virus, Monoclonal Antibody (Cat# AAA310371)

Full Name

MAb to Ebola Virus

Applications

EIA/ELISA, Western Blot

Purity

>=95% pure. Protein G chromatography

Pricing

$775/1 mg | $3,455/5x1 mg

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Western Blot (WB) (Western Blot analysis of extracts from C2C12 cells, untreated or treated with L7751 LY294002, insulin, or both using MBS6011344 (upper) or total NDRG1 antibody (lower).)

N-myc Downstream Regulated Gene 1 Protein, Monoclonal Antibody (Cat# AAA6011344)

Full Name

N-myc Downstream Regulated Gene 1 Protein, phosphorylated (Thr346) (NDRG1, Ndr1, Ndrl, Protein NDRG1, Protein Ndr1, CAP43, CMT4D, DRG1, GC4, HMSNL, NMSL, PROXY1, RIT42, RTP, TARG1, Tdd5)

Gene Names

NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

Reactivity

Human, Monkey, Mouse, Rat

Applications

WB, IP, IHC, ICC, FC/FACS, IF

Purity

Purified
Purified

Pricing

$705/0.1 mL | $3,170/5x0.1 mL

< >

<< Previous Next >>

Western Blot

SDS-PAGE ([ DESCRIPTION] Effective Size Range: 10kDa to 70kDa. Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa. Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use. [ STORAGE BUFFER] 62.5mM Tris-H3P04 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol. [APPLICATION] Accurate protein sizing on SDS-polyacrylamide gels and Western blots. [USAGE] 1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20°C have returned to solution. Do not boil! 2. Load the following volumes of the Marker on a SDS-polyacrylamide gel. [ LOADING VOLUMES] 3~5uL per well for mini gel; 7uL per well for large gel. Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels. [ QUALITY CONTROL] The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands. [IMPORTANT NOTES] 1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation. 2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%. 3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer. 4. Don't load too much protein. See recommended load volumes in the manual. 5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins. 6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer. 7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer. 8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding. [ SHELF LIFE] Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.)

Growth Differentiation Factor 11 (GDF11), Polyclonal Antibody (Cat# AAA2014017)

Full Name

Biotin-Linked Antibody to Growth Differentiation Factor 11 (GDF11)

Gene Names

GDF11; BMP11; BMP-11

Reactivity

Human

Applications

IHC, EIA, WB, ICC

Pricing

$175/0.1 mL | $220/0.2 mL | $375/0.5 mL | $460/1 mL | $1,295/5x1 mL

HCV Core Protein, Monoclonal Antibody (Cat# AAA592259)

Full Name

mAb anti-HCV Core Protein

Reactivity

Human hepatitis C virus. Others not tested
It showed no cross reaction with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).

Applications

EIA, WB

Purity

Protein G affinity purified

Pricing

$320/0.1 mg | $440/0.5 mg | $1,735/5x0.5 mg

< >

<< Previous Next >>

Structural Formula

SDS-PAGE ([ DESCRIPTION] Effective Size Range: 10kDa to 70kDa. Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa. Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use. [ STORAGE BUFFER] 62.5mM Tris-H3P04 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol. [APPLICATION] Accurate protein sizing on SDS-polyacrylamide gels and Western blots. [USAGE] 1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20°C have returned to solution. Do not boil! 2. Load the following volumes of the Marker on a SDS-polyacrylamide gel. [ LOADING VOLUMES] 3~5uL per well for mini gel; 7uL per well for large gel. Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels. [ QUALITY CONTROL] The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands. [IMPORTANT NOTES] 1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation. 2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%. 3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer. 4. Don't load too much protein. See recommended load volumes in the manual. 5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins. 6. If additional bands are observed in the gel image of the protein ladder, this might be caused by DTT oxidation in the storage buffer. 7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer. 8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding. [ SHELF LIFE] Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.)

Vitamin D3 (VD3), Polyclonal Antibody (Cat# AAA2001576)

Full Name

Biotin-Linked Antibody to Vitamin D3 (VD3)

Reactivity

General

Applications

IHC, EIA, WB, ICC

Purity

Affinity Chromatography

Pricing

$190/0.1 mL | $240/0.2 mL | $425/0.5 mL | $515/1 mL | $1,430/5x1 mL

Ep-CAM (CD326), Monoclonal Antibody (Cat# AAA570121)

Full Name

Mouse anti Ep-CAM (CD326)

Gene Names

EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; GA733-2; TACSTD1

Reactivity

Human

Applications

FC, IHC, IHC, frozen, IHC, paraffin, WB

Pricing

$460/0.1 mg | $2,025/5x0.1 mg

HCV Core Protein, Monoclonal Antibody (Cat# AAA592218)

Full Name

mAb anti-HCV Core Protein

Reactivity

Human hepatitis C virus. Others not tested
It showed no cross reaction with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).

