Testing Data
PIP5K1B recombinant protein
PIP5K1B, Active
Gene Names
PIP5K1B; MSS4; STM7
Applications
Kinase Assay
Synonyms
PIP5K1B; Active; PIP5K1B recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
Full length
Sequence Length
2957
Applicable Applications for PIP5K1B recombinant protein
Kinase Assay
Species
Human
Tag
GST tag
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for PIP5K1B recombinant protein
Description: Recombinant full-length human PIP5K1B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PIP5K1B (phosphatidylinositol-4-phosphate 5-kinase, type I, beta) is a member of the phosphatidylinositol-4-phosphate 5-kinase family. PIP5K1B gene contains 17 exons and spans more than 300 kb. The seventeenth exon was found by RT-PCR, and are derived from the 3-prime untranslated region of the PRKACG gene which is located on 9q13 approximately 3 kb downstream of the STM7.I 3-prime untranslated region (1). The overexpression of PIP5K1B in COS-7 cells induces an increase in short actin fibers and a decrease in actin stress fibers (2).
Scientific Background: PIP5K1B (phosphatidylinositol-4-phosphate 5-kinase, type I, beta) is a member of the phosphatidylinositol-4-phosphate 5-kinase family. PIP5K1B gene contains 17 exons and spans more than 300 kb. The seventeenth exon was found by RT-PCR, and are derived from the 3-prime untranslated region of the PRKACG gene which is located on 9q13 approximately 3 kb downstream of the STM7.I 3-prime untranslated region (1). The overexpression of PIP5K1B in COS-7 cells induces an increase in short actin fibers and a decrease in actin stress fibers (2).
Product Categories/Family for PIP5K1B recombinant protein
References
1. Pook, M. A.et.al: Exon-intron structure of a 2.7-kb transcript of the STM7 gene with phosphatidylinositol-4-phosphate 5-kinase activity. Genomics 42: 170-172, 1997. 2. Ishihara, H.et.al: Type I phosphatidylinositol-4-phosphate 5-kinases: cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family. J. Biol. Chem. 273: 8741-8748, 1998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
96 kDa
NCBI Official Full Name
Homo sapiens phosphatidylinositol-4-phosphate 5-kinase type 1 beta (PIP5K1B), transcript variant 2, mRNA
NCBI Official Synonym Full Names
phosphatidylinositol-4-phosphate 5-kinase type 1 beta
NCBI Official Symbol
PIP5K1B
NCBI Official Synonym Symbols
MSS4; STM7
NCBI Protein Information
phosphatidylinositol 4-phosphate 5-kinase type-1 beta
UniProt Protein Name
Phosphatidylinositol 4-phosphate 5-kinase type-1 beta
Protein Family
UniProt Gene Name
PIP5K1B
UniProt Synonym Gene Names
STM7; PIP5K1-beta; PtdIns(4)P-5-kinase 1 beta; PIP5KIbeta
Uniprot Description
PIP5K1B: Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2. Together with PIP5K1A is required after stimulation of G-protein coupled receptors for stable platelet adhesion. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.68; Kinase, lipid
Chromosomal Location of Human Ortholog: 9q21.11
Cellular Component: cytosol; uropod
Molecular Function: 1-phosphatidylinositol-3-phosphate 5-kinase activity; 1-phosphatidylinositol-4-phosphate 5-kinase activity; protein binding
Biological Process: phosphatidylinositol biosynthetic process; regulation of phosphoinositide 3-kinase cascade
Research Articles on PIP5K1B
Similar Products
Product Notes
The PIP5K1B pip5k1b (Catalog #AAA515571) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full length. AAA Biotech's PIP5K1B can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the PIP5K1B pip5k1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIP5K1B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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