Rabbit anti-Human TPA Polyclonal Antibody | anti-TPA antibody

TPA (Tissue-type Plasminogen Activator, t-plasminogen Activator, t-PA, tPA, Alteplase, INN=Reteplase, Tissue-type Plasminogen Activator Chain A, Tissue-type Plasminogen Activator Chain B, PLAT) (AP)

Cat. Num. AAA6396951

• Gene Names: PLAT; TPA; T-PA
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TPA; Polyclonal Antibody; TPA (Tissue-type Plasminogen Activator; t-plasminogen Activator; t-PA; tPA; Alteplase; INN=Reteplase; Tissue-type Plasminogen Activator Chain A; Tissue-type Plasminogen Activator Chain B; PLAT) (AP); anti-TPA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLAT.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TPA antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PLAT, aa1-562 (NP_000921.1).
• Immunogen Sequence: MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARSYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PLAT expression in transfected 293T cell line by PLAT polyclonal antibody. Lane 1: PLAT transfected lysate (62.9kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-TPA antibody

PLAT (Plasminogen activator-also tPA/Tissue Plasminogen Activator) is a 64-69kD extracellular glycoprotein that belongs to the peptidase S1 family of enzymes. It is secreted by diverse cell types such as fibroblasts, endothelial cells and astrocytes. Unlike many other serine proteases, PLAT/tPA circulates as an active proenzyme. Following clot formation, both tPA and its substrate, plasminogen, bind to clot fibrin. Here, tPA converts plasminogen to plasmin, an enzyme that subsequently degrades the fibrin matrix of the clot. Plasmin also cleaves local tPA, generating a disulfide-linked, two chain tPA isoform whose activity is potentiated 5 to10 fold relative to the uncleaved form. tPa also cleaves PDGFCC and activates MMP9, suggesting an expanded role in vascular remodeling. Human PLAT/tPA is secreted as a 530aa single chain polypeptide (aa33-562). Depending upon its source, aa33-35 or aa33-38 may be removed in the circulation. PLAT contains one N-terminal fibronectin type I region (aa39-81), an EGD-like domain (aa82-120) a two kringle structures (127-208-215-296) and a peptidase S1 domain (aa311-561). Plasmin cleaves PLAT/tPA between aa310-311, generating an active disulfide-linked heterodimeric molecule that contains chains of 35kD and 33kD, respectively. There are multiple isoform variants. One contains a three aa substitution for aa140 coupled to a deletion of aa79-208, a second shows a Gly substitution for aa39-85, and a third possesses a 23aa substitution for aa269-562. Two additional isoforms shows a His substitution for aa54-134, and a 25aa substitution for aa269-562, respectively. Over aa36-562, human PLAT shares 81% aa sequence identity with mouse PLAT.

Product Categories/Family for anti-TPA antibody

Antibodies; Coagulation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4505861
• NCBI GeneID: 5327
• NCBI Accession #: NP_000921.1
• NCBI GenBank Nucleotide #: NM_000930.2
• UniProt Accession #: P00750; Q15103; Q503B0; Q6PJA5; Q7Z7N2; Q86YK8; Q9BU99; Q9BZW1; A8K022; B2R8E8
• Molecular Weight: 160,000
• NCBI Official Full Name: tissue-type plasminogen activator isoform 1 preproprotein
• NCBI Official Synonym Full Names: plasminogen activator, tissue
• NCBI Official Symbol: PLAT
• NCBI Official Synonym Symbols: TPA; T-PA
• NCBI Protein Information: tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA)
• UniProt Protein Name: Tissue-type plasminogen activator
• Protein Family: Taurine--pyruvate aminotransferase
• UniProt Gene Name: PLAT
• UniProt Synonym Gene Names: t-PA; t-plasminogen activator; tPA
• UniProt Entry Name: TPA_HUMAN

NCBI Description

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]