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Application
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42 results for " Other nuclear bodies" - showing 1-42
Western Blot (WB)
(RASGRP2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow).)
RASGRP2, Polyclonal Antibody (Cat# AAA9207263)
Full Name
RASGRP2 Antibody (N-term)
Gene Names
RASGRP2; CDC25L; CALDAG-GEFI
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
WB, EIA, IHC-P, IF
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(Rabbit Anti-HERC5 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Pineal TissueObserved Staining: Cytoplasmic in cell bodies and processes of pinealocytesPrimary Antibody Concentration: 1:100Other Working Concentrations: 1/600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
HERC5, Polyclonal Antibody (Cat# AAA3206765)
Full Name
HERC5 antibody - middle region
Gene Names
HERC5; CEB1; CEBP1
Reactivity
Cow, Horse, Human, Pig, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis on COS7 cell lysate using PIAS2 Antibody, The lane on the left is treated with the antigen-specific peptide.
)
PIAS2, Polyclonal Antibody (Cat# AAA9600210)
Full Name
PIAS2 Antibody
Gene Names
PIAS2; DIP; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of RNF6 using MCF7 whole cell lysates)
RNF6, Polyclonal Antibody (Cat# AAA9609207)
Full Name
RNF6 Antibody
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9608077 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PIAS2, Polyclonal Antibody (Cat# AAA9608077)
Full Name
PIAS2 Antibody
Gene Names
PIAS2; DIP; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Rabbit Anti-PTOV1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Pineal TissueObserved Staining: Cytoplasmic and membrane in cell bodies and processes of pinealocytes and processes of intersticial cellsPrimary Antibody Concentration: 1:100Other Working Concentrations: 1/600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
PTOV1, Polyclonal Antibody (Cat# AAA3210009)
Full Name
PTOV1 antibody - N-terminal region
Gene Names
PTOV1; ACID2; PTOV-1
Reactivity
Cow, Dog, Human, Mouse, Pig, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Anti-PIASx1/2 Antibody (Q115) at 1:1000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
PIAS2, Polyclonal Antibody (Cat# AAA9217082)
Full Name
PIAS2 (PIASx1/2) Antibody (N-term)
Gene Names
PIAS2; DIP; MIZ; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4; PIASX-BETA; PIASX-ALPHA
Reactivity
Human; Predicted Reactivity: Mouse, Rat
Applications
WB, IHC, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(Rabbit Anti-MTUS1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Pineal TissueObserved Staining: Cytoplasmic and membrane in cell bodies of pinealocytes and their processesPrimary Antibody Concentration: 1:100Other Working Concentrations: 1/600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
MTUS1, Polyclonal Antibody (Cat# AAA3207359)
Full Name
MTUS1 antibody - middle region
Gene Names
MTUS1; ATBP; ATIP; ICIS; MP44; ATIP3; MTSG1
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Testing Data
(Blot of bovine retinal extracts probed with MBS6011560. The antibody stains a band corresponing to retinal arrestin at about 48kD.)
S-Arrestin, Monoclonal Antibody (Cat# AAA6011560)
Full Name
S-Arrestin (SAG, S-AG, 48kD Protein, Retinal S-antigen, Rod Photoreceptor Arrestin)
Gene Names
SAG; RP47; S-AG
Reactivity
Bovine, Human, Porcine, Rat
Applications
WB, IF
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$565/0.1 mL | $2,535/5x0.1 mL
PML, Monoclonal Antibody (Cat# AAA533769)
Full Name
PML antibody
Gene Names
PML; MYL; RNF71; PP8675; TRIM19
Applications
DB, ICC
Purity
PML antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Prostate Apoptosis Response Protein 4, Polyclonal Antibody (Cat# AAA616948)
Full Name
Prostate Apoptosis Response Protein 4 (Par4 Induced by Effectors of Apoptosis, PAWR, PRKC Apoptosis WT1 Regulator Protein, Prostate Apoptosis Response 4 Protein, Prostate Apoptosis Response Protein PAR-4, Transcriptional Repressor PAR4, WT1 Interacting Pr
Gene Names
PAWR; PAR4; Par-4
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$560/0.1 mL | $2,505/5x0.1 mL
Western Blot (WB)
(HSF1 Antibody western blot analysis in Jurkat cell line and mouse heart tissue lysates (35ug/lane).This demonstrates the HSF1 antibody detected the HSF1 protein (arrow).)
