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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
90 results for " Perikaryon" - showing 1-50
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded R. brain section using CB2 Antibody(C-term). MBS9203911 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
CB2, Polyclonal Antibody (Cat# AAA9203911)
Full Name
CB2 Antibody (C-term)
Gene Names
CNR2; CB2; CX5; CB-2
Reactivity
Human
Applications
EIA, IHC, FC/FACS, WB
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with FMR1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
FMR1, Polyclonal Antibody (Cat# AAA9207834)
Full Name
FMR1 Antibody (N-term)
Gene Names
FMR1; POF; FMRP; POF1; FRAXA
Reactivity
Human, mouse
Applications
IHC-P, WB, FC,E
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(TMEM100 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TMEM100 antibody detected the TMEM100 protein (arrow).)
TMEM100, Polyclonal Antibody (Cat# AAA9212673)
Full Name
TMEM100 Antibody (C-term)
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of LRRK2 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
LRRK2, Polyclonal Antibody (Cat# AAA9604182)
Full Name
LRRK2 Antibody
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of APP (arrow) using rabbit polyclonal APP Antibody (N-term) (RB05012). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP gene (Lane 2) (Origene Technologies).)
APP, Polyclonal Antibody (Cat# AAA9212747)
Full Name
APP Antibody (N-term)
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Mouse (Predicted Reactivity: Monkey)
Applications
WB, EIA, IHC-p, FC
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of CNR2 using COLO205 whole cell lysates)
CNR2, Polyclonal Antibody (Cat# AAA9608963)
Full Name
CNR2 Antibody
Gene Names
CNR2; CB2; CX5; CB-2
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GNB2L1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
GNB2L1, Polyclonal Antibody (Cat# AAA9133435)
Full Name
GNB2L1 Polyclonal Antibody
Gene Names
RACK1; H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SIRT2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
SIRT2, Polyclonal Antibody (Cat# AAA9134119)
Full Name
SIRT2 Polyclonal Antibody
Gene Names
SIRT2; SIR2; SIR2L; SIR2L2
Reactivity
Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot-AZIN2 Polyclonal Antibody)
AZIN2, Polyclonal Antibody (Cat# AAA9140629)
Full Name
AZIN2 Polyclonal Antibody
Gene Names
AZIN2; ADC; AZI2; ODCp; AZIB1; ODC-p; ODC1L
Reactivity
Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CPNE6 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
CPNE6, Polyclonal Antibody (Cat# AAA9134390)
Full Name
CPNE6 Polyclonal Antibody
Reactivity
Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot-DRP2 Polyclonal Antibody)
DRP2, Polyclonal Antibody (Cat# AAA9140408)
Full Name
DRP2 Polyclonal Antibody
Gene Names
DRP2; DRP-2
Reactivity
Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CNR2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
CNR2, Polyclonal Antibody (Cat# AAA9135580)
Full Name
CNR2 Polyclonal Antibody
Gene Names
CNR2; CB2; CX5; CB-2
Reactivity
Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot-AZIN2 Polyclonal Antibody)
AZIN2, Polyclonal Antibody (Cat# AAA9140628)
Full Name
AZIN2 Polyclonal Antibody
Gene Names
AZIN2; ADC; AZI2; ODCp; AZIB1; ODC-p; ODC1L
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot detection of CDK5 (N-terminus) in 3T3, Hela, PC-12, COS7, Jurkat and K562 lysates using Cdk5 mouse mAb (1:500 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.Exposure time:5min.)
CDK5, Monoclonal Antibody (Cat# AAA475069)
Full Name
Anti-CDK5 (N-term) Mouse mAb
Gene Names
CDK5; LIS7; PSSALRE
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, ICC
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Testing Data
(Immunocytochemistry of HeLa cells using anti- CDK5(N-terminus) antibody diluted 1:150.)
CDK5, Monoclonal Antibody (Cat# AAA857768)
Full Name
CDK5 Antibody
Gene Names
CDK5; LIS7; PSSALRE
Reactivity
Human
Applications
WB, ICC
Purity
Affinity purified
Pricing
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
SDS-Page
Synaptopodin (SYNPO), Recombinant Protein (Cat# AAA2086821)
Full Name
Recombinant Synaptopodin (SYNPO)
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
> 97%
Pricing
$180/0.01 mg | $335/0.05 mg | $515/0.1 mg | $630/0.2 mg | $1,215/0.5 mg | $1,810/1 mg | $4,440/5 mg
Western Blot (WB)
(Western blot analysis of APP-pY756 in T47D cell line lysates (35ug/lane). APP (arrow) was detected using the purified Pab.)
