Rabbit anti-Human, Mouse AZIN2 Polyclonal Antibody | anti-AZIN2 antibody

AZIN2 Polyclonal Antibody

Cat. Num. AAA9140628

• Gene Names: AZIN2; ADC; AZI2; ODCp; AZIB1; ODC-p; ODC1L
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: AZIN2; Polyclonal Antibody; AZIN2 Polyclonal Antibody; ADC; AZI2; AZIB1; ODC-p; ODC1L; ODCp; antizyme inhibitor 2; anti-AZIN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1.74 mg/ml (varies by lot)
• Sequence Length: 460

• Applicable Applications for anti-AZIN2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of human AZIN2 (NP_443724.1).
• Immunogen Sequence: MAGYLSESDFVMVEEGFSTRDLLKELTLGASQATTDEVAAFFVADLGAIVRKHFCFLKCLPRVRPFYAVKCNSSPGVLKVLAQLGLGFSCANKAEMELVQHIGIPASKIICANPCKQIAQIKYAAKHGIQLLSFDNEMELAKVVKSHPSAKMVLCIATDDSHSLSCLSLKFGVSLKSCRHLLENAKKHHVEVVGVSFHIGSGCPDPQAYAQSIADARLVFEMGTELGHKMHVLDLGGGFPGTEGAKVRFEEIASVINSALDLYFPEGCGVDIFAELGRYYVTSAFTVAVSIIAKKEVLLDQPGREEENGSTSKTIVYHLDEGVYGIFNSVLFDNICPTPILQKKPSTEQPLYSSSLWGPA
• Positive Samples: U-87MG, Mouse Pancreas, Mouse Kidney
• Cellular Location: Cell Projection, Cytoplasm, Cytoplasmic Granule, Cytoplasmic Vesicle, Endoplasmic Reticulum-Golgi Intermediate Compartment, Golgi Apparatus, Membrane, Nucleus, Perikaryon, Axon, Cis-Golgi Network, Dendrite, Perinuclear Region, Trans-Golgi Network
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot-AZIN2 Polyclonal Antibody)

Related Product Information for anti-AZIN2 antibody

The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 2, the second member of this gene family. Like antizyme inhibitor 1, antizyme inhibitor 2 interacts with all 3 antizymes and stimulates ODC activity and polyamine uptake. However, unlike antizyme inhibitor 1, which is ubiquitously expressed and localized in the nucleus and cytoplasm, antizyme inhibitor 2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Recent studies indicate that antizyme inhibitor 2 is also expressed in specific cell types in ovaries, adrenal glands and pancreas, and in mast cells. The exact function of this gene is not known, however, available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of antizyme inhibitor 2 has also been observed in brains of patients with Alzheimer's disease. There has been confusion in literature and databases over the nomenclature of this gene, stemming from an earlier report that a human cDNA clone (identical to ODCp/AZIN2) had arginine decarboxylase (ADC) activity (PMID:14738999). Subsequent studies in human and mouse showed that antizyme inhibitor 2 was devoid of arginine decarboxylase activity (PMID:19956990). Alternatively spliced transcript variants have been described for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 649572333
• NCBI GeneID: 113451
• NCBI Accession #: NP_001280491.1
• NCBI GenBank Nucleotide #: NP_001280491.1
• UniProt Accession #: Q96A70
• Molecular Weight: Calculated: 22kDa; 39kDa; 40kDa; 49kDa; 51kDa; Observed: 50kDa
• NCBI Official Full Name: antizyme inhibitor 2 isoform 1
• NCBI Official Synonym Full Names: antizyme inhibitor 2
• NCBI Official Symbol: AZIN2
• NCBI Official Synonym Symbols: ADC; AZI2; ODCp; AZIB1; ODC-p; ODC1L
• NCBI Protein Information: antizyme inhibitor 2
• UniProt Protein Name: Antizyme inhibitor 2
• Protein Family: Antizyme inhibitor
• UniProt Gene Name: AZIN2
• UniProt Synonym Gene Names: ADC; KIAA1945; ODCP; AzI2; ADC; ARGDC; ODC-like protein; ODC-p

NCBI Description

The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 2, the second member of this gene family. Like antizyme inhibitor 1, antizyme inhibitor 2 interacts with all 3 antizymes and stimulates ODC activity and polyamine uptake. However, unlike antizyme inhibitor 1, which is ubiquitously expressed and localized in the nucleus and cytoplasm, antizyme inhibitor 2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Recent studies indicate that antizyme inhibitor 2 is also expressed in specific cell types in ovaries, adrenal glands and pancreas, and in mast cells. The exact function of this gene is not known, however, available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of antizyme inhibitor 2 has also been observed in brains of patients with Alzheimer's disease. There has been confusion in literature and databases over the nomenclature of this gene, stemming from an earlier report that a human cDNA clone (identical to ODCp/AZIN2) had arginine decarboxylase (ADC) activity (PMID:14738999). Subsequent studies in human and mouse showed that antizyme inhibitor 2 was devoid of arginine decarboxylase activity (PMID:19956990). Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

ADC: Decarboxylates L-arginine to agmatine. Truncated splice isoforms probably lack activity. Belongs to the Orn/Lys/Arg decarboxylase class-II family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; EC 4.1.1.19; Lyase; Mitochondrial

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: axon; cis-Golgi network; cytoplasmic vesicle; cytosol; dendrite; ER-Golgi intermediate compartment membrane; nucleus; perikaryon; perinuclear region of cytoplasm; trans-Golgi network; transport vesicle

Molecular Function: arginine decarboxylase activity; ornithine decarboxylase activator activity; protein binding; putrescine transmembrane transporter activity

Biological Process: negative regulation of protein catabolic process; positive regulation of catalytic activity; putrescine biosynthetic process from ornithine; putrescine transport