Rabbit anti-Human, Mouse NDRG1 Polyclonal Antibody | anti-NDRG1 antibody

NDRG1 Antibody

Cat. Num. AAA9401419

• Gene Names: NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: NDRG1; Polyclonal Antibody; NDRG1 Antibody; CAP43; CMT4D; DRG1; GC4; HMSNL; anti-NDRG1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total NDRG1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 394

• Applicable Applications for anti-NDRG1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human NDRG1.
• Target Name: NDRG1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NDRG1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse lung using NDRG1 antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat brain using NDRG1 antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat kidney using NDRG1 antibody at dilution of 1:100 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using NDRG1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-NDRG1 antibody

N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3.

Product Categories/Family for anti-NDRG1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 207028748
• NCBI GeneID: 10397
• NCBI Accession #: NP_001128714.1
• NCBI GenBank Nucleotide #: NM_001135242.1
• UniProt Accession #: Q92597; O15207; Q6IBG2; Q9NYR6; Q9UK29; B3KR80; B7Z446
• Molecular Weight: 33,650 Da
• NCBI Official Full Name: protein NDRG1 isoform 1
• NCBI Official Synonym Full Names: N-myc downstream regulated 1
• NCBI Official Symbol: NDRG1
• NCBI Official Synonym Symbols: GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
• NCBI Protein Information: protein NDRG1
• UniProt Protein Name: Protein NDRG1
• Protein Family: Protein
• UniProt Gene Name: NDRG1
• UniProt Synonym Gene Names: CAP43; DRG1; RTP; DRG-1; RTP
• UniProt Entry Name: NDRG1_HUMAN

NCBI Description

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D).

Protein type: Cell development/differentiation; Vesicle

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: microtubule cytoskeleton; centrosome; microtubule; cell-cell adherens junction; perinuclear region of cytoplasm; recycling endosome membrane; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: gamma-tubulin binding; protein binding; cadherin binding; microtubule binding; Rab GTPase binding

Biological Process: response to metal ion; DNA damage response, signal transduction by p53 class mediator; myelin maintenance in the peripheral nervous system; mast cell activation

Disease: Charcot-marie-tooth Disease, Type 4d