APP-Y756, Polyclonal Antibody (Cat# AAA9209531)
Full Name
APP-Y756 Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Human (Predicted Reactivity: Monkey, Mouse, Pig, Rat)
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Anti-PARK8 (LRRK2) Antibody at 1:1000 dilution + Mouse brain lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 286 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
PARK8 (LRRK2), Monoclonal Antibody (Cat# AAA9200016)
Full Name
PARK8 (LRRK2) Antibody
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Reactivity
Human, mouse
Applications
WB, EIA
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
SDS-Page
Synaptopodin (SYNPO), Recombinant Protein (Cat# AAA2011993)
Full Name
Recombinant Synaptopodin (SYNPO)
Applications
WB, EIA, IP
Purity
> 95%
Pricing
$180/0.01 mg | $335/0.05 mg | $515/0.1 mg | $630/0.2 mg | $1,215/0.5 mg | $1,810/1 mg | $4,440/5 mg
Cannabinoid Receptor II, Positive Control (Cat# AAA542600)
Full Name
Cannabinoid Receptor II Antibody Western Blot Positive Control
Gene Names
Cnr2; CB2; CB-2; CB2-R
Applications
WB
Pricing
$335/5 Applications | $1,350/5x5 Applications
Cannabinoid Receptor II, Polyclonal Antibody (Cat# AAA541151)
Full Name
Cannabinoid Receptor II Antibody FITC-Conjugated
Gene Names
Cnr2; CB2; CB-2; CB2-R
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
NEURL, Blocking Peptide (Cat# AAA9227820)
Full Name
NEURL Antibody (Center) Blocking Peptide
Gene Names
NEURL1; neu; NEUR1; NEURL; RNF67; neu-1; bA416N2.1
Cannabinoid Receptor II, Polyclonal Antibody (Cat# AAA541150)
Full Name
Cannabinoid Receptor II Antibody BIOTIN-Conjugated
Gene Names
Cnr2; CB2; CB-2; CB2-R
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Cannabinoid Receptor 2, Polyclonal Antibody (Cat# AAA541149)
Full Name
Cannabinoid Receptor II Antibody Affinity Purified
Gene Names
Cnr2; CB2; CB-2; CB2-R
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB)
(Western blot detection of SirT2 in PC-12, Rat Brain and Mouse Brain cell lysates using SirT2 mouse mAb (1:1000 diluted). Predicted band size: 39,43KDa. Observed band size:39,43KDa.)
SirT2, Monoclonal Antibody (Cat# AAA9231168)
Full Name
Anti-SirT2 Antibody
Gene Names
SIRT2; SIR2; SIR2L; SIR2L2
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Pricing
$415/0.1 mL | $1,625/5x0.1 mL
Western Blot (WB)
(SLC8A3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SLC8A3 antibody detected the SLC8A3 protein (arrow).)
