Mouse anti-Human Ep-CAM (CD326) Monoclonal Antibody | anti-EPCAM antibody
Mouse anti Ep-CAM (CD326)
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; GA733-2; TACSTD1
Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
Ep-CAM (CD326); Monoclonal Antibody; Mouse anti Ep-CAM (CD326); anti-EPCAM antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
VU1D9
Specificity
VU1D9 reacts with the first EGF repeat in the extracellular domain of Ep-CAM. The epitope is sensitive for reducing agents. VU1D9 detects Ep-CAM in most simple, pseudostRatified and transitional epithelia and in the majority of Human carcinomas.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-EPCAM antibody
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
VU1D9 is suitable for immunoblotting (non-reducing conditions), immunocytochemistry and immuno-histochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
VU1D9 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with an NCI-H69 small cell lung carcinoma cell line.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-EPCAM antibody
Ep-CAM is a 40 kD glycoprotein and can be detected at the basolateral membrane of the majority of epithelial tissues, where it is intriCately linked with the cadherin-Catenin complex and hence the fundamental WNT pathway responsible for intracellular signalling and polarity. This antigen functions as a homotypic calcium-independent cell adhesion molecule. Of particular interest, Ep-CAM appears to be overexpressed by the majority of Human epithelial carcinomas, including colorectal, breast, prostate, head and neck, and hepatic carcinomas. The antigen is being used as a target for immunotherapy of Human carcinomas. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial prolifeRation.
Product Categories/Family for anti-EPCAM antibody
References
Litvinov, S.V., Velders, M.P., Bakker, H.A.M., Fleuren, G., Warnaar, S.O. (1994). Ep-CAM: a Human epithelial antigen Is a homophilic cell-cell adhesion molecule. J.Cell.Biol. 125, 437-46. Balzar M., Winter, M. J., de Boer, C. J., Litvinov S. V. (1999). The biology of the 17-1A antigen (Ep-CAM). J Mol Med 77, 699-712 Went, P.T.H, Lugli, A., Meier, S., Bundi, M., Mirlacher, M., Sauter, G., Dirnhofer, S. (2004). Frequent EpCam protein expression in Human carcinomas. Human Pathology 35, 122-28. Baeuerle, P.A. and Gires, O. (2007). EpCAM (CD326) finding its role in cancer. Br J Cancer 96, 417-23. Brunner, A., Prelog, M., Verdorfer, I., Tzankov, A., Mikuz, A., Ensinger, C. (2007). EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol 61, 307-10.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,932 Da
NCBI Official Full Name
Epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
EPCAM
NCBI Official Synonym Symbols
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; GA733-2; TACSTD1
NCBI Protein Information
epithelial cell adhesion molecule; OTTHUMP00000159073; OTTHUMP00000200307; OTTHUMP00000200426; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein)
UniProt Protein Name
Epithelial cell adhesion molecule
Protein Family
UniProt Gene Name
EPCAM
UniProt Synonym Gene Names
GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1
UniProt Entry Name
EPCAM_HUMAN
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Product Notes
The EPCAM epcam (Catalog #AAA570121) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Ep-CAM (CD326) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ep-CAM (CD326) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). VU1D9 is suitable for immunoblotting (non-reducing conditions), immunocytochemistry and immuno-histochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the EPCAM epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ep-CAM (CD326), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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