HSF1 Sumoylation Site, Polyclonal Antibody (Cat# AAA9211638)
Full Name
HSF1 Sumoylation Site Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
IF, EIA, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Separase, Monoclonal Antibody (Cat# AAA604886)
Full Name
Separase (Separin, Caspase-like Protein ESPL1, ESP1, Extra Spindle Poles-like 1, Extra Spindle Poles-like 1 Protein, ESPL1, FLJ46492, KIAA0165, SSE)
Gene Names
ESPL1; ESP1; SEPA; FLJ46492; KIAA0165
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$580/0.1 mL | $2,595/5x0.1 mL
RNF6, Blocking Peptide (Cat# AAA9224072)
Full Name
RNF6 Antibody (C-term) Blocking Peptide
Western Blot (WB)
(Figure 1. Western blot analysis of SATB1 using anti-SATB1 antibody (MBS1750618).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SATB1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SATB1 at approximately 100KD. The expected band size for SATB1 is at 86KD. )
SATB1, Polyclonal Antibody (Cat# AAA1750618)
Full Name
Anti-SATB1 Picoband antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
PIAS2 (PIASx1), Blocking Peptide (Cat# AAA9219173)
Full Name
PIAS2 (PIASx1) Antibody (C-term) Blocking peptide
Gene Names
PIAS2; DIP; MIZ; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4; PIASX-BETA; PIASX-ALPHA
PIAS2 (PIASx1/2), Blocking Peptide (Cat# AAA9219155)
Full Name
PIAS2 (PIASx1/2) Antibody (N-term) Blocking peptide
Gene Names
PIAS2; DIP; MIZ; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4; PIASX-BETA; PIASX-ALPHA
ELF4, Blocking Peptide (Cat# AAA9226653)
Full Name
ELF4 Antibody (Center) Blocking Peptide
Gene Names
ELF4; MEF; ELFR
PIAS2 (PIASx1/2), Blocking Peptide (Cat# AAA9219165)
Full Name
PIAS2 (PIASx1/2) Antibody (C-term) Blocking peptide
Gene Names
PIAS2; DIP; MIZ; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4; PIASX-BETA; PIASX-ALPHA
Western Blot (WB)
(Western blot analysis of the lysates from RAW264.7cells using KAPC A/B antibody.)
PKA, Polyclonal Antibody (Cat# AAA8512077)
Full Name
PKA CAT Antibody
Gene Names
PRKACA; PKACA; PPNAD4
Reactivity
Human,Mouse,Rat
Applications
WB, IHC, IF
Purity
Immunogen affinity purified
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
ELF4, Blocking Peptide (Cat# AAA9219384)
Full Name
ELF4 Antibody (N-term) Blocking peptide
Gene Names
ELF4; MEF; ELFR
TRIM22, Blocking Peptide (Cat# AAA9224361)
Full Name
TRIM22 Antibody(N-term) Blocking peptide
Gene Names
TRIM22; RNF94; STAF50; GPSTAF50
Western Blot (WB)
(Western blot analysis of Torsin A expression in 293T cell lysate (MBS179644).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOR1A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TOR1A )
Torsin A, Monoclonal Antibody (Cat# AAA179644)
Full Name
Anti-Torsin A Rabbit Monoclonal Antibody
Gene Names
TOR1A; DQ2; DYT1
Reactivity
Human
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PABPN1 expression in HeLa cell lysate (MBS179854).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PABPN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PABPN1 )
PABPN1, Monoclonal Antibody (Cat# AAA179854)
Full Name
Anti-PABPN1 Rabbit Monoclonal Antibody
Gene Names
PABPN1; OPMD; PAB2; PABII; PABP2; PABP-2
Reactivity
Human, Mouse
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of PTEN using anti-PTEN antibody (MBS1750302).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,Lane 2: mouse liver tissue lysate,Lane 3: human A549 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTEN at approximately 54KD. The expected band size for PTEN is at 47KD. )
PTEN, Polyclonal Antibody (Cat# AAA1750302)
Full Name
Anti-PTEN Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of DDX58 using anti-DDX58 antibody (MBS1750356).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysate,Lane 2: rat thymus tissue lysate,Lane 3: mouse spleen tissue lysate,Lane 4: mouse thymus tissue lysate,Lane 5: mouse NIH3T3 whole Cell lysate,Lane 6: human Jurkat whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX58 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DDX58 at approximately 116KD. The expected band size for DDX58 is at 106KD. )
DDX58/Rig I, Polyclonal Antibody (Cat# AAA1750356)
Full Name
Anti-DDX58/Rig I Picoband Antibody
Gene Names
DDX58; RIGI; RIG-I; RLR-1; SGMRT2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-SIRT1 (S47) expression in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP (MBS1750182).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SIRT1 )
SIRT1, Monoclonal Antibody (Cat# AAA1750182)
Full Name
Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody
Gene Names
SIRT1; SIR2; SIR2L1; SIR2alpha
Reactivity
Human
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-HSF1 (S326) expression in HeLa cell lysates treated with heat (MBS1750227).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSF1 )
HSF1, Monoclonal Antibody (Cat# AAA1750227)
Full Name
Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-SIRT1 (T530) expression in (1) 293T cell lysate; (2) 293T cell treated with Calyculin A (MBS1750181).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SIRT1 )
SIRT1, Monoclonal Antibody (Cat# AAA1750181)
Full Name
Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody
Gene Names
SIRT1; SIR2; SIR2L1; SIR2alpha
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human thymus, using SATB1 Antibody(MBS179626)SATB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SATB1 Antibody (MBS179626)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
SATB1, Monoclonal Antibody (Cat# AAA179626)
Full Name
Anti-SATB1 Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of COIL using anti-COIL antibody (M02784).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COIL antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COIL. )
Coilin, Monoclonal Antibody (Cat# AAA1752233)
Full Name
Anti-Coilin Monoclonal Antibody
Gene Names
COIL; CLN80; p80-coilin
Reactivity
Human
Applications
FC/FACS, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Phospho-P62/SQSTM1(Ser28) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
SQSTM1/p62, Polyclonal Antibody (Cat# AAA9614520)
Full Name
Phospho-SQSTM1/p62 (Ser28) Antibody
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (83%), Bovine (83%), Rabbit (83%)
Predicted Reactivity: Pig (83%), Bovine (83%), Rabbit (83%)
Applications
WB, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
beta Amyloid 1-42, Polyclonal Antibody (Cat# AAA9629019)
Full Name
beta Amyloid 1-42 Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Human
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Rabbit Anti-LPIN1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Pineal TissueObserved Staining: Cytoplasmic and membrane in cell bodies and processes of pinealocytesPrimary Antibody Concentration: 1:100Other Working Concentrations: 1/600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
LPIN1, Polyclonal Antibody (Cat# AAA3211538)
Full Name
LPIN1 antibody - N-terminal region
Gene Names
LPIN1; PAP1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Yeast, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC)
(Rabbit Anti-WBP11 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Pineal TissueObserved Staining: Cytoplasmic in cell bodies of pinealocytes and their processesPrimary Antibody Concentration: 1:100Other Working Concentrations: 1/600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
WBP11, Polyclonal Antibody (Cat# AAA3208130)
Full Name
WBP11 antibody - N-terminal region
Gene Names
WBP11; NPWBP; SIPP1; WBP-11; PPP1R165
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, IP, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC)
(At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Beclin 1, Polyclonal Antibody (Cat# AAA9612661)
Full Name
Phospho-Beclin 1 (Ser295) Antibody
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, Mouse, Rat
Applications
IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
WDFY3, Polyclonal Antibody (Cat# AAA9630048)
Full Name
WDFY3 Antibody
Gene Names
WDFY3; ALFY; ZFYVE25
Reactivity
Human, Mouse
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Chk2 (Phospho-Ser379) using EGF treated HuvEc whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Chk2, Polyclonal Antibody (Cat# AAA9614413)
Full Name
Phospho-Chk2 (Ser379) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%), Xenopus (100%)
Applications
WB, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
LSG1, Polyclonal Antibody (Cat# AAA9630467)
Full Name
LSG1 Antibody
Reactivity
Human, Mouse, Rat
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
YTHDC1, Polyclonal Antibody (Cat# AAA9629575)
Full Name
YTHDC1 Antibody
Gene Names
YTHDC1; YT521; YT521-B
Reactivity
Human, Rat
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
YTHDC1, Polyclonal Antibody (Cat# AAA9629042)
Full Name
YTHDC1 Antibody
Gene Names
YTHDC1; YT521; YT521-B
Reactivity
Human, Mouse, Rat
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Trim5 alpha, Polyclonal Antibody (Cat# AAA9630854)
Full Name
Trim5 alpha Antibody
Gene Names
TRIM5; RNF88; TRIM5alpha
Reactivity
Human
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