SLC8A3, Polyclonal Antibody (Cat# AAA9206025)
Full Name
SLC8A3 Antibody (C-term)
Gene Names
SLC8A3; NCX3
Reactivity
Human
Applications
WB, EIA, IHC-p
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
KCNA1, Blocking Peptide (Cat# AAA9221061)
Full Name
KCNA1 Antibody (Center) Blocking Peptide
Gene Names
KCNA1; EA1; MK1; AEMK; HBK1; HUK1; MBK1; RBK1; KV1.1
CTNND2, Blocking Peptide (Cat# AAA9219218)
Full Name
CTNND2 Antibody (C-term) Blocking peptide
Gene Names
CTNND2; GT24; NPRAP
TMEM100, Blocking Peptide (Cat# AAA9218582)
Full Name
TMEM100 Antibody (C-term) Blocking peptide
Western Blot (WB)
(Western blot analysis of Amyloid-βA4 (Phospho-Thr729) using Hela whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
APP, Polyclonal Antibody (Cat# AAA9614454)
Full Name
Phospho-APP (Thr729) Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Horse (100%), Chicken (100%), Xenopus (86%)
Predicted Reactivity: Pig (100%), Horse (100%), Chicken (100%), Xenopus (86%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
NDN, Blocking Peptide (Cat# AAA9221192)
Full Name
NDN Antibody (N-term) Blocking Peptide
Gene Names
NDN; PWCR; HsT16328
CB2, Blocking Peptide (Cat# AAA9217774)
Full Name
CB2 Antibody (C-term) Blocking peptide
Gene Names
CNR2; CB2; CX5; CB-2
NDN, Blocking Peptide (Cat# AAA9229516)
Full Name
NDN Blocking Peptide (N-term)
Gene Names
NDN; PWCR; HsT16328
Cannabinoid Receptor II, Blocking Peptide (Cat# AAA542555)
Full Name
Cannabinoid Receptor II Antibody Antigenic Blocking Peptide
Gene Names
Cnr2; CB2; CB-2; CB2-R
Applications
Immmunodepletion, Competition Assay
Pricing
$280/0.25 mg | $1,110/5x0.25 mg
CDK5, Blocking Peptide (Cat# AAA9229690)
Full Name
CDK5 Blocking Peptide (C-term)
Gene Names
CDK5; LIS7; PSSALRE
PARK8 (LRRK2) (E519), Blocking Peptide (Cat# AAA9226584)
Full Name
PARK8 (LRRK2) Antibody (E519) Blocking Peptide
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
CDK5, Blocking Peptide (Cat# AAA9229498)
Full Name
CDK5 Blocking Peptide (C-term)
Gene Names
CDK5; LIS7; PSSALRE
GNB2L1, Blocking Peptide (Cat# AAA9227503)
Full Name
GNB2L1 Antibody (N-term) Blocking Peptide
Gene Names
RACK1; H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
LRRK2, Blocking Peptide (Cat# AAA9229819)
Full Name
LRRK2 Blocking Peptide (C-term)
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
HDAC6, Blocking Peptide (Cat# AAA9218103)
Full Name
HDAC6 Antibody (C-term) Blocking Peptide
Gene Names
HDAC6; HD6; JM21; CPBHM; PPP1R90
PARK8 (LRRK2) (L893), Blocking Peptide (Cat# AAA9226585)
Full Name
PARK8 (LRRK2) Antibody (L893) Blocking Peptide
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Western Blot (WB)
(Western blot analysis of LRRK2 expression in U87-MG cell lysate (MBS179201).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRK2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LRRK2 )
LRRK2, Monoclonal Antibody (Cat# AAA179201)
Full Name
Anti-LRRK2 Rabbit Monoclonal Antibody
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
PARK8 (LRRK2) (L899), Blocking Peptide (Cat# AAA9226586)
Full Name
PARK8 (LRRK2) Antibody (L899) Blocking Peptide
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
PARK8 (LRRK2) (L955), Blocking Peptide (Cat# AAA9226587)
Full Name
PARK8 (LRRK2) Antibody (L955) Blocking Peptide
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
SYNPO, Blocking Peptide (Cat# AAA9220690)
Full Name
SYNPO Antibody (C-term) Blocking Peptide
CNR2, Blocking Peptide (Cat# AAA9229800)
Full Name
CNR2 Blocking Peptide (N-term)
Gene Names
CNR2; CB2; CX5; CB-2
KLHL24, Blocking Peptide (Cat# AAA9225577)
Full Name
KLHL24 Antibody (Center) Blocking peptide
Gene Names
KLHL24; DRE1; KRIP6
Western Blot (WB)
(Western blot analysis of CDK5 expression in HeLa cell lysate (MBS179347).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK5 )
CDK5, Monoclonal Antibody (Cat# AAA179347)
Full Name
Anti-CDK5 Rabbit Monoclonal Antibody
Gene Names
CDK5; LIS7; PSSALRE
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of SIRT2 expression in SKBR-3 cell lysate (MBS179323).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SIRT2 )
SIRT2/Sirtuin 2, Monoclonal Antibody (Cat# AAA179323)
Full Name
Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody
Gene Names
SIRT2; SIR2; SIR2L; SIR2L2
Reactivity
Human, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of SIRT2 expression in SH-SY5Y cell lysate (MBS179322).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SIRT2 )
SIRT2/Sirtuin 2, Monoclonal Antibody (Cat# AAA179322)
Full Name
Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody
Gene Names
SIRT2; SIR2; SIR2L; SIR2L2
Reactivity
Human, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