Applications

EIA, WB, IF

Purity

Protein G affinity purified

Pricing

$320/0.1 mg | $440/0.5 mg | $1,735/5x0.5 mg

HCV NS-3, Monoclonal Antibody (Cat# AAA592001)

Full Name

mAb anti-HCV NS-3

Reactivity

Human hepatitis C virus. Others not tested
No cross reaction with HCV polyprotein core region and other non-structural regions.

Applications

EIA, WB

Purity

Protein G affinity purified

Pricing

$320/0.1 mg | $440/0.5 mg | $1,735/5x0.5 mg

Ebola Virus, Monoclonal Antibody (Cat# AAA310399)

Full Name

MAb to Ebola Virus

Applications

EIA/ELISA, Western Blot

Purity

>=95% pure (SDS-PAGE). Protein G chromatography

Pricing

$775/1 mg | $3,455/5x1 mg

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189 results for " Reducing Agents" - showing 1-50

Dehydroepiandrosterone (DHEA), Polyclonal Antibody (Cat# AAA2006064)

Cellular Glutathione Peroxidase, Assay Kit (Cat# AAA480417)

VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody (Cat# AAA438785)

VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody (Cat# AAA438814)

Protein, Assay Kit (Cat# AAA9711521)

CD81, Monoclonal Antibody (Cat# AAA212343)

NDRG1, cDNA Clone (Cat# AAA1274185)

CD81, Monoclonal Antibody (Cat# AAA212342)

NpFR1, Small Molecule (Cat# AAA807939)

N-Myc Downstream Regulated 1, Active Protein (Cat# AAA143793)

VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody (Cat# AAA4381331)

NDRG1-T346, Polyclonal Antibody (Cat# AAA9135645)

CD81, Monoclonal Antibody (Cat# AAA215271)

NDRG1, Polyclonal Antibody (Cat# AAA9607518)

NDRG1, Monoclonal Recombinant Antibody (Cat# AAA7114934)

NDRG1, Polyclonal Antibody (Cat# AAA127039)

NDRG1, Monoclonal Antibody (Cat# AAA2400638)

NDRG1, Polyclonal Antibody (Cat# AAA2519964)

NDRG1, Polyclonal Antibody (Cat# AAA9401419)

NDRG1, Polyclonal Antibody (Cat# AAA249784)

NDRG1-S330, Polyclonal Antibody (Cat# AAA9135660)

VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody (Cat# AAA4381330)

NDRG1, Polyclonal Antibody (Cat# AAA2529197)

NDRG1, Polyclonal Antibody (Cat# AAA8247520)

NDRG1, Active Protein (Cat# AAA203387)

NDRG1, Polyclonal Antibody (Cat# AAA9206039)

NDRG1, Polyclonal Antibody (Cat# AAA420361)

H1 (A/NewCaledonia/20/99)(H1N1), Monoclonal Antibody (Cat# AAA430026)

HA (B/Tokyo/53/99), Monoclonal Antibody (Cat# AAA430056)

lipl32, Recombinant Protein (Cat# AAA505042)

Phosphodiesterase 1, Enzyme (Cat# AAA653995)

Nucleoprotein, Monoclonal Antibody (Cat# AAA430051)

Rubella (E1), Monoclonal Antibody (Cat# AAA312834)

H3 (A/Shangdong/9/93) (H3N2), Monoclonal Antibody (Cat# AAA430034)

Osteonectin (ON), Polyclonal Antibody (Cat# AAA2005086)

Androstenedione (ASD), Polyclonal Antibody (Cat# AAA2014872)

NDRG1, Blocking Peptide (Cat# AAA426596)

Deoxycholate (DC), Polyclonal Antibody (Cat# AAA2001803)

Kallikrein 6 (KLK6), Polyclonal Antibody (Cat# AAA2004619)

Thrombospondin 1 (THBS1), Polyclonal Antibody (Cat# AAA2003532)

Arginase (ARG), Monoclonal Antibody (Cat# AAA2007423)

Ebola Virus, Monoclonal Antibody (Cat# AAA310371)

N-myc Downstream Regulated Gene 1 Protein, Monoclonal Antibody (Cat# AAA6011344)

Growth Differentiation Factor 11 (GDF11), Polyclonal Antibody (Cat# AAA2014017)

HCV Core Protein, Monoclonal Antibody (Cat# AAA592259)

Vitamin D3 (VD3), Polyclonal Antibody (Cat# AAA2001576)

Ep-CAM (CD326), Monoclonal Antibody (Cat# AAA570121)

HCV Core Protein, Monoclonal Antibody (Cat# AAA592218)

HCV NS-3, Monoclonal Antibody (Cat# AAA592001)

Ebola Virus, Monoclonal Antibody (Cat# AAA310399)

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