PIP5K1C active protein

PIP5K1C, Active

Cat. Num. AAA515572

• Gene Names: PIP5K1C; LCCS3; PIP5Kgamma; PIP5K-GAMMA; PIP5K1-gamma
• Purity: >80%
• Synonyms: PIP5K1C; Active; KIAA0589; LCCS3; PlP5K-GAMMA; PlP5Kgamma; PlPKlg_v4; PIP5K1C active protein

• Host: Sf9 cells
• Purity/Purification: >80%
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM, DTT, 0.1mM PMSF, and 25% glycerol.
• Concentration: 0.1 mg/ml (varies by lot)

• Host Note: Recombinant full length human protein expressed in Sf9 cells.
• Preparation and Storage: Ships on dry ice. Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Specific Activity

(The specific activity of PIP5K1C was determined to be 6,927 nmol/min/mg as per activity assay protocol.)

Purity

(The purity of PIP5K1C was detrermined to be >70% by densitometry approx. MW 120 kDa.)

Related Product Information for PIP5K1C active protein

Description: Recombinant full-length human PIP5K1C was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: PIP5K1C or phosphatidylinositol-4-phosphate 5-kinase 1 gamma phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1C is found at synapses and has been found to play roles in endocytosis and cell migration. PIP5K1C directly associates with beta-catenin and increases beta-catenin activity downstream of growth factor stimulation (1). PIP5K1C expression and kinase activity enhance beta-catenin phosphorylation on residues that promote nuclear importation and transcriptional activity. PIP5K1C deficiency impairs neutrophil recruitment as a result of defect in adhesion. PIP5K1C regulated the adhesion through facilitating RhoA GTPase and integrin activation by chemoattractants (2).

Product Categories/Family for PIP5K1C active protein

Enzymes; Active Lipid Kinases

References

1. Schramp M. et al: PIPKIgamma regulates beta-catenin transcriptional activity downstream of growth factor receptor signaling. Cancer Res. 2011 Feb 15;71 (4):1282-91. 2. Xu W, Integrin-induced PIP5K1C kinase polarization regulates neutrophil polarization, directionality, and in vivo infiltration. Immunity. 2010 Sep 24;33 (3):340-50.

NCBI and Uniprot Product Information

• NCBI GI #: 307691177
• NCBI GeneID: 23396
• NCBI Accession #: NM_012398.2
• NCBI GenBank Nucleotide #: NM_012398
• UniProt Accession #: O60331; Q7LE07; B7Z9E7; C6GIJ7; C6GIJ8
• NCBI Official Full Name: Homo sapiens phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (PIP5K1C), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 5-kinase type 1 gamma
• NCBI Official Symbol: PIP5K1C
• NCBI Official Synonym Symbols: LCCS3; PIP5Kgamma; PIP5K-GAMMA; PIP5K1-gamma
• NCBI Protein Information: phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
• Protein Family: Phosphatidylinositol 4-phosphate 5-kinase
• UniProt Gene Name: PIP5K1C
• UniProt Synonym Gene Names: KIAA0589; PIP5K1-gamma; PtdIns(4)P-5-kinase 1 gamma; PIP5KIgamma

NCBI Description

This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Sep 2010]

Uniprot Description

PIP5K1C: a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.68; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol; focal adhesion; nucleoplasm; uropod

Molecular Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; cell-cell adhesion; neutrophil chemotaxis; phagocytosis; phosphatidylinositol biosynthetic process; regulation of phosphoinositide 3-kinase cascade; synaptic vesicle endocytosis; synaptic vesicle exocytosis

Disease: Lethal Congenital Contracture Syndrome 3