Typical Testing Data/Standard Curve (for reference only)
Human dipeptidyl peptldase IV ELISA Kit | DPPIV elisa kit
Human dipeptidyl peptldase IV ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This DPP€ enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forDPP€. Standards or samples are then added to the microtiter plate wells andDPP€ if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofDPP€ present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forDPP€ are added to each well to "sandwich" theDPP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containDPP€ and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq]
Uniprot Description
DPP4: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Belongs to the peptidase S9B family. DPPIV subfamily.
Protein type: Cell surface; Cell adhesion; Membrane protein, integral; EC 3.4.14.5; Protease
Chromosomal Location of Human Ortholog: 2q24.3
Cellular Component: intercellular canaliculus; focal adhesion; cell surface; membrane; lamellipodium; lysosomal membrane; endocytic vesicle; apical plasma membrane; plasma membrane; integral to membrane; lipid raft
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protease binding; serine-type peptidase activity; dipeptidyl-peptidase activity; serine-type endopeptidase activity; receptor binding
Biological Process: regulation of cell-cell adhesion mediated by integrin; T cell activation; T cell costimulation; positive regulation of cell proliferation; response to hypoxia; endothelial cell migration; proteolysis
Research Articles on DPPIV
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Product Notes
The Human DPPIV dpp4 (Catalog #AAA732577) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA732577 ELISA Kit recognizes Human DPPIV. It is sometimes possible for the material contained within the vial of "dipeptidyl peptldase IV, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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