Immunofluorescence (IF)
(Fluorescence behavior of NpFR1 (SIH-181) in the oxidized (black) and reduced (red) forms, using 50 uM. Excitation: 405 or 488 nm (not shown). Emission: 490 - 600 nm, with peak at 545 nm.)
NpFR1 small molecule
NpFR1
Immunofluorescence (IF)
(Fluorescence behavior of NpFR1 (SIH-181) in the oxidized (black) and reduced (red) forms, using 50 uM. Excitation: 405 or 488 nm (not shown). Emission: 490 - 600 nm, with peak at 545 nm.)
Immunofluorescence (IF)
(Fluorescence behavior of NpFR1 (SIH-181) in the oxidized (black) and reduced (red) forms, using 50 uM. Excitation: 405 or 488 nm (not shown). Emission: 490 - 600 nm, with peak at 545 nm.)
Testing Data
(Chemical structure of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor. This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
Testing Data
(Chemical structure of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor. This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
Immunofluorescence (IF)
(Fluorescence emission of NpFR1 (SIH-181, 5 mM, lex = 405 nm) with the incremental addition of sodium dithionite. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
Immunofluorescence (IF)
(Fluorescence emission of NpFR1 (SIH-181, 5 mM, lex = 405 nm) with the incremental addition of sodium dithionite. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
Immunofluorescence (IF)
(Fluorescence response of NpFR1 (SIH-181) to cycles of oxidation and reduction. Reduction was achieved with sodium dithionite (100mM) followed by re-oxidation with 250mM H2O2. Spectra were recorded 5 min after the addition of reducing and oxidising agents. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
Immunofluorescence (IF)
(Fluorescence response of NpFR1 (SIH-181) to cycles of oxidation and reduction. Reduction was achieved with sodium dithionite (100mM) followed by re-oxidation with 250mM H2O2. Spectra were recorded 5 min after the addition of reducing and oxidising agents. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
Testing Data
(Imaging of NpFR1 (SIH-181) in 3T3-L1 preadipocytes treated with (a) vehicle control (b) H2O2 (100 mM, 2 min), (c) NpFR1 (50 mM, 2 h) and (d) NpFR1 (50 mM, 2 h) followed by H2O2 (100 mM, 2 min). Scale bar represents 50 mm, lex = 405 nm. (e) Integrated emission from 510 nm to 610 nm. Values are the mean ratio generated from the intensity from five fields of cells. Error bars represent standard error measurement (s.e.m.). The layout of this image was modified for optimal display from the original Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
Testing Data
(Imaging of NpFR1 (SIH-181) in 3T3-L1 preadipocytes treated with (a) vehicle control (b) H2O2 (100 mM, 2 min), (c) NpFR1 (50 mM, 2 h) and (d) NpFR1 (50 mM, 2 h) followed by H2O2 (100 mM, 2 min). Scale bar represents 50 mm, lex = 405 nm. (e) Integrated emission from 510 nm to 610 nm. Values are the mean ratio generated from the intensity from five fields of cells. Error bars represent standard error measurement (s.e.m.). The layout of this image was modified for optimal display from the original Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
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Product Notes
The NpFR1 (Catalog #AAA807939) is a Small Molecule produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NpFR1, Small Molecule" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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