Rabbit anti-Human, Mouse CERK Polyclonal Antibody | anti-CERK antibody

CERK, CT (CERK, KIAA1646, Ceramide kinase, Acylsphingosine kinase, Lipid kinase 4)

Cat. Num. AAA641552

• Gene Names: CERK; LK4; hCERK; dA59H18.2; dA59H18.3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CERK; Polyclonal Antibody; CT (CERK; KIAA1646; Ceramide kinase; Acylsphingosine kinase; Lipid kinase 4); Anti -CERK; anti-CERK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CERK antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: CERK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495~524 amino acids from the C-terminal region of human CERK.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-CERK Pab is used in Western blot to detect CERK in mouse heart tissue lysate (Lane 1) and A2058 cell lysate (Lane 2).)

Related Product Information for anti-CERK antibody

Ceramide kinases convert the sphingolipid metabolite ceramide into ceramide-1-phosphate, both key mediators of cellular apoptosis and survival. Ceramide metabolism plays an essential role in the viability of neuronal cells, the membranes of which are particularly rich in sphingolipids. CERK catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. This enzyme acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8 dihydroceramide), and to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. High level expression is noted in heart, brain, skeletal muscle, kidney and liver; moderate expression in peripheral blood leukocytes and thymus; and low expression in spleen, small intestine, placenta and lung.

Product Categories/Family for anti-CERK antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 20336726
• NCBI GeneID: 64781
• NCBI Accession #: NP_073603.2
• NCBI GenBank Nucleotide #: NM_022766.5
• UniProt Accession #: Q8TCT0; Q9BYB3; Q9UGE5; A0JNT4; A8K611
• Molecular Weight: 59,977 Da
• NCBI Official Full Name: ceramide kinase
• NCBI Official Synonym Full Names: ceramide kinase
• NCBI Official Symbol: CERK
• NCBI Official Synonym Symbols: LK4; hCERK; dA59H18.2; dA59H18.3
• NCBI Protein Information: ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase
• UniProt Protein Name: Ceramide kinase
• Protein Family: Ceramide kinase
• UniProt Gene Name: CERK
• UniProt Synonym Gene Names: KIAA1646; hCERK; LK4
• UniProt Entry Name: CERK1_HUMAN

NCBI Description

CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]

Uniprot Description

CERK: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.

Protein type: EC 2.7.1.138; Kinase, lipid; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: mitochondrion; plasma membrane; integral to membrane

Molecular Function: ceramide kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: sphingolipid metabolic process; protein kinase C activation; glycosphingolipid metabolic process; ceramide metabolic process; lipid phosphorylation; transmembrane transport